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Karina Pokusaeva Nomeda Kuisiene Dziuginta Jasinskyte Kazimiera Rutiene Jordana Saleikiene Donaldas Chitavichius 《Central European Journal of Biology》2009,4(2):196-203
Four novel heat-stable bacteriocin-like substances were found to be produced by Geobacillus stearothermophilus strains isolated from oil-wells in Lithuania. Geobacillus stearothermophilus 32A, 17, 30 and 31 strains were identified as producers of bacteriocins with bactericidal activity against closely related
Geobacillus species and several pathogenic strains: Bacillus cereus DSM 12001 and Staphylococcus haemolyticus P903. The secretion of the analysed bacteriocins started during early logarithmic growth and dropped sharply after the culture
entered the stationary phase of growth. The antimicrobial activity of the bacteriocins against sensitive indicator cells disappeared
after treatment with proteolytic enzymes, indicating their proteinaceous nature. Bacteriocins were stable throughout the pH
range between 4 and 10, and no loss in activity was noted following temperature exposures up to 100°C. Direct detection of
antibacterial activity on SDS-PAGE suggests that the inhibitory peptides have a molecular weight of 6–7.5 kDa. Such bacteriocins
with broad activity spectra, including antipathogenic action, are attractive to the biotechnology industry as they could be
used as antimicrobial agents in medicine, agriculture and food products. 相似文献
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