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1.
Patrick Lambert Jose Antonio Campoy Igor Pacheco Jehan-Baptiste Mauroux Cassia Da Silva Linge Diego Micheletti Daniele Bassi Laura Rossini Elisabeth Dirlewanger Thierry Pascal Michela Troggio Maria Jose Aranzana Andrea Patocchi Pere Arús 《Tree Genetics & Genomes》2016,12(6):121
One of the applications of genomics is to identify genetic markers linked to loci responsible for variation in phenotypic traits, which could be used in breeding programs to select individuals with favorable alleles, particularly at the seedling stage. With this aim, in the framework of the European project FruitBreedomics, we selected five main peach fruit characters and a resistance trait, controlled by major genes with Mendelian inheritance: fruit flesh color Y, fruit skin pubescence G, fruit shape S, sub-acid fruit D, stone adhesion-flesh texture F-M, and resistance to green peach aphid Rm2. They were all previously mapped in Prunus. We then selected three F1 and three F2 progenies segregating for these characters and developed genetic maps of the linkage groups including the major genes, using the single nucleotide polymorphism (SNP) genome-wide scans obtained with the International Peach SNP Consortium (IPSC) 9K SNP array v1. We identified SNPs co-segregating with the characters in all cases. Their positions were in agreement with the known positions of the major genes. The number of SNPs linked to each of these, as well as the size of the physical regions encompassing them, varied depending on the maps. As a result, the number of useful SNPs for marker-assisted selection varied accordingly. As a whole, this study establishes a sound basis for further development of MAS on these characters. Additionally, we also discussed some limitations that were observed regarding the SNP array efficiency. 相似文献
2.
E. Dirlewanger A. Moing C. Rothan L. Svanella V. Pronier A. Guye C. Plomion R. Monet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):18-31
The organoleptic quality of fleshy fruits is in a large part defined by their composition of soluble sugars and organic acids.
An F2 population issuing from a cross between two peach varieties, ‘Ferjalou Jalousia’, a non-acid peach, and ‘Fantasia’, an acid
nectarine, was analysed over 2 successive years for agronomic characters and for molecular-marker (isoenzymes, RFLPs, RAPDs,
IMAs and AFLPs) segregations. Blooming and maturity dates, as well as productivity, were noted for each tree. Four fruits
per tree were analysed at maturity for fresh weight, colour, pH, titratable acidity, soluble-solids content (SSC), acid (malic,
citric and quinic acids) and sugar (sucrose, glucose, fructose, sorbitol) contents. QTLs were detected for all fruit components
analysed, except for fruit colour. The QTLs for nearly all components were present on two linkage groups. For productivity,
fresh weight, pH, quinic acid, sucrose and sorbitol content, all the detected QTLs displayed the same effect as the parental
phenotypes. By contrast, for maturity date, titratable acidity, malic and citric acids and fructose, some QTLs displayed the
same effect as the parental phenotypes while others displayed the opposite effect. The fraction of the total variation in
each trait throughout the population explained by the QTLs was very high and reached more than 90% for some characters. For
most of the characters analysed, epistasis was observed between QTLs.
Received: 10 October 1997 / Accepted: 18 August 1998 相似文献
3.
Etienne C Moing A Dirlewanger E Raymond P Monet R Rothan C 《Physiologia plantarum》2002,114(2):259-270
As in many other fleshy fruits, the predominant organic acids in ripe peach ( Prunus persica (L.) Batsch) fruit are malic and citric acids. The accumulation of these metabolites in fruit flesh is regulated during fruit development. Six peach fruit-related genes implicated in organic acid metabolism (mitochondrial citrate synthase; cytosolic NAD-dependent malate dehydrogenase, and cytosolic NADP-dependent isocitrate dehydrogenase) and storage (vacuolar proton translocating pumps: one vacuolar H+ -ATPase, and two vacuolar H+ -pyrophosphatases) were cloned. Five of these peach genes were homologous to genes isolated from fruit in other fleshy fruit species. Phylogenetic and expression analyses suggested the existence of a particular vacuolar pyrophosphatase highly expressed in fruit. The sixth gene was the first cytosolic NAD-dependent malate dehydrogenase gene isolated from fruit. Gene expression was studied during the fruit development of two peach cultivars, a normal-acid (Fantasia) and a low-acid (Jalousia) cultivar. The overall expression patterns of the organic acid-related genes appeared strikingly similar for the two cultivars. The genes involved in organic acid metabolism showed a stronger expression in ripening fruit than during the earlier phases of development, but their expression patterns were not necessarily correlated with the changes in organic acid contents. The tonoplast proton pumps showed a biphasic expression pattern more consistent with the patterns of organic acid accumulation, and the tonoplast pyrophosphatases were more highly expressed in the fruit of the low-acid cultivar during the second rapid growth phase of the fruit. 相似文献
4.
