全文获取类型
收费全文 | 219篇 |
免费 | 16篇 |
出版年
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 15篇 |
2014年 | 19篇 |
2013年 | 22篇 |
2012年 | 27篇 |
2011年 | 25篇 |
2010年 | 10篇 |
2009年 | 6篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有235条查询结果,搜索用时 15 毫秒
1.
Mammary explants from pregnant ewes were cultured in the presence of insulin, cortisol and prolactin, either alone, or in combination. After 2 d of culture, total RNA was extracted from explants and the content of beta-lactoglobulin mRNA was estimated using a specific labelled cDNA probe. The mRNA for beta-lactoglobulin was only deinduced slowly in the absence of hormone during the culture. Prolactin alone induced the accumulation of the mRNA. Insulin and cortisol added together were also stimulatory, but they only moderately amplified the prolactin effect. Beta-lactoglobulin gene in ewes, is therefore, controlled by the lactogenic hormones which also induce casein gene expression. The amplitude of the stimulation was unexpectedly low. This seems due in part to the fact that the gene was only deinduced weakly. In this respect, beta-lactoglobulin gene appears to be less dependent on lactogenic hormones under these experimental conditions than casein genes. 相似文献
2.
Hugues Puissant Martine Azoulay Jean-Louis Serre LucLarget Piet Claudine Junien 《Human genetics》1988,79(3):280-282
Summary Most patients with the complex association aniridia — predisposition to Wilms' tumor (WAGR syndrome) present with a de novo constitutional deletion of band 11p13. We report a patient with WAGR syndrome and a reciprocal translocation between chromosomes 5 and 11 t(5;11)(q11;p13). High resolution banding cytogenetic analysis and molecular characterization using 11p13 DNA markers showed a tiny deletion encompassing the gene for CAT but sparing the gene for FSHB. This suggests that syndromes associated with apparently balanced translocations may be due to undetectable loss of material at the breakpoint(s) rather than to breakage in the gene itself. 相似文献
3.
Tracy Ferea Emeline T. Contreras Thim Oung Emma J. Bowman Barry J. Bowman 《Molecular & general genetics : MGG》1994,242(1):105-110
We have isolated the cDNA and corresponding genomic DNA encoding citrate synthase in Neurospora crassa. Analysis of the protein coding region of this gene, named cit-1, indicates that it specifies the mitochondrial form of citrate synthase. The predicted protein has 469 amino acids and a molecular mass of 52002 Da. The gene is interrupted by four introns. Hybridization experiments show that a cit-1 probe binds to two different fragments of genomic DNA, which are located on different chromosomes. Neurospora crassa may have two isoforms of citrate synthase, one in the mitochondria and the other in microbodies. 相似文献
4.
Tracy Ferea Emeline T. Contreras Thim Oung Emma J. Bowman Barry J. Bowman 《Molecular genetics and genomics : MGG》1994,242(1):105-110
We have isolated the cDNA and corresponding genomic DNA encoding citrate synthase in Neurospora crassa. Analysis of the protein coding region of this gene, named cit-1, indicates that it specifies the mitochondrial form of citrate synthase. The predicted protein has 469 amino acids and a molecular mass of 52002 Da. The gene is interrupted by four introns. Hybridization experiments show that a cit-1 probe binds to two different fragments of genomic DNA, which are located on different chromosomes. Neurospora crassa may have two isoforms of citrate synthase, one in the mitochondria and the other in microbodies. 相似文献
5.
In vitro fertilization, development, and implantation after exposure of mature mouse oocytes to visible light. 总被引:1,自引:0,他引:1
P Barlow F Puissant P Van der Zwalmen J Vandromme P Trigaux F Leroy 《Molecular reproduction and development》1992,33(3):297-302
Mature mouse oocytes were exposed prior to in vitro fertilization to visible light during 1, 2, or 4 hr at an intensity of 4,000 lux. Compared to controls cultured under identical conditions but protected from light, exposed eggs did not show any significant modification of cleavage speed and rate. After transfer of blastocysts obtained in vitro in uteri of pseudopregnant females, the implantation rate and the proportion of normal fetuses were not found to be different in relation to preliminary light exposure of oocytes fertilized and cultured in vitro. 相似文献
6.
7.
Célia Parinot Quentin Rieu Jonathan Chatagnon Silvia C. Finnemann Emeline F. Nandrot 《Journal of visualized experiments : JoVE》2014,(94)
Analysis of one of the vital functions of retinal pigment epithelial (RPE) cells, the phagocytosis of spent aged distal fragments of photoreceptor outer segments (POS) can be performed in vitro. Photoreceptor outer segments with stacks of membranous discs containing the phototransduction machinery are continuously renewed in the retina. Spent POS are eliminated daily by RPE cells. Rodent, porcine/bovine and human RPE cells recognize POS from various species in a similar manner. To facilitate performing large series of experiments with little variability, a large stock of POS can be isolated from porcine eyes and stored frozen in aliquots. This protocol takes advantage of the characteristic of photopigments that display an orange color when kept in the dark. Under dim red light, retinae are collected in a buffer from opened eyecups cut in halves. The retinal cell suspension is homogenized, filtered and loaded onto a continuous sucrose gradient. After centrifugation, POS are located in a discrete band in the upper part of the gradient that has a characteristic orange color. POS are then collected, spun, resuspended sequentially in wash buffers, counted and aliquoted. POS obtained this way can be used for phagocytosis assays and analysis of protein activation, localization or interaction at various times after POS challenge. Alternatively, POS can be labeled with fluorophores, e.g., FITC, before aliquoting for subsequent fluorescence quantification of POS binding or engulfment. Other possible applications include the use of modified POS or POS challenge combined with stress conditions to study the effect of oxidative stress or aging on RPE cells. 相似文献
8.
