Phylogenetic and Functional Implications of the Rhabdom Patterns in the Eyes of Chrysomeloidea (Coleoptera)

Ultrastructural data from 108 species of Chrysomeloidea show that all rhabdom-patterns can be assigned to one of two basic patterns. The insula-pattern: two central rhabdomeres (Rh 7/8) are spatially isolated from the six peripheral ones (Rh 1–6). The ponticulus-pattern: Rh 7/8 fuse at two sites with the ring of Rh 1–6. The distance between the two systems may prevent optical or electrical coupling in the insula-p. The structure of the ponticulus-p may allow electrical coupling as well as contrast-intensifying lateral filtering. Potential relative polarization and absolute sensitivities differ interspecifically between homologous cells and intraspecifically between Rh7/8 and Rh 1–6, and between Rh 7 and Rh 8. The Bruchidae show only the insula-p, the Chrysomelidae and Cerambycidae both. The distribution of the two patterns is subfamily-specific within the Chrysomelidae, but not in the Cerambycidae. Identical patterns must have developed convergently within the Chrysomeloidea. Both basic patterns are subdivided in different subfamilies or tribes.     

Solute Leakage from the Isolated Parenchyma of Allium cepa and Kalanchoe daigremontiana

Efflux of different solutes from leaf slices of Kalanchoëdaigremontiana and from slices of the onion bulb scale was reinvestigatedwith respect to (1) dependence on turgor, (2) selectivity, (3)integrity of protoplasts and cellular changes. In both materials efflux of solutes (electrolytes or sugars)is non-selective and strongly dependent on turgor. Treatmentof tissue slices with hypotonic solutions (below a criticalosmotic pressure) resulted in high leakage rates, an increasein free space and an increased number of damaged cells. Lowconcentrations of calcium did not prevent this loss of retentionand cell stability. Part of the surviving cells were found to have a strongly decreasedosmotic pressure of cell sap. Leakage did not occur simultaneouslyat all cells of the tissue slice. It can be concluded that effluxfrom parenchyma cells in hypotonic solutions results from irreversibleosmotic breakdown and reversible membrane defects both favouredby high turgor. Key words: Parenchyma cells, Allium cepa, Kalanchoé daigremontiana, Solute efflux, Viability, Permeability, Plasmoptysis