Aranzana MJ Pineda A Cosson P Dirlewanger E Ascasibar J Cipriani G Ryder CD Testolin R Abbott A King GJ Iezzoni AF Arús P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(5):819-825
A set of 109 microsatellite primer pairs recently developed for peach and cherry have been studied in the almond x peach F(2) progeny previously used to construct a saturated Prunus map containing mainly restriction fragment length polymorphism markers. All but one gave amplification products, and 87 (80%) segregated in the progeny and detected 96 loci. The resulting Prunus map contains a total of 342 markers covering a total distance of 522 cM. The approximate position of nine additional simple sequence repeats (SSRs) was established by comparison with other almond and peach maps. SSRs were placed in all the eight linkage groups of this map, and their distribution was relatively even, providing a genome-wide coverage with an average density of 5.4 cM/SSR. Twenty-four single-locus SSRs, highly polymorphic in peach, and each falling within 24 evenly spaced approximately 25-cM regions covering the whole Prunus genome, are proposed as a 'genotyping set' useful as a reference for fingerprinting, pedigree and genetic analysis of this species. 相似文献
5.
Loïck Le Dantec Gaelle Cardinet Julio Bonet Mathieu Fouché Karima Boudehri Amparo Monfort Jean-Luc Poëssel Annick Moing Elisabeth Dirlewanger 《Tree Genetics & Genomes》2010,6(6):995-1012
The goal of the present study was to identify candidate genes (CGs) involved in fruit quality in peach that can be transferred to other Rosaceae species. Two cDNA libraries from fruit of the “Fantasia” peach cultivar, constructed at two stages of development, were used to generate a set of expressed sequence tag sequences. A total of 1,730 peach unigenes were obtained after clustering. Sequences and corresponding annotations were stored in a relational database and are available through a web interface. Fifty-nine CGs involved in fruit growth and development or fruit quality at maturity, focusing on sweetness, acidity, and phenolic compound content, were selected according to their annotation. Fifty-five primer pairs, designed from peach CG sequences and giving PCR products in peach, were tested in strawberry and 36 gave amplified products. Eight CGs were mapped in peach, 14 in strawberry, four in both species and confirmed the pattern of synteny already proposed using comparative mapping. In peach, the CGs are located in three linkage groups (3, 5, 7), and in strawberry they are distributed in all seven Fragaria linkage groups. Colocalization between some of these CGs and quantitative trait loci for fruit quality traits were identified and are awaiting confirmation in further analyses. 相似文献
6.
Antonio Cabrera Umesh R. Rosyara Paolo De Franceschi Audrey Sebolt Suneth S. Sooriyapathirana Elisabeth Dirlewanger Jose Quero-Garcia Mirko Schuster Amy F. Iezzoni Esther van der Knaap 《Tree Genetics & Genomes》2012,8(2):237-247
The Rosaceae Conserved Orthologous Set (RosCOS) provides a gene-based genome-wide set of markers that have been used in comparative
analyses of peach (Prunus persica), apple (Malus × domestica), and strawberry (Fragaria spp.). In order to extend the use of these RosCOS to sweet cherry (Prunus avium L.), we identified markers that are polymorphic in breeding germplasm. Ninety-five percent (595/627) of previously designed
RosCOS primer pairs amplified a product in six sweet cherry cultivars predicted to represent the range of genetic diversity
in breeding germplasm. A total of 45% (282/627) RosCOS were polymorphic among the six cultivars, and allele number ranged
from 2 to 6, with a genome-wide mean of 2.35. A subset of 92 genome-wide single nucleotide polymorphisms (SNPs) corresponding
to 76 RosCOS was analyzed in 36 founder accessions and progeny. The expected and observed heterozygosity suggested that 83%
of the RosCOS were in Hardy–Weinberg equilibrium, implying that most RosCOS behave as neutral markers. Principal coordinate
analysis (PCO) identified one wild accession and two Spanish landraces that clustered differently from the other accessions.
