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Kinetics of Integration of Xyloglucan into the Walls of Suspension-Cultured Rose Cells 总被引:1,自引:0,他引:1
To study the kinetics of synthesis, wall-binding and degradationof xyloglucan, we incubated suspension-cultured rose cells for0524 h in L-[1-3H]arabinose. >95% of the [3H]arabinosewas taken up within 2 h. UDP-Pentoses were maximally labelledwithin 05 h and had lost most of their 3H by 2 h afterthe addition of [3H]arabinose. Therefore, the 24 h experimentresembled a pulse-chase rgime. The [3H]xyloglucan formed wasfractionated into four cellular pools [detergent-extractable(interpreted as cytoplasmic), and guanidinium thiocyanate-,06 M NaOH- and 60 M NaOH-extractable (interpreted as progressivelymore firmly wall-bound)]; soluble extracellular xyloglucan wascollected as a fifth pool. All five pools of xyloglucan hadstarted accumulating 3H at their respective maximal rates by 相似文献
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