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Antonelli A Fallahi P Ferrari SM Di Domenicantonio A Moreno M Lanni A Goglia F 《Journal of biological regulators and homeostatic agents》2011,25(4):655-660
Recently, it was demonstrated that 3,5-diiodo-L-thyronine (T2) stimulates the resting metabolic rate (RMR), and reduces body-weight gain of rats receiving a high-fat diet. The aim of this study is to examine the effects of chronic T2 administration on basal metabolic rate and body weight in humans. Two euthyroid subjects volunteered to undergo T2 administration. Body weight, body mass index, blood pressure, heart rate, electrocardiogram, thyroid and liver ultrasonography, glycemia, total cholesterol, triglycerides, free T3 (FT3), free T4 (FT4), T2, thyroid stimulating hormone (TSH) and RMR were evaluated at baseline and at the end of treatment. RMR increased significantly in each subject. After continuing the T2 treatment for a further 3 weeks (at 300 mcg/day), body weight was reduced significantly (p<0.05) (about 4 percent), while the serum levels of FT3, FT4 and TSH, were unchanged. No side effects were observed at the cardiac level in either subject. No significant change was observed in the same subjects taking placebo. 相似文献
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Gianni Bellocchi Marzia De Giacomo Nicoletta Foti Marco Mazzara Eleonora Palmaccio Cristian Savini Chiara Di Domenicantonio Roberta Onori Guy Van den Eede 《BMC biotechnology》2010,10(1):55
Background
The modular approach to analysis of genetically modified organisms (GMOs) relies on the independence of the modules combined (i.e. DNA extraction and GM quantification). The validity of this assumption has to be proved on the basis of specific performance criteria. 相似文献3.
E Santavenere A Cataldi R Rana M Vitale R Lisio L Di Domenicantonio L Zamai O Trubiani S Miscia 《Cell biology international reports》1991,15(12):1235-1242
The effects of r-TNF alpha on cell cycle progression and DNA polymerase activity in Daudi lymphoma cells have been analyzed. Cytofluorimetric analysis of the cell cycle after 6 to 24 hr of treatment revealed both a decrease of BrdU incorporation per cell and a light inhibition of S phase as assessed by the analysis of the percentual distribution of cell cycle compartments. The reduction of BrdU incorporation can be related to the early decrease in the rate of DNA synthesis that follows r-TNF alpha treatment. These results suggest that one of the early events induced by r-TNF alpha at nuclear level is the slowering of DNA synthesis leading to a reduced cell cycle progression. 相似文献
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Antonelli A Ferrari SM Fallahi P Piaggi S Di Domenicantonio A Galleri D Santarpia L Basolo F Ferrannini E Miccoli P 《Cytokine》2012,59(2):218-222
Until now, no data are present in literature about the prototype Th1 chemokine (C-X-C motif) ligand 10 (CXCL10) in anaplastic thyroid cancer (ATC). This study aimed to test in "primary human ATC cells" (ANA) vs "normal thyroid follicular cells" (TFC): (a) CXCL10 secretion basally and after interferon (IFN)-γ and/or tumor necrosis factor (TNF)-α stimulation; (b) peroxisome proliferator-activated receptor (PPAR)-γ activation by thiazolidinediones, rosiglitazone or pioglitazone, on CXCL10 secretion, on proliferation and apoptosis in ANA. We demonstrate that: (a) ANA, but not TFC, produced basally CXCL10, and did so in half of cases; (b) IFN-γ stimulated dose-dependently CXCL10, in ANA and TFC; (c) TNF-α did not induce CXCL10 secretion, in ANA and TFC; (d) IFN-γ+TNF-α induced a synergistic but variable release of CXCL10 in the different ANA preparations, while it was more reproducible in TFC; (e) rosiglitazone action on CXCL10 in ANA was inhibitory in 2/6, stimulatory in 1/6 and nil in 3/6, whereas it was inhibitory in TFC; (f) rosiglitazone inhibition of proliferation in ANA was not associated with the effect on CXCL10; (g) nuclear factor-κB and ERK1/2 were basally activated in ANA, increased by IFN-γ+TNF-α, and rosiglitazone inhibited that activation. On the whole, the present data first show that ANA cells are able to produce CXCL10, basally and under the influence of cytokines. However, the pattern of modulation by IFN-γ, TNF-α or thiazolidinediones is extremely variable, suggesting that the intracellular pathways involved in the chemokine modulation in ATC have different types of deregulation. 相似文献
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Alcaro S Scipione L Ortuso F Posca S Rispoli V Rotiroti D 《Bioorganic & medicinal chemistry letters》2002,12(20):2899-2905
Pivaloyl-choline iodide 1 interactions with acetylcholinesterase (AChE) have been studied by theoretical and enzymatic methods. An integrated computational approach has clearly shown a substrate rather than inhibitory profile for 1. Enzymatic experiments have also supported the same theoretical conclusion indicating that AChE was able to hydrolyze 1 to choline. 相似文献
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Since September 1997 was detected the presence of the Asian Tiger Mosquito (Aedes albopictus) in the peripheral areas of the city of Rome, the Environment Department has put in a strategy to combat and control the spread of this insect throughout the city, collaboration with the Istituto Superiore di Sanità (ISS) to aspects of study and monitoring of the phenomenon and with the Azienda Municipale Ambiente (AMA) for actions in the urban environment. In 1998 began the first contrast campaign in the town territory. The data coming from ISS are processed through a geographical territorial system (GIS) that allows real-time locating the degree of infestation and effectiveness of interventions, allowing the display of trends over time and the development of plans of action in urban territory. In parallel to this methodology operational, the Municipality of Rome has put in an information campaign designed to involve citizens in the fight against this insect. Today the situation in the city is under control, in case of emergency due to the spread of the virus Chikungunya is possible identify in advance the areas at greatest risk of infestation. Using this methodology work has enabled to contain operating costs and minimize the environmental impact by limiting interventions only to areas found positive. 相似文献
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Bulotta S Cerullo A Barsacchi R De Palma C Rotiroti D Clementi E Borgese N 《Experimental cell research》2006,312(6):877-889
The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertainties on its intracellular distribution. Here, we applied high resolution confocal microscopy to investigate the surface distribution of eNOS in transfected HeLa cells and in human umbilical vein endothelial cells (HUVEC) endogenously expressing the enzyme. In confluent and non-confluent HUVEC and HeLa cells, we failed to detect substantial colocalization between eNOS and caveolin-1 at the cell surface. Instead, in non-confluent cells, eNOS was concentrated in ruffles and at the leading edge of migrating cells, colocalizing with actin filaments and with the raft marker ganglioside G(M1), and well segregated from caveolin-1, which was restricted to the posterior region of the cells. Treatments that disrupted microfilaments caused loss of eNOS from the cell surface and decreased Ca(2+)-stimulated activity, suggesting a role of the cytoskeleton in the localization and function of the enzyme. Our results provide a morphological correlate for the role of eNOS in cell migration and raise questions on the site of interaction between eNOS and caveolin-1. 相似文献
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