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1.
2.
The characterization of extracellular matrix molecules and their putative receptors is rapidly evolving in Drosophila. Where corresponding vertebrate and Drosophila extracellular proteins have been identified they are very similar with respect to their structural properties, suggesting a high degree of conservation during evolution. By contrast, indications for components homologous to vertebrate cell-cell adhesion molecules are still very sparse. Studies on the regulation of the Drosophila genes encoding cell adhesion molecules that are involved in general basic functions during morphogenesis, together with a knowledge of the function of the genes responsible for pattern formation, should lead towards a more complete understanding of the organism's developmental program. 相似文献
3.
Complex alternative splicing of acetylcholinesterase transcripts in Torpedo electric organ; primary structure of the precursor of the glycolipid-anchored dimeric form 总被引:16,自引:2,他引:14
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J L Sikorav N Duval A Anselmet S Bon E Krejci C Legay M Osterlund B Reimund J Massouli 《The EMBO journal》1988,7(10):2983-2993
4.
The role of dendritic cells in the initiation of immune responses to contact sensitizers. I. In vivo exposure to antigen 总被引:2,自引:0,他引:2
S C Knight J Krejci M Malkovsky V Colizzi A Gautam G L Asherson 《Cellular immunology》1985,94(2):427-434
Twenty-four hours after skin painting mice with picryl chloride (PIC) there was a four- to fivefold increase in the numbers of dendritic cells (DC) isolated from the lymph nodes. These DC initiated primary proliferative and cytotoxic responses when added to cultures of normal syngeneic lymph node cells. The proliferative response was enhanced when the donors of the responding lymph node cells were sensitized with the same antigen. Contact sensitivity developed in syngeneic mice injected into the footpads with 30,000-50,000 DC from lymph nodes of mice painted with picryl chloride 1 day previously. Thus, 1 day after skin painting mice, there were dendritic cells in the draining lymph nodes which were able both to initiate primary stimulation of lymphocytes in vitro and to sensitize recipient mice to give specific delayed hypersensitivity reactions. 相似文献
5.
Niels C. Krejci Lynne Smith Rebecca Rudd Robert Langdon Joseph McGuire 《In vitro cellular & developmental biology. Animal》1991,27(12):933-938
Summary To investigate the regulation of epithelial differentiation, normal human epidermal keratinocytes were cultured floating on
the surface of culture medium without attachment to a solid substrate. Keratinocytes spread out on the surface of the medium,
proliferated and differentiated either into several flat lacy sheets 1 to 3 cells thick (on medium containing 0.15 mM calcium) or formed one single aggregate of cells from 5 to 15 cells in thickness on medium containing 1.15 mM calcium. The cell aggregates demonstrated a pattern of ordered epithelial differentiation. Levels of progressive differentiation
resembling the structure of normal human epidermis were identified by light microscopy, immunohistochemistry, and electron
microscopy. Differentiation proceeded from cells at the air side toward cells at the medium side with basal appearing cells
on the air side and keratinocytes expressing filaggrin and involucrin on the side toward the medium. These results demonstrate
that organized epithelial differentiation can occur in the absence of extracellular matrix. In contrast with other air-liquid
interface cultures, epithelial differentiation in the absence of extracellular matrix progresses from air towards medium. 相似文献
6.
Mihola Ondrej Kobets Tatyana Krivankova Klara Linhartova Eliska Gasic Srdjan Schimenti John C. Trachtulec Zdenek 《Chromosoma》2020,129(1):69-82
Chromosoma - Long transgenes are often used in mammalian genetics, e.g., to rescue mutations in large genes. In the course of experiments addressing the genetic basis of hybrid sterility caused by... 相似文献
7.
Osami Akimitsu Kai Wada Teruki Noji Nozomi Taniwaki Milada Krejci Miyo Nakade Hitomi Takeuchi Tetsuo Harada 《Journal of physiological anthropology》2013,32(1):13
Background
This study aims to examine the relationship between tyrosine and phenylalanine intake at breakfast as precursors of dopamine, and scores on the Torsvall-Åkerstedt Diurnal Type Scale and of mental health in Japanese infants aged 2 to 5 years.Results
An integrated questionnaire was administered to parents of 1,367 infants attending one of ten nursery schools governed by Kochi City or a kindergarten affiliated with the Faculty of Education at Kochi University (775 answers for analysis: 56.7%) in May and June 2008. Questionnaires included the Torsvall-Åkerstedt Diurnal Type Scale and questions on sleep habits (onset, offset, quality, quantity, and so on), meal habits (content and regularity of timing), and mental health (depressive states). Amount of tyrosine and phenylalanine intake was calculated based on a breakfast content questionnaire and data on the components of amino acids in foods. Infants who ingested more than 800 mg of tyrosine or phenylalanine at breakfast per meal were more morning-type than those who ingested less than 800 mg (ANOVA: P= 0.005). However, this relationship disappeared in the ANCOVA analysis (with the covariance of tryptophan intake, P= 0.894). Infants who ingested more than 800 mg of the two amino acids at breakfast showed significantly higher mental health scores (lower frequency of depressive states) than those who ingested less than 800 mg (ANOVA: P = 0.004). This relationship remained significant when ANCOVA analysis was performed with the covariance of tryptophan (ANCOVA: P= 0.017).Conclusions
These results suggest that tyrosine and phenylalanine ingested at breakfast are not related with circadian phase, but are relate with mental health in infants. 相似文献8.