The relatively high number of unique alleles found in the three differentially clustered selections suggested that their use
as parents has potential to increase the genetic diversity in future US-bred cultivars. Of the 92 RosCOS SNPs, 81 SNPs that
represented 68 genome-wide RosCOS segregated in four mapping populations. These RosCOS were mapped in four F1 populations, thereby greatly improving the genetic linkage map of sweet cherry. 相似文献
7.
Gérald Seematter Mirjam Dirlewanger Valentine Rey Philippe Schneiter Luc Tappy 《Obesity (Silver Spring, Md.)》2002,10(1):49-55
Objective: To assess the short-term consequences of carbohydrate or fat overfeeding or of food restriction on the metabolic effects of mental stress in healthy lean women. Research Methods and Procedures: The effects of a sympathetic activation elicited by mental stress were evaluated in a group of healthy women after standardized isocaloric feeding (ISO) or after a 3-day overfeeding with 40% excess calories as either carbohydrate overfeeding (CHO OF) or fat overfeeding (FAT OF). Oxygen consumption rate (VO 2) was measured as an index of energy expenditure, and subcutaneous glycerol concentrations were monitored with microdialysis. The same measurements were performed in another group of healthy women after ISO and after a 3-day period of underfeeding with a protein sparing modified fast (UF). Results: In all conditions, mental stress significantly increased heart rate, blood pressure, plasma norepinephrine and epinephrine concentrations, and VO 2, and produced a nonsignificant increase in subcutaneous glycerol concentrations. CHO OF and FAT OF did not alter the effects of mental stress on VO 2 and subcutaneous glycerol concentrations. In contrast, UF increased basal VO 2 but significantly reduced its stimulation by mental stress. UF also enhanced the increase in subcutaneous glycerol concentrations during mental stress. Discussion: UF reduces the stimulation of energy expenditure and enhances lipolysis during sympathetic activation. These adaptations may be involved in mobilization of endogenous fat while limiting weight loss. In contrast, short-term overfeeding fails to alter the sympathetic control of energy expenditure and lipolysis. 相似文献
8.
D. Esmenjaud R. Voisin C. Van Ghelder N. Bosselut B. Lafargue M. Di Vito E. Dirlewanger J. L. Poëssel M. Kleinhentz 《Tree Genetics & Genomes》2009,5(2):279-289
Sources of resistance in Prunus spp. exhibit different spectra to the root-knot nematodes (RKN) Meloidogyne incognita, Meloidogyne javanica and Meloidogyne floridensis. In this Prunus genus, two dominant genes, Ma with a complete spectrum from the heterozygous Myrobalan plums P.2175 and P.2980 (section Euprunus; subgenus Prunophora) and RMia with a more restricted spectrum from the peaches Nemared and Shalil (subgenus Amygdalus), have been identified. This study characterizes the resistance spectra of interspecific crosses involving (1) previous Myrobalan
and peach sources, (2) two Alnem almonds (subgenus Amygdalus) resistant to M. javanica, and (3) the apricot A.3923, representing a species considered RKN-resistant (section Armeniaca; Prunophora). For both latter species, genetic data could be obtained through F1 crosses with genetically characterized Myrobalans that
conferred their rooting ability for clonal multiplication of the hybrids and permitted their simultaneous evaluation to the
three RKN. Crosses involving either Ma or RMia or both generated the expected resistance spectra. Nemared confirmed the species-specific resistance to M. incognita conferred by RMia. This rootstock, also previously considered resistant to M. javanica, was susceptible to the M. javanica isolate used, what illustrates an isolate-specific resistance to this species. Alnem accessions were shown homozygous resistant
to M. javanica. In the progeny P.2980 × A.3923, Ma markers allowed to distinguish resistant individuals carrying that gene from resistant individuals lacking it. Distribution
of non-Ma individuals in this cross suggested, in the apricot parent, (1) the absence of a major gene allelic to Ma and (2) the presence of a non RKN specific polygenic resistance. 相似文献
9.
