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1.
Chernonosov AA Kuznetsov NA Koval VV Pyshnyi DV Derkacheva NM Lukyanets EA Fedorova OS 《Nucleosides, nucleotides & nucleic acids》2004,23(6-7):983-987
Thermodynamics of interaction of phthalocyanine-oligonucleotide conjugates with single- and double-stranded DNA resulting in formation of duplexes and triplexes was measured by UV melting method. It was shown that a phthalocyanine moiety of conjugates stabilized the formation of duplexes and triplexes. 相似文献
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Pedro LC Pinheiro João CR Cardoso Ana S Gomes Juan Fuentes Deborah M Power Adelino VM Canário 《BMC evolutionary biology》2010,10(1):373
Background
Parathyroid hormone (PTH) and PTH-related peptide (PTHrP) belong to a family of endocrine factors that share a highly conserved N-terminal region (amino acids 1-34) and play key roles in calcium homeostasis, bone formation and skeletal development. Recently, PTH-like peptide (PTH-L) was identified in teleost fish raising questions about the evolution of these proteins. Although PTH and PTHrP have been intensively studied in mammals their function in other vertebrates is poorly documented. Amphibians and birds occupy unique phylogenetic positions, the former at the transition of aquatic to terrestrial life and the latter at the transition to homeothermy. Moreover, both organisms have characteristics indicative of a complex system in calcium regulation. This study investigated PTH family evolution in vertebrates with special emphasis on Xenopus and chicken. 相似文献3.
A. S. Chemeris A. V. Vakhrusheva N. I. Derkacheva O. S. Sokolova 《Moscow University Biological Sciences Bulletin》2018,73(1):1-6
The cytoskeleton is formed by a network of protein filaments, including microtubules, actin filaments and intermediate filaments. Filaments permeate the entire cytoplasm; they are involved in maintaining the cell shape, they organize and anchor the organelles, they control the transport of various molecules, cell division and provide signal transduction. To implement these diverse and complex functions, the components of the cytoskeleton must be very dynamic and mobile, be able to rebuilt quickly and interact with each other. This is due to the presence of a large number of actin-binding proteins—nucleators, activators, inactivators of polymerization and depolymerization of actin filaments. This review describes the regulation of actin dynamics by the Arp2/3 complex. In the cell, this complex is in an inactive state. Its activation occurs after it’s interaction with activators. Activators change the conformation and spatial arrangement of the domains of the Arp2/3 complex, providing its interaction with the monomeric and polymeric actin. Activators of the Arp2/3 complex have been known for a long time and include such proteins as WASp and WAVE. All activators possess a specific VCA domain, which is responsible for their binding to the Arp2/3 complex. The structure of the complex with bound activators has been studied using various physical-chemical methods. The inactivators of the complex only recently attracted specific attention of the investigators. At present, at least five different proteins are known to inactivate the Arp2/3 complex by binding to its various subunits. Examples of inactivators are coronin, Gmf and arpin. The structure of the Arp2/3 complex with inactivators was recently published and showed that despite their binding to different subunits of the complex, all inactivators transform the Arp2/3 complex into an “open” state, moving the actin-like Arp subunits apart from each other. Studies of the spatial organization of actin-binding proteins are necessary for understanding the patterns of interaction between them while providing the vital activity of the cell. These data can later be used in the search for new ligands to prevent metastasis of tumor cells. 相似文献
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Background
Although direct infiltration of papillary carcinoma of thyroid to larynx, trachea and esophagus is well recognized, lymphatic and vascular metastases to larynx and hypopharynx have rarely been reported. 相似文献7.
Koval VV Chernonosov AA Abramova TV Ivanova TM Fedorova OS Derkacheva VM Lukyanets EA 《Nucleosides, nucleotides & nucleic acids》2001,20(4-7):1259-1262
The synthesis of new metallophthalocyanine-oligonucleotide conjugates is reported. These conjugates can cause sequence-specific photosensitized or catalytic oxidation of DNA by molecular oxygen. 相似文献
8.
Application of fourier transform infrared spectroscopy for the study of lignins of herbaceous plants
The results of a study of dioxane lignins isolated from several herbaceous plants using FTIR spectroscopy are presented in the work. The presence of a strong relationship between parameters of absorption bands and the content of various structural units and functional groups of lignins has been demonstrated. 相似文献
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Maria Derkacheva Yvonne Steinbach Thomas Wildhaber Iva Mozgová Walid Mahrez Paolo Nanni Wilhelm Gruissem Lars Hennig 《The EMBO journal》2013,32(14):2073-2085
Polycomb group (PcG) proteins form essential epigenetic memory systems for controlling gene expression during development in plants and animals. However, the mechanism of plant PcG protein functions remains poorly understood. Here, we probed the composition and function of plant Polycomb repressive complex 2 (PRC2). This work established the fact that all known plant PRC2 complexes contain MSI1, a homologue of Drosophila p55. While p55 is not essential for the in vitro enzymatic activity of PRC2, plant MSI1 was required for the functions of the EMBRYONIC FLOWER and the VERNALIZATION PRC2 complexes including trimethylation of histone H3 Lys27 (H3K27) at the target chromatin, as well as gene repression and establishment of competence to flower. We found that MSI1 serves to link PRC2 to LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a protein that binds H3K27me3 in vitro and in vivo and is required for a functional plant PcG system. The LHP1–MSI1 interaction forms a positive feedback loop to recruit PRC2 to chromatin that carries H3K27me3. Consequently, this can provide a mechanism for the faithful inheritance of local epigenetic information through replication. 相似文献
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Cyanobacterium Anacystis nidulans R2, Synechocystis sp. PCC 6803 (wild-type strain and mutants Delta2 and Delta3 lacking PSII and PSI, respectively), and Synechocystis sp. BO 9201 synthesize the pigment--protein complex CP36 (CPIV-4, CP43') under iron deficiency in the medium. Accumulation of CP36 is accompanied by structural reorganizations in the photosynthetic membranes. Integrating mean times of excitation relaxation (quenching) are 2.2 nsec (CP36), 1 nsec (PSI), and 420 psec (PSII in Fm state). The energy migration between CP36 and the photosystems can be described by a model of a one-layer ring of CP36 around core-complexes. The excitation from CP36 to PSI is transferred within <10 psec. The energy transfer from CP36 to PSII occurs during 170 psec. Cells with low content of CP36 probably contain only a latent fraction of unbound to phycobilisomes PSII which is the analog of PSIIbeta of higher plants. In PSI there are four binding sites for CP36 monomers per RC. PSII can bind up to 32 molecules of CP36 per RC. Cells with a large amount of CP36 contain monomer form of PSII core-complex which can bind eight tetramers of CP36 (8 binding sites). In conditions of iron deficiency only one monomer of a dimer PSII core-complex is destroyed and released chlorophyll is accumulated in CP36. Accumulation of CP36 in A. nidulans cells can be accompanied by membrane stacking which is similar to the stacking in chlorophyll b-containing organisms. The stacking can occur in the region of localization of PSII latent fraction bound to CP36. The membrane stacking shields PSII stromal surfaces from the aqueous phase for activation of electron transfer on the acceptor side of PSII. 相似文献