Marie-Annette Carbonneau Emeline Cartron Claude-Louis Leger Christiane Senglat Bernard Descomps 《Free radical research》2013,47(2):127-142
Oxidized low density lipoprotein (LDL) plays an important role in atherogenesis. It is generally thought that LDL is mainly oxidized in the intima of vessel walls, surrounded by hydrophilic antioxidants and proteins such as albumin. The aim of this study was to investigate the possible interrelationships between oxidation resistance of LDL and its protein and lipid moieties. Proteins and to a lesser extent lipids, appeared to be the major determinants in the LDL Cu 2+ -oxidation resistance, which in turn depend on the ultracentrifugation (UC) procedure used. Comparing high speed/short time (HS/ST, 4 u h), high speed/long time (HS/LT, 6-16 u h) and low speed/long time (LS/LT, 24 u h) conditions of UC, HS with the shortest time (4 u h) led to prepare LDL (named LDL·HS-4 u h) with higher total protein and triglyceride contents, unchanged total cholesterol, phospholipids and Vitamin E, and higher Cu 2+ -oxidation resistance. Among proteins, only albumin allows to explain changes. PAF acetyl hydrolase appeared to be unaffected, whereas its pro-oxidant role was established and found only in the absence of albumin. In contrast the pro-oxidant role of caeruloplasmin took place regardless of the albumin content of LDL. The antioxidant effect of albumin (the oxidation lag time was doubled for 20 u mol/mol albumin per LDL) is assumed to be due to its capacity at decreasing LDL affinity for Cu 2+ . Interestingly, the LDL·HS-4 u h albumin content mirrored the intrinsic characteristics of LDL in the plasma and was not affected by added free albumin. Moreover, it has been verified that in 121 healthy subjects albumin was the best resistance predictor of the Cu 2+ -oxidation of LDL·HS-4 u h, with a multiple regression equation: lag time (min)=62.1+0.67(HSA/apoB)+0.02 (TG/apoB) m 0.01(TC/apoB); r =0.54, P <0.0001. Accounted for by lag time, the oxidation resistance did not correlate with f -tocopherol and ubiquinol contents of LDL. The mean albumin content was about 10 u mol/mol, and highly variable (0-58 u mol/mol) with subjects. The LDL·HS-4 u h may account for the status of LDL in its natural environment more adequately than LDL resulting from other conditions of UC. 相似文献
9.
Erwan Quéméré Fabrice Hibert Christian Miquel Emeline Lhuillier Emmanuel Rasolondraibe Julie Champeau Clément Rabarivola Louis Nusbaumer Cyrille Chatelain Laurent Gautier Patrick Ranirison Brigitte Crouau-Roy Pierre Taberlet Lounès Chikhi 《PloS one》2013,8(3)
In tropical regions, most primary ecosystems have been replaced by mosaic landscapes in which species must cope with a large shift in the distribution of their habitat and associated food resources. Primates are particularly vulnerable to habitat modifications. Most species persist in small fragments surrounded by complex human-mediated matrices whose structure and connectivity may strongly influence their dispersal and feeding behavior. Behavioral plasticity appears to be a crucial parameter governing the ability of organisms to exploit the resources offered by new matrix habitats and thus to persist in fragmented habitats. In this study, we were interested in the dietary plasticity of the golden-crowned sifaka (Propithecus tattersalli), an endangered species of lemur, found only in the Daraina region in north-eastern Madagascar. We used a DNA-based approach combining the barcoding concept and Illumina next-generation sequencing to (i) describe the species diet across its entire range and (ii) evaluate the influence of landscape heterogeneity on diet diversity and composition. Faeces from 96 individuals were sampled across the entire species range and their contents were analyzed using the trnL metabarcoding approach. In parallel, we built a large DNA reference database based on a checklist of the plant species of the Daraina region. Our results suggest that golden-crowned sifakas exhibit remarkable dietary diversity with at least 130 plant species belonging to 80 genera and 49 different families. We highlighted an influence of both habitat type and openness on diet composition suggesting a high flexibility of foraging strategies. Moreover, we observed the presence of numerous cultivated and naturalized plants in the faeces of groups living in forest edge areas. Overall, our findings support our initial expectation that P. tattersalli is able to cope with the current level of alteration of the landscape and confirm our previous results on the distribution and the dispersal ability of this species. 相似文献
10.
Sabrina H. van Ierssel Emeline M. Van Craenenbroeck Vicky Y. Hoymans Christiaan J. Vrints Viviane M. Conraads Philippe G. Jorens 《PloS one》2013,8(8)