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1.
2.
Pampatheres are extinct, large‐bodied cingulates, which share morphological characters with both armadillos and glyptodonts but are considered to be more closely related to the latter. The osteoderm histology of six pampathere taxa was examined and compared to the histology of other cingulate osteoderms. This study investigates the development and functional adaptation of pampathere osteoderms as well as the phylogenetic relationships of the Pampatheriidae within the Cingulata. We found that pampathere osteoderms share a uniform histological organization based on a basic diploe‐like structure. After initial stages of intramembranous growth, metaplastic ossification, that is, the direct incorporation and mineralization of pre‐existing protein fibers, plays an important role in osteoderm development and provides information on various kinds of soft tissue otherwise not preserved. The latest stages of osteoderm growth are dominated by periosteal bone formation especially in the superficial cortex. Movable band osteoderms show regular arrangements of incorporated fibers that may increase the resistance of particularly weak areas against strain. The histological composition of pampathere osteoderms is plesiomorphic in its basic structure but shows a number of derived features. A unique array of Sharpey's fibers that are incorporated into the bone matrix at sutured osteoderm margins is interpreted as a synapomorphy of pampatheres. The arrangement of dermal fibers in the deep and superficial cortexes supports the close relationship between pampatheres and glyptodonts. J. Morphol., 2012. © 2011 Wiley Periodicals, Inc. 相似文献
3.
Christopher J. Bockisch Elham Khojasteh Dominik Straumann Stefan C. A. Hegemann 《PloS one》2012,7(12)
The nystagmus in patients with vestibular disorders often has an eye position dependency, called Alexander’s law, where the slow phase velocity is higher with gaze in the fast phase direction compared with gaze in the slow phase direction. Alexander’s law has been hypothesized to arise either due to adaptive changes in the velocity-to-position neural integrator, or as a consequence of processing of the vestibular-ocular reflex. We tested whether Alexander’s law arises only as a consequence of non-physiologic vestibular stimulation. We measured the time course of the development of Alexander’s law in healthy humans with nystagmus caused by three types of caloric vestibular stimulation: cold (unilateral inhibition), warm (unilateral excitation), and simultaneous bilateral bithermal (one side cold, the other warm) stimulation, mimicking the normal push-pull pattern of vestibular stimulation. Alexander’s law, measured as a negative slope of the velocity versus position curve, was observed in all conditions. A reversed pattern of eye position dependency (positive slope) was found <10% of the time. The slope often changed with nystagmus velocity (cross-correlation of nystagmus speed and slope was significant in 50% of cases), and the average lag of the slope with the speed was not significantly different from zero. Our results do not support the hypothesis that Alexander’s law can only be observed with non-physiologic vestibular stimulation. Further, the rapid development of Alexander’s law, while possible for an adaptive mechanism, is nonetheless quite fast compared to most other ocular motor adaptations. These results suggest that Alexander’s law may not be a consequence of a true adaptive mechanism. 相似文献
4.
Karl H. Summer Dominik Klein Nada de Ruiter Josef Abel 《Biological trace element research》1989,21(1):165-169
In the present study we report on the effects of commonly used nonsteroidal antiinflammatory drugs on metallothionein (MT)
and MT-I mRNA levels. A single dose of chloroquine (100 mg/kg), diclofenac (100 mg/kg), indomethacin (10 mg/kg), or piroxicam
(100 mg/kg) was administered ip to C57B1 mice. After 18 h, MT levels were determined with a Cd-saturation radioassay. MT-I
mRNA levels were measured by Northern Blot analyses using a probe containing the mouse MT-I gene. All drugs tested caused
an increase in the MT content of the liver but not of the kidneys and lung. The lowest and highest effects were observed with
chloroquine (8 times the control value) and diclofenac (18 times), respectively. In accordance with the stimulation of MT
synthesis, increased accumulation of hepatic MT-I mRNA could be demonstrated.
These results indicate that elevated MT levels may contribute to the effectiveness of nonsteroidal antiinflammatory drugs
in the treatment of rheumatoid arthritis (RA). 相似文献
5.
