The Generation of Superoxide Radicals by Cardiac Mitochondria and the Antioxidant Effect of the Water-Soluble Form of Ubiquinol-10

Biophysics - We have studied the effect of the water-soluble form of ubiquinol-10 (CoQ10-H2) on the processes of electron transport, oxidative phosphorylation, and the formation of reactive oxygen...     

The generation of superoxide radicals by complex III in heart mitochondria and the antioxidant effect of dinitrosyl iron complexes at different partial pressures of oxygen

EPR spin trapping and EPR oximetry were used to study the superoxide radical generation in heart mitochondria from Wistar rats at various oxygen concentrations. Lithium phthalocyanine and TEMPONE-¹⁵N-D₁₆ were chosen to determine the oxygen content in a gas-permeable capillary containing mitochondria. TIRON was used as a spin trap. Several oxygen concentrations in the incubation mixture were tested; heart mitochondria were found to generate superoxide in complex III at various partial pressures of oxygen, including deep hypoxia (<5% O₂). Dinitrosyl iron complexes with glutathione (the drug Oxacom) exerted an antioxidant effect regardless of the partial pressure of oxygen; the magnitude and kinetic characteristics of the effect depended on the drug concentration.     

Generation of Superoxide Radicals by Heart Mitochondria and the Effects of Dinitrosyl Iron Complexes and Ferritin

Biophysics - Mitochondrial dysfunction, which may be due to excessive generation of toxic forms of oxygen and nitrogen in oxidative stress, is one of the risk factors for a wide range of...     

The Physicochemical Characteristics of Serum Albumin and Erythrocyte Cell Membranes under Normal and Heart Failure Symptom Conditions

Biophysics - Abstract—Spin labeling EPR spectroscopy was used to study the structural and conformational characteristics of human serum albumin and human erythrocyte membranes under normal...