全文获取类型
收费全文 | 898篇 |
免费 | 92篇 |
专业分类
990篇 |
出版年
2021年 | 13篇 |
2020年 | 12篇 |
2019年 | 13篇 |
2018年 | 15篇 |
2017年 | 23篇 |
2016年 | 10篇 |
2015年 | 28篇 |
2014年 | 31篇 |
2013年 | 43篇 |
2012年 | 55篇 |
2011年 | 64篇 |
2010年 | 35篇 |
2009年 | 35篇 |
2008年 | 41篇 |
2007年 | 47篇 |
2006年 | 43篇 |
2005年 | 45篇 |
2004年 | 32篇 |
2003年 | 38篇 |
2002年 | 39篇 |
2001年 | 34篇 |
2000年 | 35篇 |
1999年 | 26篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1992年 | 16篇 |
1991年 | 6篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 12篇 |
1987年 | 14篇 |
1986年 | 11篇 |
1985年 | 7篇 |
1984年 | 3篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 8篇 |
1976年 | 4篇 |
1975年 | 7篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1970年 | 5篇 |
1968年 | 3篇 |
1966年 | 6篇 |
排序方式: 共有990条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
E P Geiduschek M C Armelin R Petrusek C Bread J J Duffy G Johnson 《Journal of molecular biology》1977,117(4):825-842
The uptake of a homologous single-stranded fragment by superhelical DNA produces a complex that contains a stable displacement loop. When the circular DNA was relaxed by the random action of pancreatic DNAase, complexes dissociated by a process which requires that the single-stranded arm of the D-loop be intact. We attribute the dissociation to branch migration, the exchange of like strands at a branch point. The kinetics of dissociation were biphasic. A fraction of the nicked complexes dissociated in a few seconds, the rest dissociated much more slowly. The fraction of molecules that dissociated slowly was directly related to the length of the third strand, and inversely related to temperature. Salt also inhibited dissociation. Under physiological conditions, 37 °C and 0.15 m-NaCl, more than half of complexes containing a third strand of 1000-nucleotide residues survived for at least one minute. These observations provide a guide to handling certain natural or synthetic branched derivatives of DNA. Analyzing our data by the method of Thompson et al. (1976), we have estimated that the time for the exchange of one nucleotide for another at a single-stranded branch is 12 microseconds; but the calculated value depends strongly upon the assumption that single-strand branch migration occurs by a random walk. 相似文献
5.
CARBOHYDRATE AND ENERGY METABOLISM IN PERINATAL RAT BRAIN: RELATION TO SURVIVAL IN ANOXIA 总被引:18,自引:8,他引:10
The ability of rats of different ages to survive exposure to anoxia was correlated with rates of high energy phosphate consumption (metabolic rates) of the fore-brain. Fetal rats at term, delivered by hysterotomy following maternal decapitation, survived in nitrogen at 37°C twice as long as 1-day-old neo-nates, 5 times longer than 7-day-old rats, and 45 times longer than adults. During ischemia induced by decapitation, the cerebral concentrations of the labile energy reserves (ATP, ADP, P-creatine, glucose and glycogen) and of lactate were determined in fetuses, 1- and 7-day post-natal animals. From the changes, the cerebral energy use rates were calculated to be 1·57 mmol/kg/min in fetuses, 1·33 mmol/kg/min in 1-day-olds and 2·58 mmol/kg/min in 7-day-olds. Maximal rates of lactate accumulation during ischemia, as a measure of glycolytic capacity, were comparable in fetuses and neonates, but were about twice as great in 7-day-old rats. It is concluded that in post-natal animals survival in anoxia and cerebral energy consumption are inversely, and nearly quantitatively, related. However, the reduced cerebral energy requirement cannot entirely account for the greater anoxic resistance of fetuses. 相似文献
6.
K E Schwartz B Zaro J Reynolds J Duffy T Saito J S Hunt H Sevelius 《Hormones et métabolisme》1988,20(10):637-640
Enprostil, a long-acting, orally active dehydroprostaglandin E2 with cytoprotective and gastric antisecretory properties, is a potent inhibitor of meal-stimulated gastrin release. Recent data have suggested suppression of additional other gastrointestinal peptide hormones following single doses of enprostil. The current investigation was conducted to further clarify the effects of enprostil administration on gastrointestinal hormones and glucose metabolism under physiologic conditions and to determine whether these effects were present following multiple doses of the agent. Enprostil 70 mcg/d and its placebo were each administered for 7 1/2 days to eight normal male subjects in a study of crossover design, each treatment period lasting 7 1/2 days and separated by a 7 day washout period. Subjects received a test meal on days 1 and 8 and an oral glucose challenge on day 3 of each treatment period following enprostil or its placebo. Following the test meal, there was a delay and suppression of the maximum measured serum glucose levels. Mean overall peak glucose concentrations were lower during the enprostil phase compared to placebo (112 vs. 121 mg/dd, P = 0.025) with a trend toward delay in the time to achievement of peak glucose concentrations. Mean overall peak levels for insulin, C-peptide, and glucose-dependent insulinotropic peptide (GIP) were significantly suppressed by 36%, 16% and 60%, respectively by enprostil when compared to placebo. The overall integrated postprandial responses for insulin, C-peptide, and GIP were significantly reduced by 42%, 39% and 90%, respectively while that for glucose above baseline was reduced by 44% (P = 0.098). Similar effects were present following the oral glucose challenge.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
7.
Samples of tongue or diaphragm from 2,056 black bears harvested in Pennsylvania during the 1981-1983 hunting seasons were examined for larvae of Trichinella spiralis by peptic digestion. Sixteen males and 21 females were infected. The overall prevalence of infection was 1.8%. Infected animals were distributed widely throughout the range of the bear in Pennsylvania. In samples from infected bears, the geometric mean density of muscle larvae was 7.8 per g of tissue (LPG). There were neither sex- nor age-related differences in prevalence or intensity of infection. Virtually all bears harvested in Pennsylvania are consumed as food, which often is shared widely among hunters, their friends and relatives. Furthermore, high densities of larvae occurred in some bears (i.e., 300, 348, 465, 512, 555, and 912 LPG). Thus, a basis for potential, single-source outbreaks of severe human trichinosis exists. 相似文献
8.
9.
10.
Gel retardation assays using a probe containing the repeat region of a Schizosaccharomyces pombe chromosomal telomere identified four specific DNA- protein complexes in S. pombe total protein extracts (I, I', IIa and IIb). The proteins responsible for these complexes bound to the telomeric repeat region irrespective of whether or not the repeats were in close proximity to the end of a DNA molecule, and none of them bound strongly to single-stranded DNA. The protein responsible for complex I (TeRF I) was separated from the activity responsible for complexes IIa and IIb (TeRF II) using heparin-Sepharose chromatography. Both factors were efficiently cross-competed by an oligonucleotide containing the 18 bp sequence 5'-GGTTACAGGTTACAGGTT-3', which corresponds to two complete telomeric repeat units. Mutation of the T residues at positions 4 and 11 in the oligonucleotide dramatically reduced binding by TeRF II, but had no affect on binding by TeRF I. The protein responsible for complex I' did not bind strongly to either the wild-type or mutant oligonucleotide. 相似文献