排序方式: 共有7条查询结果,搜索用时 15 毫秒
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Adluri RS Thirunavukkarasu M Zhan L Dunna NR Akita Y Selvaraju V Otani H Sanchez JA Ho YS Maulik N 《PloS one》2012,7(3):e34790
Oxidative stress plays a critical role in the pathophysiology of cardiac failure, including the modulation of neovascularization following myocardial infarction (MI). Redox molecules thioredoxin (Trx) and glutaredoxin (Grx) superfamilies actively maintain intracellular thiol-redox homeostasis by scavenging reactive oxygen species. Among these two superfamilies, the pro-angiogenic function of Trx-1 has been reported in chronic MI model whereas similar role of Grx-1 remains uncertain. The present study attempts to establish the role of Grx-1 in neovascularization and ventricular remodeling following MI. Wild-type (WT) and Grx-1 transgenic (Grx-1(Tg/+)) mice were randomized into wild-type sham (WTS), Grx-1(Tg/+) Sham (Grx-1(Tg/+)S), WTMI, Grx-1(Tg/+)MI. MI was induced by permanent occlusion of the LAD coronary artery. Sham groups underwent identical time-matched surgical procedures without LAD ligation. Significant increase in arteriolar density was observed 7 days (d) after surgical intervention in the Grx-1(Tg/+)MI group as compared to the WTMI animals. Further, improvement in myocardial functional parameters 30 d after MI was observed including decreased LVIDs, LVIDd, increased ejection fraction and, fractional shortening was also observed in the Grx-1(Tg/+)MI group as compared to the WTMI animals. Moreover, attenuation of oxidative stress and apoptotic cardiomyocytes was observed in the Grx-1(Tg/+)MI group as compared to the WTMI animals. Increased expression of p-Akt, VEGF, Ang-1, Bcl-2, survivin and DNA binding activity of NF-κB were observed in the Grx-1(Tg/+)MI group when compared to WTMI animals as revealed by Western blot analysis and Gel-shift analysis, respectively. These results are the first to demonstrate that Grx-1 induces angiogenesis and diminishes ventricular remodeling apparently through neovascularization mediated by Akt, VEGF, Ang-1 and NF-κB as well as Bcl-2 and survivin-mediated anti-apoptotic pathway in the infarcted myocardium. 相似文献
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Rajagopal Taruna Seshachalam Arun Rathnam Krishna Kumar Jothi Arunachalam Viswanathan Swarna Talluri Srikanth Dunna Nageswara Rao 《Molecular biology reports》2020,47(7):5081-5090
Molecular Biology Reports - Identification of modifier genes predisposing to breast cancer (BC) phenotype remains a significant challenge and varies with ethnicity. The genetic variability observed... 相似文献
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Dunna Vijay Malvika Dadlani Polumetla Ananda Kumar Siva Kumar Panguluri 《Plant Molecular Biology Reporter》2009,27(3):282-291
Storage of seeds for extended periods causes a number of degradative changes related to the aging process such as decreased
seedling vigor and reduced germination. In this study, molecular markers were used to study the aging process in seeds of
two different plants species. Seeds of three differentially aged seed groups, including control (un-aged), naturally aged,
and accelerated aging, from soybean (Glycine max) and safflower (Carthamus tinctorius) were evaluated for genetic variability using random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism
(AFLP), and simple sequence repeat (SSR) markers. For both plant species, naturally aged and accelerated aged groups clustered
together with RAPD markers, whereas control and naturally aged seeds showed similarity in both AFLP and SSR profiles. Based
on these findings, it can be concluded that observed changes in DNA profiles of seeds from different aged groups did not contribute
to accumulation of genetic variations of the same magnitude. Therefore, seed of similar viability must be selected for molecular
marker analysis for plant variety protection, among other comparative studies. 相似文献
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Tricarboxylic acid cycle and anaplerotic enzymes in rhizobia 总被引:8,自引:0,他引:8
Michael F Dunna 《FEMS microbiology reviews》1998,22(2):105-123
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