全文获取类型
收费全文 | 650篇 |
免费 | 29篇 |
国内免费 | 2篇 |
出版年
2021年 | 5篇 |
2019年 | 8篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 14篇 |
2015年 | 17篇 |
2014年 | 20篇 |
2013年 | 41篇 |
2012年 | 37篇 |
2011年 | 38篇 |
2010年 | 22篇 |
2009年 | 23篇 |
2008年 | 30篇 |
2007年 | 26篇 |
2006年 | 22篇 |
2005年 | 12篇 |
2004年 | 22篇 |
2003年 | 29篇 |
2002年 | 20篇 |
2000年 | 5篇 |
1999年 | 8篇 |
1998年 | 7篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1988年 | 6篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 10篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1979年 | 15篇 |
1978年 | 10篇 |
1977年 | 11篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1974年 | 16篇 |
1973年 | 11篇 |
1972年 | 5篇 |
1971年 | 9篇 |
1970年 | 8篇 |
1969年 | 7篇 |
1968年 | 6篇 |
1966年 | 6篇 |
排序方式: 共有681条查询结果,搜索用时 15 毫秒
1.
2.
Margot S. Kruskall MD Elizabeth E. Eynon Zuheir Awdeh Chester A. Alper Edmond J. Yunis 《Immunogenetics》1987,26(4-5):216-219
The HLA class I antigen B44 is found in each of two different extended major histocompatibility haplotypes (allele combinations of HLA-B, HLA-DR, and complement genes BF, C2, C4A, and C4B in linkage disequilibrium). Using isoelectric focusing, two variants of HLA-B44 were identified. The basic variant was found in all cell lines with the extended haplotype HLA-B44, DR7, FC31, and the acidic variant in all cell lines with the extended haplotype HLA-B44, DR4, SC30. The occurrence of each antigen variant with a unique extended haplotype explains previous observations concerning the nonrandom association of B44 variants with DR antigens. 相似文献
3.
Dynamics of breathing in the hypoxic awake lamb 总被引:1,自引:0,他引:1
Cote A.; Yunis K.; Blanchard P. W.; Mortola J. P.; Bureau M. A. 《Journal of applied physiology》1988,64(1):354-359
Newborn mammals respond to hypoxia with an immediate hyperventilation that is rapidly dampened. Changes in mechanical properties of the respiratory system during hypoxia have been considered an important reason for this fall in minute ventilation (VE). We have studied the dynamic mechanical behavior of the respiratory system in eight unanesthetized intact newborn lambs (mean age 2 days) during normoxia and hypoxia (FIO2 = 0.08). Mouth pressure (P), airflow (V), and volume (V) were recorded while lambs were breathing through a leak-proof face mask and a pneumotachograph. Active compliance (C') and resistance (R') of the respiratory system were computed from P developed during an inspiratory effort against airway closure at end expiration and V and V of the preceding breaths. Tidal expiratory V-V curves were analyzed to estimate the elevation in functional residual capacity (FRC) over resting volume (Vr). After hypoxia, there was an immediate increase in VE in the first 2 min, from 0.49 to 1.13 l.kg-1.min-1, followed by a rapid decrease to 0.80. After 8 min of hypoxia, C' was unchanged. The inspiratory R' decreased during hypoxia, probably reflecting a drop in inspiratory laryngeal resistance. The expiratory V-V curves during hypoxia showed considerable braking, often with a double peak in expiratory V. This pattern was only occasionally seen during normoxia. In animals with a linear segment of the expiratory V-V curves the FRC-Vr difference could be calculated and averaged 1.93 ml/kg during normoxia and 3.47 during hypoxia. The recoil P of the respiratory system at end expiration was 0.75 cmH2O during normoxia vs. 1.63 cmH2O during hypoxia (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
4.