Petr Mlejnek Petr Kosztyu Petr Dolezel Yasuhisa Kimura Katerina Cizkova Eliska Ruzickova 《Journal of cellular biochemistry》2019,120(10):18406-18414
The interaction between ABCB1 transporter and its substrates takes place in cell membranes but the available data precludes quantitative analysis of the interaction between transporter and substrate molecules. Further, the amount of transporter is usually expressed as a number of ABCB1 molecules per cell. In contrast, the substrate concentration in cell membranes is estimated by determination of substrate-lipid partition coefficient, as examples. In this study, we demonstrate an approach, which enables us to estimate the concentration of ABCB1 molecules within plasma membranes. For this purpose, human leukemia K562 cells with varying expression levels of ABCB1 were used: drug selected K562/Dox and K562/HHT cells with very high transporter expression, and K562/DoxDR2, K562/DoxDR1, and K562/DoxDR05 cells with gradually decreased expression of ABCB1 derived from K562/Dox cells using RNA interference technology. First, we determined the absolute amount of ABCB1 in cell lysates using immunoblotting and recombinant ABCB1 as a standard. We then determined the relative portion of transporter residing in the plasma membrane using immunohistochemistry in nonpermeabilized and permeabilized cells. These results enabled us to estimate the concentration of ABCB1 in the plasma membrane in resistant cells. The ABCB1 concentrations in the plasma membrane of drug selected K562/Dox and K562/HHT cells containing the highest amount of transporter reached millimolar levels. Concentrations of ABCB1 in the plasma membrane of resistant K562/DoxDR2, K562/DoxDR1, and K562/DoxDR05 cells with lower transporter expression were proportionally decreased. 相似文献
9.
We have developed a simple method for creating defined deletions in yeast vectors by utilizing the ability of Saccharomyces cerevisiae to perform homologous recombination. Two complementary single-stranded oligonucleotides are designed so that the 5' and 3' halves of the resulting double-stranded oligonucleotide are homologous to the 5' and 3' side of a desired deletion junction, respectively. The sequence to be deleted is cleaved by restriction endonuclease digestion, followed by co-transformation of the linearized plasmid and the oligonucleotide into yeast. By homologous recombination in vivo, a subset of the plasmids will recircularize and simultaneously acquire the deletion as defined by the oligonucleotide. 相似文献
10.
Van Komen S Reddy MS Krejci L Klein H Sung P 《The Journal of biological chemistry》2003,278(45):44331-44337
Saccharomyces cerevisiae SRS2 encodes an ATP-dependent DNA helicase that is needed for DNA damage checkpoint responses and that modulates the efficiency of homologous recombination. Interestingly, strains simultaneously mutated for SRS2 and a variety of DNA repair genes show low viability that can be overcome by inactivating homologous recombination, thus implicating inappropriate recombination as the cause of growth impairment in these mutants. Here, we report on our biochemical characterization of the ATPase and DNA helicase activities of Srs2. ATP hydrolysis by Srs2 occurs efficiently only in the presence of DNA, with ssDNA being considerably more effective than dsDNA in this regard. Using homopolymeric substrates, the minimal DNA length for activating ATP hydrolysis is found to be 5 nucleotides, but a length of 10 nucleotides is needed for maximal activation. In its helicase action, Srs2 prefers substrates with a 3' ss overhang, and approximately 10 bases of 3' overhanging DNA is needed for efficient targeting of Srs2 to the substrate. Even though a 3' overhang serves to target Srs2, under optimized conditions blunt-end DNA substrates are also dissociated by this protein. The ability of Srs2 to unwind helicase substrates with a long duplex region is enhanced by the inclusion of the single-strand DNA-binding factor replication protein A. 相似文献