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排序方式: 共有141条查询结果,搜索用时 15 毫秒
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Bogolitsyn K. G. Parshina A. E. Druzhinina A. S. Shulgina E. V. 《Russian Journal of Bioorganic Chemistry》2021,47(7):1395-1403
Russian Journal of Bioorganic Chemistry - Brown algae represent a valuable source of various biologically active compounds. Their accumulation is influenced by many environmental factors. The... 相似文献
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Species of the mitosporic filamentous fungal genus Trichoderma are prominent producers of both short (7-11 residues) and long (18-20 residues) peptaibols and peptaibiotics, which are thought to be involved in their interaction with other living systems. Numerous reviews are available regarding biodiversity, structure, and mode of action of these peptide derivatives, but little emphasis has been paid to the physiology and genetics of their formation. In this review article, we used the recent knowledge on biosynthesis and production of these components to speculate on some of the unknown points. We also highlight areas where further research is most urgently needed. 相似文献
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Carlos A. López-Quintero Lea Atanasova A. Esperanza Franco-Molano Walter Gams Monika Komon-Zelazowska Bart Theelen Wally H. Müller Teun Boekhout Irina Druzhinina 《Antonie van Leeuwenhoek》2013,104(5):657-674
The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution. 相似文献
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Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation 总被引:1,自引:0,他引:1
We have identified a new gene, Zfrp8, as being essential for hematopoiesis in Drosophila. Zfrp8 (Zinc finger protein RP-8) is the Drosophila ortholog of the PDCD2 (programmed cell death 2) protein of unknown function, and is highly conserved in all eukaryotes. Zfrp8 mutants present a developmental delay, lethality during larval and pupal stages and hyperplasia of the hematopoietic organ, the lymph gland. This overgrowth results from an increase in proliferation of undifferentiated hemocytes throughout development and is accompanied by abnormal differentiation of hemocytes. Furthermore, the subcellular distribution of gamma-Tubulin and Cyclin B is affected. Consistent with this, the phenotype of the lymph gland of Zfpr8 heterozygous mutants is dominantly enhanced by the l(1)dd4 gene encoding Dgrip91, which is involved in anchoring gamma-Tubulin to the centrosome. The overgrowth phenotype is also enhanced by a mutation in Cdc27, which encodes a component of the anaphase-promoting complex (APC) that regulates the degradation of cyclins. No evidence for an apoptotic function of Zfrp8 was found. Based on the phenotype, genetic interactions and subcellular localization of Zfrp8, we propose that the protein is involved in the regulation of cell proliferation from embryonic stages onward, through the function of the centrosome, and regulates the level and localization of cell-cycle components. The overproliferation of cells in the lymph gland results in abnormal hemocyte differentiation. 相似文献
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Brandstaetter Christian Fricko Nora Rahimi Mohammad J. Fellner Johann Ecker-Lala Wolfgang Druzhinina Irina S. 《Biodegradation》2022,33(1):71-85
Biodegradation - Biological waste degradation is the main driving factor for landfill emissions. In a 2-year laboratory experiment simulating different landfill in-situ aeration scenarios, the... 相似文献
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Doris Ribitsch Enrique Herrero Acero Agnieszka Przylucka Sabine Zitzenbacher Annemarie Marold Caroline Gamerith Rupert Tschelie?nig Alois Jungbauer Harald Rennhofer Helga Lichtenegger Heinz Amenitsch Klaus Bonazza Christian P. Kubicek Irina S. Druzhinina Georg M. Guebitz 《Applied and environmental microbiology》2015,81(11):3586-3592
Cutinases have shown potential for hydrolysis of the recalcitrant synthetic polymer polyethylene terephthalate (PET). We have shown previously that the rate of this hydrolysis can be enhanced by the addition of hydrophobins, small fungal proteins that can alter the physicochemical properties of surfaces. Here we have investigated whether the PET-hydrolyzing activity of a bacterial cutinase from Thermobifida cellulosilytica (Thc_Cut1) would be further enhanced by fusion to one of three Trichoderma hydrophobins, i.e., the class II hydrophobins HFB4 and HFB7 and the pseudo-class I hydrophobin HFB9b. The fusion enzymes exhibited decreased kcat values on soluble substrates (p-nitrophenyl acetate and p-nitrophenyl butyrate) and strongly decreased the hydrophilicity of glass but caused only small changes in the hydrophobicity of PET. When the enzyme was fused to HFB4 or HFB7, the hydrolysis of PET was enhanced >16-fold over the level with the free enzyme, while a mixture of the enzyme and the hydrophobins led only to a 4-fold increase at most. Fusion with the non-class II hydrophobin HFB9b did not increase the rate of hydrolysis over that of the enzyme-hydrophobin mixture, but HFB9b performed best when PET was preincubated with the hydrophobins before enzyme treatment. The pattern of hydrolysis by the fusion enzymes differed from that of Thc_Cut1 as the concentration of the product mono(2-hydroxyethyl) terephthalate relative to that of the main product, terephthalic acid, increased. Small-angle X-ray scattering (SAXS) analysis revealed an increased scattering contrast of the fusion proteins over that of the free proteins, suggesting a change in conformation or enhanced protein aggregation. Our data show that the level of hydrolysis of PET by cutinase can be significantly increased by fusion to hydrophobins. The data further suggest that this likely involves binding of the hydrophobins to the cutinase and changes in the conformation of its active center. 相似文献
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The holomorph of the new species Hypocrea voglmayrii (Hypocreales, Ascomycota, Fungi) is described by a combined approach, using morphology of the teleomorph, morphology of the anamorph, culture studies and phylogenetic analyses of ITS1 and 2, ech42 and rpb2 gene sequences. Its anamorph Trichoderma voglmayrii is described as a new anamorph species. Unlike most other species of Hypocrea the teleomorph of H. voglmayrii occurs on dry standing trunks and exhibits well defined black ostioles. Although exclusively collected at higher altitudes, this species grows at 35 C in culture. Hypocrea voglmayrii develops pale yellowish to greenish conidia, a yellowish pigment and a coconut-like odor on CMD. Phylogenetically, H. voglmayrii forms a distinct, isolated branch between the section Trichoderma and the H. pachybasioides clade but does not associate with any of these clades in different gene trees. 相似文献
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Svetlana?Minakhina Ron?Myers Marina?Druzhinina Ruth?StewardEmail author 《BMC cell biology》2005,6(1):32