全文获取类型
收费全文 | 1583篇 |
免费 | 205篇 |
出版年
2021年 | 28篇 |
2018年 | 8篇 |
2016年 | 20篇 |
2015年 | 33篇 |
2014年 | 38篇 |
2013年 | 53篇 |
2012年 | 97篇 |
2011年 | 63篇 |
2010年 | 49篇 |
2009年 | 39篇 |
2008年 | 67篇 |
2007年 | 59篇 |
2006年 | 73篇 |
2005年 | 73篇 |
2004年 | 66篇 |
2003年 | 71篇 |
2002年 | 60篇 |
2001年 | 57篇 |
2000年 | 60篇 |
1999年 | 51篇 |
1998年 | 17篇 |
1997年 | 15篇 |
1996年 | 18篇 |
1995年 | 10篇 |
1994年 | 12篇 |
1993年 | 22篇 |
1992年 | 27篇 |
1991年 | 35篇 |
1990年 | 32篇 |
1989年 | 31篇 |
1988年 | 25篇 |
1987年 | 24篇 |
1986年 | 35篇 |
1985年 | 31篇 |
1984年 | 26篇 |
1983年 | 19篇 |
1982年 | 34篇 |
1981年 | 13篇 |
1980年 | 20篇 |
1979年 | 15篇 |
1978年 | 18篇 |
1977年 | 14篇 |
1975年 | 13篇 |
1974年 | 15篇 |
1973年 | 11篇 |
1972年 | 18篇 |
1971年 | 13篇 |
1970年 | 10篇 |
1969年 | 15篇 |
1967年 | 9篇 |
排序方式: 共有1788条查询结果,搜索用时 0 毫秒
1.
2.
Stairway climbing provides a ubiquitous and inconspicuous method of burning calories. While typically two strategies are employed for climbing stairs, climbing one stair step per stride or two steps per stride, research to date has not clarified if there are any differences in energy expenditure between them. Fourteen participants took part in two stair climbing trials whereby measures of heart rate were used to estimate energy expenditure during stairway ascent at speeds chosen by the participants. The relationship between rate of oxygen consumption () and heart rate was calibrated for each participant using an inclined treadmill. The trials involved climbing up and down a 14.05 m high stairway, either ascending one step per stride or ascending two stair steps per stride. Single-step climbing used 8.5±0.1 kcal min−1, whereas double step climbing used 9.2±0.1 kcal min−1. These estimations are similar to equivalent measures in all previous studies, which have all directly measured The present study findings indicate that (1) treadmill-calibrated heart rate recordings can be used as a valid alternative to respirometry to ascertain rate of energy expenditure during stair climbing; (2) two step climbing invokes a higher rate of energy expenditure; however, one step climbing is energetically more expensive in total over the entirety of a stairway. Therefore to expend the maximum number of calories when climbing a set of stairs the single-step strategy is better. 相似文献
3.
Salmonella typhimurium ATCC 7136 exhibited major changes in lipid composition when grown in the presence of either 0.15% sodium deoxycholate or 0.15% sodium benzoate. These lipophilic compounds had directly opposing effects on the lipid profile of the organism. The saturated/unsaturated ratio was markedly elevated in benzoate-grown cells. On the other hand, it was depressed by an even greater margin from the control after growth in the presence of deoxycholate. Adjustments in the phospholipid content of the cells were also recorded. Phosphatidylethanolamines decreased by 28 and 50% in the deoxycholate- and benzoate-grown cells, respectively. Compensatory increases in phosphatidylglycerols of 87.5 and 175% occurred, along with increases in cardiolipins of 12- and 22-fold, respectively. Deoxycholate or benzoate supplementation also altered the relative distribution of neutral lipids; again, benzoate stimulated the greater change. Compositional changes were accompanied in the organism by increased heat sensitivity, but the effect on the susceptibility of S. typhimurium to injury varied with the physical properties of the supplement used. 相似文献
4.
5.
P J Watkins 《BMJ (Clinical research ed.)》1982,285(6339):425-427
6.
7.
Amira Alkharusi Elena Lesma Silvia Ancona Eloisa Chiaramonte Thomas Nystr?m Alfredo Gorio Gunnar Norstedt 《PloS one》2016,11(1)
Pulmonary lymphangioleiomyomatosis (LAM) is a rare lung disease caused by mutations in the tumor suppressor genes encoding Tuberous Sclerosis Complex (TSC) 1 and TSC2. The protein product of the TSC2 gene is a well-known suppressor of the mTOR pathway. Emerging evidence suggests that the pituitary hormone prolactin (Prl) has both endocrine and paracrine modes of action. Here, we have investigated components of the Prl system in models for LAM. In a TSC2 (+/-) mouse sarcoma cell line, down-regulation of TSC2 using siRNA resulted in increased levels of the Prl receptor. In human LAM cells, the Prl receptor is detectable by immunohistochemistry, and the expression of Prl in these cells stimulates STAT3 and Erk phosphorylation, as well as proliferation. A high affinity Prl receptor antagonist consisting of Prl with four amino acid substitutions reduced phosphorylation of STAT3 and Erk. Antagonist treatment further reduced the proliferative and invasive properties of LAM cells. In histological sections from LAM patients, Prl receptor immuno reactivity was observed. We conclude that the Prl receptor is expressed in LAM, and that loss of TSC2 increases Prl receptor levels. It is proposed that Prl exerts growth-stimulatory effects on LAM cells, and that antagonizing the Prl receptor can block such effects. 相似文献
8.
9.
10.
The nature of the human blood group P1 determinant 总被引:4,自引:0,他引:4