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Live Faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier 下载免费PDF全文
Dulantha Ulluwishewa Rachel C. Anderson Wayne Young Warren C. McNabb Peter van Baarlen Paul J. Moughan Jerry M. Wells Nicole C. Roy 《Cellular microbiology》2015,17(2):226-240
Faecalibacterium prausnitzii, an abundant member of the human commensal microbiota, has been proposed to have a protective role in the intestine. However, it is an obligate anaerobe, difficult to co‐culture in viable form with oxygen‐requiring intestinal cells. To overcome this limitation, a unique apical anaerobic model of the intestinal barrier, which enabled co‐culture of live obligate anaerobes with the human intestinal cell line Caco‐2, was developed. Caco‐2 cells remained viable and maintained an intact barrier for at least 12 h, consistent with gene expression data, which suggested Caco‐2 cells had adapted to survive in an oxygen‐reduced atmosphere. Live F. prausnitzii cells, but not ultraviolet (UV)‐killed F. prausnitzii, increased the permeability of mannitol across the epithelial barrier. Gene expression analysis showed inflammatory mediators to be expressed at lower amounts in Caco‐2 cells exposed to live F. prausnitzii than UV‐killed F. prausnitzii, This, consistent with previous reports, implies that live F. prausnitzii produces an anti‐inflammatory compound in the culture supernatant, demonstrating the value of a physiologically relevant co‐culture system that allows obligate anaerobic bacteria to remain viable. 相似文献
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Genome mapping of an apple scab,a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection 总被引:1,自引:0,他引:1
Vincent G. M. Bus Heather C. M. Bassett Deepa Bowatte David Chagné Chandra A. Ranatunga Dulantha Ulluwishewa Claudia Wiedow Susan E. Gardiner 《Tree Genetics & Genomes》2010,6(3):477-487
Apple is host to a wide range of pests and diseases, with several of these, such as apple scab, powdery mildew and woolly
apple aphid, being major causes of damage in most areas around the world. Resistance breeding is an effective way of controlling
pests and diseases, provided that the resistance is durable. As the gene pyramiding strategy for increasing durability requires
a sufficient supply of resistance genes with different modes of action, the identification and mapping of new resistance genes
is an ongoing process in breeding. In this paper, we describe the mapping of an apple scab, a powdery mildew and a woolly
apple aphid gene from progeny of open-pollinated mildew immune selection. The scab resistance gene Rvi16 was identified in progeny 93.051 G07-098 and mapped to linkage group 3 of apple. The mildew and woolly aphid genes were identified
in accession 93.051 G02-054. The woolly aphid resistance gene Er4 mapped to linkage group 7 to a region close to where previously the genes Sd1 and Sd2, for resistance to the rosy apple leaf-curling aphid, had been mapped. The mildew resistance gene Pl-m mapped to the same region on linkage group 11 where Pl2 had been mapped previously. Flanking markers useful for marker-assisted selection have been identified for each gene. 相似文献
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