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排序方式: 共有184条查询结果,搜索用时 15 毫秒
1.
V A Kashkin N A Patkina A Ia Bagrov Ia Iu Bagrov E E Zvartau 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2001,87(5):665-669
Intraperitoneal administration of marinobufagenin resulted in a reliable and dose-dependent suppression of ethanol self-administration in drug- and experimentally naive DBA/2 mice. The findings suggest that Na/K-ATPase contributes to both mediation of the ethanol reinforcing properties and the mood regulation. 相似文献
2.
Bogdanova Alexandra Sergeevna Sokolova Anastasiia Ivanovna Pavlova Elizaveta Robertovna Klinov Dmitry Vladimirovich Bagrov Dmitry Vladimirovich 《Journal of biological physics》2021,47(2):205-214
Journal of Biological Physics - The morphology and proliferation of eukaryotic cells depend on their microenvironment. When electrospun mats are used as tissue engineering scaffolds, the local... 相似文献
3.
Toropova Ya. G. Gorshkova M. N. Motorina D. S. Korolev D. V. Skorik Yu. A. Shulmeister G. A. Podyacheva E. Yu. Bagrov A. Ya. 《Journal of Evolutionary Biochemistry and Physiology》2021,57(4):782-791
Journal of Evolutionary Biochemistry and Physiology - Among the available nanoparticles, those based on iron oxide are of particular interest due to their biological safety, magnetic properties,... 相似文献
4.
Zakhar O. Shenkarev Mikhail A. Shulepko Maxim L. Bychkov Dmitrii S. Kulbatskii Olga V. Shlepova Nathalia A. Vasilyeva Alexander A. Andreev-Andrievskiy Anfisa S. Popova Evgeniya A. Lagereva Eugene V. Loktyushov Sergey G. Koshelev Morten S. Thomsen Dmitry A. Dolgikh Sergey A. Kozlov Pavel M. Balaban Mikhail P. Kirpichnikov Ekaterina N. Lyukmanova 《Journal of neurochemistry》2020,155(1):45-61
5.
Alexander V. Lukashin Dmitrii B. Beglov Maxim D. Frank-Kamenetskii 《Journal of biomolecular structure & dynamics》2013,31(3):517-523
Abstract In order to allow for real dielectric properties of a solvent in calculating of electrostatic characteristics of strongly charged polyions such as DNA in salt solution we consider a simple model of linear dielectric response of a medium. The interactions between charged particles are treated in the framework of self-consistent-field approximation. The basic characteristic of the problem, electrostatic potential, can be found from the solution of non-linear integro-differential equation. Specifically we consider so-called quasimacroscopic model where dielectric response of a medium depends only on the distance from the polyion. Application of the approach for calculating of the B-to-Z free energy qualitatively retains the main conclusion obtained previously within the model with fixed dielectric constant: non-monotonous behavior of the free energy difference as a function of ionic strength. At the same time, essential sensitivity of the results to specific values of dielectric parameters is observed. 相似文献
6.
7.
Alexander M. Matyushenko Daniil V. Shchepkin Denis S. Susorov Victoria V. Nefedova Galina V. Kopylova Valentina Y. Berg Sergey Y. Kleymenov Dmitrii I. Levitsky 《Biochemical and biophysical research communications》2019,508(3):934-939
Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein that plays a key role in the Ca2+-regulated contraction of striated muscles. Two Tpm isoforms, α (Tpm 1.1) and β (Tpm 2.2), are expressed in fast skeletal muscles. These Tpm isoforms can form either αα and ββ homodimers, or αβ heterodimers. However, only αα-Tpm and αβ-Tpm dimers are usually present in most of fast skeletal muscles, because ββ-homodimers are relatively unstable and cannot exist under physiologic conditions. Nevertheless, the most of previous studies of myopathy-causing mutations in the Tpm β-chains were performed on the ββ-homodimers. In the present work, we applied different methods to investigate the effects of two myopathic mutations in the β-chain, Q147P and K49del (i.e. deletion of Lys49), on structural and functional properties of Tpm αβ-heterodimers and to compare them with the properties of ββ-homodimers carrying these mutations in both β-chains. The results show that the properties of αβ-Tpm heterodimers with these mutations in the β-chain differ significantly from the properties of ββ-homodimers with the same substitutions in both β-chains. This indicates that the αβ-heterodimer is a more appropriate model for studying the effects of myopathic mutations in the β-chain of Tpm than the ββ-homodimer which virtually does not exist in human skeletal muscles. 相似文献
8.