The Ma gene for complete-spectrum resistance to Meloidogyne species in Prunus is a TNL with a huge repeated C-terminal post-LRR region 总被引:1,自引:0,他引:1
Claverie M Dirlewanger E Bosselut N Van Ghelder C Voisin R Kleinhentz M Lafargue B Abad P Rosso MN Chalhoub B Esmenjaud D 《Plant physiology》2011,156(2):779-792
Root-knot nematode (RKN) Meloidogyne species are major polyphagous pests of most crops worldwide, and cultivars with durable resistance are urgently needed because of nematicide bans. The Ma gene from the Myrobalan plum (Prunus cerasifera) confers complete-spectrum, heat-stable, and high-level resistance to RKN, which is remarkable in comparison with the Mi-1 gene from tomato (Solanum lycopersicum), the sole RKN resistance gene cloned. We report here the positional cloning and the functional validation of the Ma locus present at the heterozygous state in the P.2175 accession. High-resolution mapping totaling over 3,000 segregants reduced the Ma locus interval to a 32-kb cluster of three Toll/Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat (LRR) genes (TNL1-TNL3), including a pseudogene (TNL2) and a truncated gene (TNL3). The sole complete gene in this interval (TNL1) was validated as Ma, as it conferred the same complete-spectrum and high-level resistance (as in P.2175) using its genomic sequence and native promoter region in Agrobacterium rhizogenes-transformed hairy roots and composite plants. The full-length cDNA (2,048 amino acids) of Ma is the longest of all Resistance genes cloned to date. Its TNL structure is completed by a huge post-LRR (PL) sequence (1,088 amino acids) comprising five repeated carboxyl-terminal PL exons with two conserved motifs. The amino-terminal region (213 amino acids) of the LRR exon is conserved between alleles and contrasts with the high interallelic polymorphisms of its distal region (111 amino acids) and of PL domains. The Ma gene highlights the importance of these uncharacterized PL domains, which may be involved in pathogen recognition through the decoy hypothesis or in nuclear signaling. 相似文献
10.
RAPD and SCAR markers linked to the Ma1 root-knot nematode resistance gene in Myrobalan plum (Prunus cerasifera Ehr.) 总被引:1,自引:0,他引:1
A. C. Lecouls M. J. Rubio-Cabetas J. C. Minot R. Voisin A. Bonnet G. Salesses E. Dirlewanger D. Esmenjaud 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):328-335
The Myrobalan plum (Prunus cerasifera) is a self-incompatible species in which the clones P.2175, P.1079 and P.2980 are highly resistant to all root-knot nematodes
(RKN), Meloidogyne spp. Each clone bears a single major dominant gene, designated Ma1, Ma2 and Ma3 respectively, that controls a high and wide-spectrum resistance. Bulked segregant analysis (BSA) and random amplified polymorphic
DNA (RAPD) analysis were both performed to detect markers linked to the Ma1 gene using three segregating progenies from P.2175 (Ma1 ma1) crossed by three host parents (ma1 ma1). Four dominant coupling-phase markers were identified from a total of 660 10-base primers tested. The resulting linkage
map spans 14.7 cM and comprises three markers located on the same side of Ma1 and one marker located on the other side. The nearest markers (OPAL19720 and OPA161400) are located at 3.7 and 6.7 cM, respectively, on each side of the gene. Among the three markers that could be successfully
converted into sequence characterized amplified region (SCAR) markers, two of them (SCAL19690 and SCAN12620) were scored as dominant markers whereas the third (SCAO19770) failed to produce any polymorphism. SCAL19, and to a lesser extent SCAN12, can be used reliably in the marker-assisted selection
of Prunus rootstocks. These markers are adequate to identify the Ma1 RKN resistance gene in intraspecific segregating progenies and will be suitable for the creation of interspecific rootstocks
involving Myrobalan plum. Some of the RAPD and SCAR markers for Ma1 were also recovered in clones P.1079 and P.2980, but not in additional host clones, suggesting that Ma1, Ma2 and Ma3 are either allelic or at least closely linked.
Received: 22 September 1998 / Accepted: 19 December 1998 相似文献