Gerard Strecker Jean-Michel Wieruszeski Jean-Claude Michalski Jean Montreuil 《Glycoconjugate journal》1988,5(4):385-396
The structure of a new nonasaccharide isolated from human milk has been investigated. By using methylation analysis, FAB-MS and1H-and13C-NMR spectroscopy as basic methods of structural investigation, this oligosaccharide was identified as VI2--Fuc,V4-Fuc,III3--Fuc-p-lacto-n-hexaose: Fuc1-2Gal1-3[Fuc1-4]GlcNAc1-3Gal1-4[Fuc1-3]GlcNAc1-3Gal1-4Glc.Abbreviations COSY
correlation spectroscope
- DP
degree of polymerisation
- FAB-MS
fast atom bombardment-mass spectrometry
- HPLC
high performance liquid chromatography
- NMR
nuclear magnetic resonance
- GLC
gas-liquid chromatography 相似文献
6.
The time delay of oxygen probe response to the signal from a fermenter makes identification of the volumetric oxygen transfer coefficient kLa by the dynamic method more complicated. A coupled model involving the transient-state oxygen balance of the fermenter together with the dynamic model of the oxygen probe must be then formulated, solved and identified. In this paper two simple models of air-lift loop fermenters have been proposed and a coupled mathematical model of the fermenter – oxygen probe system has been developed. The identification procedure was used to estimate kLa values in the fermenter with internal circulation flow on the basis of experimental measurements. A comparison of evaluated and experimental indications of the probes placed at various heights of the column proves that the model presented gives a possibility of the first-step approximation of kLa in loop fermenters. 相似文献
7.
T Baussant G Strecker J M Wieruszeski J Montreuil J C Michalski 《European journal of biochemistry》1986,159(2):381-385
An endo-N-acetyl-beta-D-glucosaminidase active towards oligosaccharides with a reducing terminal [bis(N-acetylglucosamine)]residue has been characterized in rat liver. The primary structure of its reaction products was determined using high-resolution 1H-NMR spectroscopy. The enzyme is predominantly located in the lysosomal fraction, presents a maximum of activity at pH 3.5 and is completely inactive towards conjugated glycans, i.e. glycoproteins and glycopeptides as well as on glycoasparagines. These results support the existence of a new pathway for the degradation of glycoprotein glycans inside the lysosome. In particular, this enzymic activity may be the origin of oligosaccharides bearing a single terminal reducing N-acetylglucosamine residue which are excreted in the urine of patients with various exoglycosidase deficiencies. 相似文献
8.
J C Michalski J Montreuil G Strecker H van Halbeek L Dorland J F Vliegenthart B Cartigny J P Farriaux 《European journal of biochemistry》1983,132(2):375-381
Initial-rate measurements were made of the reduction of pyridine-3-aldehyde and p-carboxybenzaldehyde by NADPH catalyzed by pig liver aldehyde reductase I. The initial velocity analysis and product inhibition data suggest that aldehyde reductase I obeys a compulsory-order mechanism with pyridine-3-aldehyde as substrate but follows a partially random-order pathway with p-carboxybenzaldehyde. The partially random-order pathway would be operative only at high concentrations of p-carboxybenzaldehyde. In both cases, aldehydes and the corresponding alcohol substrates inhibit the enzyme at high concentration. Abortive ternary complexes are shown to be formed with pyridine-3-aldehyde and with p-carboxybenzaldehyde. Dissociation of the coenzyme from the abortive ternary complex seems only to be observed with p-carboxybenzaldehyde. This study suggests overall that an enzyme kinetic mechanism may be different, depending on whether specific interactions can occur between certain amino acid residue(s) of the protein active site and substrates. Finally, the mechanism of the inhibition of pyridine-3-aldehyde reduction by diacid derivatives is discussed. 相似文献
9.
Abstract: Changes of the main hydrodynamic and oxygen transfer parameters during Aspergillus niger cultivation in an external-loop air-lift bioreactor of 200 dm3 operating capacity were investigated. The final average concentrations of biomass and citric acid obtained in batch fermentations were about 17 g 1-1 and 90 g 1-1 , respectively. Significant influence of the increasing biomass concentration on the rheological properties of the broth and operating parameters was found. Volumetric oxygen transfer coefficient. k L a , was found to be dependent on the apparent viscosity of the broth with an exponent of -0.984. 相似文献
10.
Martin Dominik Vollmer Karin Stadler-Fritzsche Michael Schlömann 《Archives of microbiology》1993,159(2):182-188
2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funnelled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.Abbreviations 2,4-D
2,4-dichlorophenoxyacetate 相似文献