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Prestin (SLC26a5) is the outer hair cell integral membrane motor protein that drives cochlear amplification, and has been described as an obligate tetramer. We studied in real time the delivery of YFP-prestin to the plasma membrane of cells from a tetracycline-inducible cell line. Following the release of temperature block to reinstate trans Golgi network delivery of the integral membrane protein, we measured nonlinear capacitance (NLC) and membrane fluorescence during voltage clamp. Prestin was delivered exponentially to the plasma membrane with a time constant of less than 10 minutes, with both electrical and fluorescence methods showing high temporal correlation. However, based on disparity between estimates of prestin density derived from either fluorescence or NLC, we conclude that sub-tetrameric forms of prestin contribute to our electrical and fluorescence measures. Thus, in agreement with previous observations we find that functional prestin is not an obligate tetramer. 相似文献
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Jing Chen Jialin Meng Yi Liu Zichen Bian Qingsong Niu Junyi Chen Jun Zhou Li Zhang Meng Zhang Chaozhao Liang 《Journal of cellular and molecular medicine》2022,26(21):5379
To identify prostate cancer (PCa) patients with a high risk of recurrence is critical before delivering adjuvant treatment. We developed a classifier based on the Enzalutamide treatment resistance‐related genes to assist the currently available staging system in predicting the recurrence‐free survival (RFS) prognosis of PCa patients. We overlapped the DEGs from two datasets to obtain a more convincing Enzalutamide‐resistance‐related‐gene (ERRG) cluster. The five‐ERRG‐based classifier obtained good predictive values in both the training and validation cohorts. The classifier precisely predicted RFS of patients in four cohorts, independent of patient age, pathological tumour stage, Gleason score and PSA levels. The classifier and the clinicopathological factors were combined to construct a nomogram, which had an increased predictive accuracy than that of each variable alone. Besides, we also compared the differences between high‐ and low‐risk subgroups and found their differences were enriched in cancer progression‐related pathways. The five‐ERRG‐based classifier is a practical and reliable predictor, which adds value to the existing staging system for predicting the RFS prognosis of PCa after radical prostatectomy, enabling physicians to make more informed treatment decisions concerning adjuvant therapy. 相似文献
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Hua Zhou Chunlei Yao Ao Bian Jun Qian Xiufen Zhao Yanting Zhao Weiwei Wang Changying Xing 《Molecular and cellular biochemistry》2014,391(1-2):21-25
Podocyte injury may contribute to the pathogenesis of diabetic nephropathy (DN), but the underlying mechanism of hyperglycemia induced podocyte damage is not fully understood. The Ras GTPase-activating-like protein IQGAP1 is associated to the slit diaphragm proteins and the actin cytoskeleton in podocyte. Here, we studied IQGAP1 expression alterations in human DN biopsies and extracellular signal-regulated kinase (ERK)-dependent pathways of IQGAP1 expression in podocyte under high glucose (HG) media. In vivo, analysis of renal biopsies from patients with DN revealed a significant reduction in IQGAP1 expression compared to controls. In vitro, IQGAP1 mRNA and protein expression were observed to decline under HG media at 48 h. But phosphorylation of ERK1/2 was activated under HG media at 24 h and 48 h. However, HG-induced downregulation of IQGAP1 protein was attenuated by specific ERK1/2 activation inhibitor PD98059. Taken together, these results highlight the importance of IQGAP1 in DN, and suggest that IQGAP1 expression in podocyte under HG media is modulated by the ERK1/2 pathway, which may lead to the future development of therapies targeting IQGAP1 dysfunction in podocytes in DN. 相似文献
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Fei Yang Ying Wang Shanshan Liu Chen He Xiaoqiu Tao Huimin Deng Gangling Tang Zhaoyang Bian Ziyan Fan 《Chirality》2020,32(5):505-514
We reported a new methodology for the stereoselective determination of metalaxyl enantiomers in tobacco and soil. The QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used for the extraction and clean-up of the tobacco and soil samples. Separation of the metalaxyl enantiomers was performed on an ACQUITY UPC2 Trefoil CEL1 chiral column coupled with supercritical fluid chromatography with tandem mass spectrometry (SFC-MS/MS), and the run time was only 5 minutes. Under the optimized conditions, the recoveries for the enantiomers were between 78.2% and 93.3% with intraday relative standard deviations (RSDs) ranging from 1.1% to 5.4%. The limit of detection (LOD) for the enantiomers in tobacco and soil varied from 0.005 to 0.007 mg/kg, and the limit of quantitation (LOQ) ranged from 0.017 to 0.020 mg/kg. In this method, only a small amount of methanol was consumed to obtain a rapid stereoselective separation. This proposed method showed good accuracy and precision and might be suitable for fast enantioselective determination of metalaxyl in food and environmental samples. The developed method was further validated by application to the analysis of authentic samples. 相似文献
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耐钙心肌细胞的分离和电生理特性观察 总被引:11,自引:1,他引:11
用快速、恒压的无钙和胶原酶Tyrode液相继灌流豚鼠心脏冠脉系统后,再经无钙液室温浸泡心脏和用改变的K-B液帮助分离细胞的恢复,可获得耐钙的游离心肌细胞。全细胞电流记录:静息电位为-72±9mV(n=12),并显示出快内向电流(INa),可被异搏定阻断的慢钙离子流和时间依赖性外向钾流(Ik);单通道记录分别显示了Na+Ca2+和K+通道的电压依赖性等特征。结果表明了用此法分离的细胞具有耐钙性和正常电生理特性。 相似文献
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Jing-Jing Wang Wen Sun Wei-Dong Jia Ming Bian Li-Jun Yu 《Journal of enzyme inhibition and medicinal chemistry》2022,37(1):2304
Oxadiazole is a five-membered heterocyclic compound containing two nitrogen atoms and one oxygen atom. The 1,3,4-oxadiazole and 1,2,4-oxadiazole have favourable physical, chemical, and pharmacokinetic properties, which significantly increase their pharmacological activity via hydrogen bond interactions with biomacromolecules. In recent years, oxadiazole has been demonstrated to be the biologically active unit in a number of compounds. Oxadiazole derivatives exhibit antibacterial, anti-inflammatory, anti-tuberculous, anti-fungal, anti-diabetic and anticancer activities. In this paper, we report a series of compounds containing oxadiazole rings that have been published in the last three years only (2020–2022) as there was no report or their activities described in any article in 2019, which will be useful to scientists in research fields of organic synthesis, medicinal chemistry, and pharmacology. 相似文献
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用抗生素或臭氧的方法诱导出四种细菌和L型后,用图像分析技术定量检测L型多形性,表面积、最大直径、周长、等效圆直径和形状因子,并作比较分析。四种细菌L型及L型中的巨形体在上述5项形态学参数指标上除形状因子外均较细菌型明显增大,而形状因子参数明显小于细菌型。细菌L型之间在形态学指标上的差异有显著性(p<0.05)。形态定量分析能准确客观地反映形态定性观察的结果以及细菌L型的形态变异程度,为细菌L型的形态定量分析提供依据。 相似文献