Gloria E. Egea Ivan Yunis Thomas Spies Jack Strominger Zuheir L. Awdeh Chester A. Alper Edmond J. Yunis 《Immunogenetics》1991,33(1):4-11
Genomic probes from the HLA-B region of the major histocompatibility complex (MHC) were used to study the association of restriction fragment length polymorphisms (RFLPs) with various MHC alleles, complotypes, and extended haplotypes. The two DNA probes, M20A and R5A, were derived from previously cloned cosmids and are located 38 and 110 kilobases (kb) centromeric to HLa-B, respectively. Five different RFLP variants occuring in five different haplotypic combinations were detected within a panel of 40 homozygous-typing cells and cells from 21 families using Bst EII. In two informative families with HLA-B/DR recombinations the inheritance of the RFLP variants was consistent with their mapping between HLA-B and complotypes. The R5A/M20A haplotypic pattern 6.5 kb/3.0 kb (A) had a normal Caucasian frequency of approximately 0.43 and was found in all independent examples of the extended haplotypes [HLA-B8,SC01,DR3], [HLA-B18,F1C30, DR3], [HLa-Bw62,SC33,Dr4], [HLa-B44,SC30,Dr4], and [HLA-Bw47,FC91,0DR7]. The patterns of 6.9 kb/ 3.0 kb (B), 6.5 kb/4.7 kb (C), 1.45 kb/3.0 kb (D), and 6.9 kb/4.7 kb (E) had normal Caucasian frequencies of approximately 0.23, 0.15, 0.15, and 0.04 and were found on all independent examples of [HLA-B38,SC21, DR4], [HLA-Bw57,SC61,DR7], [HLA-B7,SC31,DR2], and [HLA-B44,FC31,DR7], respectively. Individual complotypes or HLA-B alleles which were markers of extended haplotypes showed variable associations. For example, HLA-B7 and the complotype SC31 were associated with all R5A/M20A RFLP haplotypes except haplotype E, although [HLA-B7,SC31,DR7] was associated exclusively with haplotype D. HLA-B27, not known to be part of an extended haplotype, was suprisingly exclusively associated with the 6.5 kb/4.7 kb or C haplotypic pattern in all five instances tested. These findings support the concept of regional conservation of DNA on independent examples of extended haplotypes. The results also further characterize these haplotypes. 相似文献
5.
6.
Kristin M. G. Heirwegh Nirmalya Banerjee Karel van Nerum Edmond de Langhe 《Plant cell reports》1985,4(2):108-111
Somatic embryogenesis of Cichorium intybus L. var. Carolus is induced using cubical pieces of mature tap roots with an intervening callus phase. A Murashige and Skoog's (MS) semi solid basal medium supplemented with 2,4-dichlorophenoxyacetic acid (0.02 or 0.2 mg/l) and benzylaminopurine (0.25 mg/l) and a liquid MS medium devoid of growth regulators are used respectively for induction of callus and somatic embryoids and for further development and germination. Regeneration from the nodular proembryonal stage to the full grown embryoids occurs following different morphological pathways depending on the physical and chemical environment of the culture. Further development of these embryos into plantlets and the possibilities of application of this technique in plantbreeding have been discussed.Abbreviations MS
Murashige and Skoog medium
- BAP
benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid 相似文献
7.
Edmond A. Goidl Christiane Samarut Ansbert Schneider-Gadicke Neal L. Hochwald G.Jeanette Thorbecke Gregory W. Siskind 《Cellular immunology》1984,85(1):25-33
Using hapten-reversible inhibition of plaque formation as an assay for auto-anti-idiotype antibody (anti-Id) and as a means for following idiotype (Id) expression, we have obtained evidence that following immunization with trinitrophenyl (TNP) conjugates (a) there are differences in Id expression in the anti-TNP antibody response to different TNP conjugates although there is some overlap; (b) different strains, although showing some differences in Id expression, tend to produce cross-reactive Ids, thus no obvious allotype linked inheritance of Id expression is observed in this heterogeneous immune response; (c) the auto-anti-Id produced following immunization with TNP-Brucella abortus or TNP-Ficoll tends to be of the IgG2a and IgG2b isotypes. 相似文献
8.
9.
Immunopathology of aging 总被引:3,自引:0,他引:3
10.