Detection of Three-Nucleotide Deletion ΔF508 in Human Gene CFTR by UV Immobilization on Nylon of a Complex Formed by the PCR-Amplified DNA Fragment with a Highly Specific Probe

Deletion F508 has been revealed in PCR-amplified regions of human gene CFTR by color detection of the hybridization complex obtained by ligation of a tandem of short oligonucleotides on a DNA template followed by UV immobilization on nylon. The method allows reliable detection of the three-nucleotide deletion (insertion). The nonspecific signal depends on the nucleotide composition of the biotinylated tandem component. A significant level of the specific signal was achieved by using the PCR-amplified DNA fragments of different length (200–400 bp) irrespective of the position of the tandem-binding site in their sequences.