Dedova IV Nikolaeva OP Mikhailova VV dos Remedios CG Levitsky DI 《Biophysical chemistry》2004,110(1-2):119-128
Differential scanning calorimetry was used to examine the effects of cofilin on the thermal unfolding of actin. Stoichiometric binding increases the thermal stability of both G- and F-actin but at sub-saturating concentrations cofilin destabilizes F-actin. At actin:cofilin molar ratios of 1.5-6 the peaks corresponding to stabilized (66-67 degrees C) and destabilized (56-57 degrees C) F-actin are observed simultaneously in the same thermogram. Destabilizing effects of sub-saturating cofilin are highly cooperative and are observed at actin:cofilin molar ratios as low as 100:1. These effects are abolished by the addition of phalloidin or aluminum fluoride. Conversely, at saturating concentrations, cofilin prevents the stabilizing effects of phalloidin and aluminum fluoride on the F-actin thermal unfolding. These results suggest that cofilin stabilizes those actin subunits to which it directly binds, but destabilizes F-actin with a high cooperativity in neighboring cofilin-free regions. 相似文献
9.
Using a Synechocystis sp. PCC 6803 mutant strain that lacks photosystem (PS) I and that synthesizes chlorophyll (Chl) b, a pigment that is not naturally present in the wild-type cyanobacterium, the functional consequences of incorporation of this pigment into the PS II core complex were investigated. Despite substitution of up to 75% of the Chl a in the PS II core complex by Chl b, the modified PS II centers remained essentially functional and were able to oxidize water and reduce Q(A), even upon selective excitation of Chl b at 460 nm. Time-resolved fluorescence decay measurements upon Chl excitation showed a significant reduction in the amplitude of the 60-70 ps component of fluorescence decay in open Chl b-containing PS II centers. This may indicate slower energy transfer from the PS II core antenna to the reaction center pigments or slower energy trapping. Chl b and pheophytin b were present in isolated PS II reaction centers. Pheophytin b can be reversibly photoreduced, as evidenced from the absorption bleaching at approximately 440 and 650 nm upon illumination in the presence of dithionite. Upon excitation at 685 nm, transient absorption measurements using PS II particles showed some bleaching at 650 nm together with a major decrease in absorption around 678 nm. The 650 nm bleaching that developed within approximately 10 ps after the flash and then remained virtually unchanged for up to 1 ns was attributed to formation of reduced pheophytin b and oxidized Chl b in some PS II reaction centers. Chl b-containing PS II had a lower rate of charge recombination of Q(A)(-) with the donor side and a significantly decreased yield of delayed luminescence in the presence of DCMU. Taken together, the data suggest that Chl b and pheophytin b participate in electron-transfer reactions in PS II reaction centers of Chl b-containing mutant of Synechocystis without significant impairment of PS II function. 相似文献
10.
Temperature Sensitivity of Bacteriolysis Induced by β-Lactam Antibiotics in Amino Acid-Deprived Escherichia coli
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The temperature-sensitive penicillin tolerance response previously reported in amino acid-deprived Escherichia coli (W. Kusser and E. E. Ishiguro, J. Bacteriol. 169:2310–2312, 1987) was not due to the induction of the heat shock response resulting from a temperature upshift and was therefore unrelated to the findings of another report (J. K. Powell and K. D. Young, J. Bacteriol. 173:4021–4026, 1991) indicating a positive correlation between the expression of heat shock proteins and penicillin tolerance. The thermosensitive event occurred in the lysis induction stage. 相似文献