Features of energy metabolism of myocardial mitochondria and their ultrastructure in normal and immobilized rats of different animal social rank

The oxidative and phosphorylating functions of mitochondria (M) and their ultrastructure were studied in the myocardium of normal and 6.5-hour immobilized rats that belonged to different zoosocial groups. M from dominant rats under normal conditions were shown to exhibit higher energy and to possess better respiratory energy regulation than those of "outcast" rats. However, the ultrastructure of M had no group specificity in normal. The immobilization caused more profound changes in M from the dominant rats and led to a more pronounced swelling of M in the myocardium of the above rats than in the "outcast". M from the subdominant rats were most resistant to an immobilization stress.     

Identification of proteins specifically interacting with YB-1 mRNA 3′ UTR and the effect of hnRNP Q on YB-1 mRNA translation

In this study, proteins specifically interacting with the 3′ untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3′ UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator — YB-1 itself.     

Characteristics of the rat behavior following single and multiple exposures of the hypoglycemic insulin doses]

Rats behaviour in the "open field" changed in 2 hrs after a single episode of hypoglycaemia was abolished with glucose whereas no changes occurred in their stereotyped behaviour and intraspecies interaction. In 24 hrs quantitative parameters of the "open field" behaviour normalised although the behaviour still had an altered structure. At the same time, amphetamine-induced stereotyped behaviour's indices became reduced. These and other findings suggest some deeper behavioural changes in rats during their recovery from repeated hypoglycaemic episodes and absence of synchronisation of the insulin effects.     

Effect of cold on the antigen binding function of splenic cells at different periods of immunogenesis in rats

The patterns of changes in the immune response when deep cooling with different rates acted at various periods during development of the immune response, were examined in Wistar rats. Cold exposure causes not only a suppression but also stimulation of the immune response to antigen. The suppressive effect of deep cooling when it preceded immune challenge (the antigen injected at a body temperature decreased by 3-4 degrees C) waned and became stimulating with increasing time interval between antigen challenge and cold exposure. The stimulating effect on the immune response was most pronounced when cold exposure occurred in 5 days after the antigen challenge. These changes differed quantitatively also when cooling was either rapid, or slow. Thus the modulating effect of the thermal afferent signal was differently manifested depending in the cooling rate, i.e. the presence or absence of the dynamic activity of the peripheral thermosensitive afferents, and the time elapsed between antigen challenge and cold exposure.     

Serotonin binding by various populations of peritoneal cells and blood leukocytes from intact and thymectomized mice

Specific activity of 3H-serotonin binding with adhesive cells, as well as with T-lymphocyte enriched cells and B-lymphocytes, obtained from peritoneal excudate and blood leucocytes of CBA mice was determined. The highest affinity to amine was established in peritoneal T2 cells and adhesive blood cells. Results of studying the action of imipramin and blocators of some known serotonin receptors on amine binding with the investigated cells allow a conclusion on structural differences of serotonin-binding centres located on T-lymphocyte enriched cells and B-lymphocytes depending of their localization. The reduction of serotonin binding by immunocytes during ageing processes with animals (particularly tymectomized) was revealed, and this is supposed to be correlated with changes of immunological reactivity with ageing. It is supposed that serotonin participates in cooperative interactions of immunocytes in blood and peritoneal cavity.     

pH track of expanding bacterial populations

A method of pH distribution measurements in agar nutrient media containing expanding bacterial populations is described. It is based on measuring pH microsamples taken at different points of the media. The sample volume was 10 microliters. A pH sensitive field effect transistor was used as a measuring electrode. Acidification was found to occur in glucose media, while alkalization occurred in the media containing peptone.     

Antioxidant and electrochemical properties of dihydroquercetin monosuccinate,the novel water-soluble bioflavonoid derivative

Dihydroquercetin monosuccinate is a synthetic derivative of native antioxidant dihydroquercetin, which has a wide spectrum of pharmacological properties. Being more soluble in water than its precursor, the novel synthetic derivative of the bioflavonoid maintains a high antioxidant activity, which is confirmed by the TEAC method and the voltammetry data. The redox transformations of dihydriquercetin monosuccinate on a graphite electrode are more complicated as compared to the parent flavonoid.     

Lactic Acid Bacteria and Yeasts as Means of Decreasing Wine Acidity

Two hundred monocultures of lactic acid bacteria and 30 associations of yeasts and lactic acid bacteria have been studied. A stable association was developed which was capable of decreasing wine acidity. The association contained two species of bacteria, Leuconostoc oenosand Pediococcus pentosaceus, and the yeast Saccharomyces cerevisiae. The physiology of the microorganisms was studied, and their effects on the chemical composition of wines were determined.     

Polymorphism for supernumerary chromosomes in populations of Rana temporaria L

Occurrence of B-chromosomes in four populations of Rana temporaria L. in Minsk, its suburb and Berezinski? biosphere reserve has been studied. The percentage of individuals with B-chromosomes and the average number of B-chromosomes per genome are shown to multiply increase in the population inhabiting for 40 years in the region polluted with waste products. The results obtained are discussed from the standpoint of the action of mechanism for the accumulation of additional chromosomes due to environmental pollution.