Mathematical Modeling of Open States in DNA Molecule Depending on the Deuterium Concentration in the Surrounding Liquid Media at Different Values of Hydrogen Bond Disruption Energy

The effect of the deuterium concentration in the surrounding liquid medium on the appearance of open states between the nitrogen bases of a double-stranded DNA molecule was investigated. It is shown that the nature of this effect depends on the magnitude of the energy of the hydrogen bond break.     

EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues

Using EPR spectroscopy it was established that the determination of the concentration of paramagnetic centers in lyophilized tissues allows indirect evaluation of the quality of decellularization of intrathoracic organs (diaphragm, heart, and lungs), since the content of paramagnetic particles in them can serve as a criterion of cell viability and points to the necessity to repeat decellularization. Experiments in rats showed that the EPR spectra of the native thoracic organs contained paramagnetic centers with g-factor values ranging from 2.007 to 2.011 at a concentration of 10^–8 to 6.62 × 10^–7 mol/g of lyophilized tissue, whereas in all decellularized tissues of the same organs paramagnetic particles were not detected.     

A Mathematical Model for Basepair Opening in a DNA Double Helix

In this paper, we consider a mathematical model that draws an analogy between a DNA molecule and a mechanical system consisting of two chains of interconnected pendulums. This model is designed to explore the dynamics of the system determined by rotational movements of nucleobases around a double-stranded pentose phosphate backbone. The workability of this model is assessed with respect to various factors: inhomogeneity of the chain of nucleobases, the properties of bonds in complementary pairs, and the formation of open states. It has been shown that simplified models for averaging the characteristics of the chain of nucleobases or simplification of the type of hydrogen bond in their complementary pairs influence the type of solution significantly, impairing the validity of the results. Therefore, the approach to the solution of rotational DNA molecule dynamics developed here is more consistent with its actual biomechanics. It is shown that the emergence of open states within nucleobase pairs and restoration of the closed structure may occur in the tested mathematical model.     

Influence of deuterium depleted water on freeze-dried tissue isotopic composition and morphofunctional body performance in rats of different generations

The influence of deuterium depleted water on the body of different rat generations was investigated in physiological conditions. As a result of this study it was established that the most significant and rapid reduction in D/H equilibrium was observed in plasma (by 36.2%), and lyophilized kidney tissues (by 15.8%). Less pronounced deuterium decrease was characteristic of liver tissue (9.3%) and heart (8.5%). Stabilization of the isotopic exchange reaction rate was fixed in the blood and tissues of rats, starting from the second generation. At the same time when deuterium depleted water (40 ppm) was used in dietary intake, the change in morphological and functional parameters in laboratory animals associated with the processes of adaptation to the effects of substress isotopic D/H gradient was also noted. The study shows that modification of only drinking water intake regime can’t significantly change the deuterium content in tissues of metabolically active organs, because of the concurrent deuterium receipt in feed substances of plant and animal origin.     

Sus Scrofa immune tissues as a new source of bioactive substances for skin wound healing

Influence of a new protein-peptide complex on promoting skin wound healing in male BALB/c mice was studied. Protein-peptide complex, extracted from Sus scrofa immune organs, was percutaneously administered using two methods: by lecithin gel-like liquid crystals and by liquid microemulsion. On the fifth day, wound closure in mice with a linear wound model become faster in group (less 2 days comparison to other ones), which was treated with lecithin liquid crystals carrying the protein-peptide complex. This promoting healing can be caused by resorption of bioactive high-molecular compounds the animal skin. In mice with the linear wound model, the tensile strength of the scars were respectively higher both in mice, treated using lecithin liquid crystals with protein-peptide complex, and in mice, treated using microemulsion containing protein-peptide complex, by 215.4% and 161.5% relative to the animals, which did not receive bioactive substances for wound treatment. It was associated with the regeneratory effects of tissue- and species-specific protein-peptide complexes, including α-thymosin Sus scrofa (C3VVV8_PIG, m/z 3802.8) and other factors, which were described as parts of the new extracted complex. This reveals that percutaneous administration of the complex reliably activates local regenerative processes in animals.     

Influence of deuterium depleted water on the organism of laboratory animals in various functional conditions of nonspecific protective systems

The influence of deuterium depleted water with the modified isotope composition on an organism of animals is studied in physiological conditions and development of chronic endogenous intoxication of hepatorenal genesis. The influence of this water on isotope composition of plasma and tissues (the liver and kidneys) in laboratory animals is shown. The impact of this water on biochemical indicators (AST, ALT, alkaline phosphatase, creatinine, bilirubin) and dynamics in body weight of laboratory animals within 42 days is established. As a result of studies the possibility of the preventive use of deuterium depleted water for correction of metabolic processes is shown in various conditions of the functional system of the body’s detoxification.     

The Mechanisms of the Interaction of Stable Isotopes with Biological Objects in the Presence of an Uncompensated Neutron in Chemical Bonds

The physical regularities that lead to isotope fractionation and to the accumulation of certain isotope forms in the intercellular and intracellular space at various levels of biological objects were studied. A new hypothesis of fractionation of stable isotopes in biological objects through the neutron effect was discussed. The following feature is noted: it is very likely that the development of isotope shock in living beings is observed mainly in the presence of covalent bonds between atoms with an uncompensated neutron. One possible explanation for this phenomenon can be associated with a change in the physical parameters of the following events: the interactions of the magnetic moments of valence electrons with the magnetic moments of atomic nuclei and the interactions of magnetic moments of atomic nuclei, which result in a change in the distance between atomic nuclei.

     

Content of deuterium in biological fluids and organs: Influence of deuterium depleted water on D/H gradient and the process of adaptation

It is found that consumption of deuterium depleted water reduces not only the content of deuterium in biological fluids but also more than 2 times reduces the D/H gradient value along the line: mixed saliva > blood plasma. The experimental data showed that a physiological solution prepared on deuterium depleted water during induced apoptosis activates the DNA repair system, significantly reducing the number of single-stranded DNA breaks, which, in general, indicates an increase in the efficiency of defensive systems of the cell.     

Review of Mathematical Models Describing the Mechanical Motion in a DNA Molecule

Biophysics - Several mechanical models that reproduce the DNA structure were considered. Evolution was described in more detail for the mechanical model proposed for a DNA molecule by Englander,...