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Abstract. Maintenance of realistically low solution P concentrations under controlled conditions is a major difficulty in studies of P nutrition. In this report, we describe a relatively simple and economical sand culture system capable of sustaining plant growth to maturity under controlled yet realistic P regimes. The system uses Al2 O3 as a solid-phase P buffer, and modern process control technology to control irrigation and addition of other mineral nutrients. Aspects of the design, use and potential applications of automated solid-phase systems are discussed. The system was used to grow Phaseolus vulgaris to matarity at 0.4 mmol m3 , 1.0 mmol m3 and 27 mmol m3 P with and without mycorrhizal inoculation. At flowering, low solution P concentrations were associated with reduced leaf concentrations of P in nonmycorrhizal plants, and reduced leaf concentrations of Ca in both mycorrhizal and nonmycorrhizal plants. Mycorrhizal inoculation increased leaf P, K, Mg and Mn concentrations, but reduced leaf N concentration. Low P regimes reduced final seed yield by diminishing both the number of pods per plant and the number of seeds per pod. Mycorrhizal inoculation significantly enhanced seed yield under low P regimes by increasing seed weight, the number of pods per plant, and the number of seeds per pod. 相似文献
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SLAVA S. EPSTEIN 《The Journal of eukaryotic microbiology》1994,41(5):500-503
ABSTRACT A new species of marine interstitial ciliate, Tracheloraphis primitarum sp. n., is described from intertidal sands (160-200-μm medium grain size) along the Northwest Atlantic coast. Living specimens are not transparent under incident light, 0.4-0.9 mm in length, and are characterized by an elongated body, bulbous anterior and rounded posterior regions. Cytostome is simple without a cleft on the lip. Protargol silver impregnation shows 18-29 longitudinal kineties. The glabrous zone is very narrow, 6-7 μm wide, and corresponds to the area occupied by one kinety and two interkinetal spaces. Ten to fifteen kineties terminate against the glabrous zone. Interkinetal extrusomes are present. The nuclear apparatus is a single centrally-placed nuclear group that consists of three large irregular macronuclei and 2-3 micronuclei; these are either clustered or encapsulated. 相似文献
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MARDER EVE; ABBOTT LAURENCE F.; BUCHHOLTZ FRANK; EPSTEIN IRVING R.; GOLOWASCH JORGE; HOOPER SCOTT L.; KEPLER THOMAS B. 《Integrative and comparative biology》1993,33(1):29-39
SYNOPSIS. The stomatogastric nervous system of decapod crustaceansis an ideal system for the study of the processes underlyingthe generation of rhythmic movements by the nervous system.In this chapter we review recent work that uses mathematicalanalyses and computer simulations to understand: 1) the roleof individual currents in controlling the activity of neurons,and 2) the effects of electrical coupling on the activity ofneuronal oscillators. The aim of this review is to highlight,for the physiologist, what these studies have taught us aboutthe organization and function of single cell and multicellularneuronal oscillators. 相似文献
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MARTIN CLÉMENT CAROLINE CHAMBERLAND JACQUELINE PÉRODIN RICHARD LEDUC GAÉTAN GUILLEMETTE EMANUEL ESCHER 《Journal of receptor and signal transduction research》2013,33(5-6):417-433
Several models of activation mechanisms were proposed for G protein-coupled receptors (GPCRs), yet no direct methods exist for their elucidation. The availability of constitutively active mutants has given an opportunity to study active receptor conformations within acceptable limits using models such as the angiotensin II type 1 (AT1)1 receptor mutant N111G-hAT1 which displays an important constitutive activity. Recently, by using methionine proximity assay, we showed for the hAT1 receptor that TMD III, VI, and VII form the ligand-binding pocket of the C-terminal amino acid of an antagonistic AngII analogue. In the present contribution, we investigated whether the same residues would also constitute the ligand-binding contacts in constitutively activated mutant (CAM) receptors. For this purpose, the same Met mutagenesis strategy was carried out on the N111G double mutants. Analysis of 43 receptors mutants in the N111G-hAT1 series, photolabeled and CNBr digested, showed that there were only subtle structural changes between the wt-receptor and its constitutively active form. 相似文献
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Abstract Possible effects of increased atmospheric concentrations of CO2 on forest ecosystems are discussed and as an example a simulation case study using a set of mixed-age and mixed-species forest stand models is presented. The responses of the models to a simple scenario (uniform growth increase of all trees as a response to CO2 enrichment) include increases in biomass that are considerably less than the increases in growth rate of the trees. These simulations and more general discussion of the possible effects of increased photosynthetic production identify the problem of scaling-up small time-scale and space-scale measurements of plant responses to CO2 enrichment to the ecosystem level. 相似文献
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