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1.
The dynamics of Antarctic coastal marine bacterioplankton has been studied over a 2-year period. Two field stations were sampled
between one and three times a week in 1989 and 1991 in the “Terre Adélie” area. The survey included physicochemical (temperature
and particulate organic matter) and bacteriological (total and heterotrophic counts, cell volume and frequency of dividing
cells estimation) measurements. The results suggest that a strong interannual variability affects the total bacterial abundance,
the mean cell volume, the percentage of free living cells and, to a lesser extent. the culturable saprophytic bacterial communities.
The observed variability could be partly explained by a large deficit of solar irradiance during the 2nd year of study that
may have affected sea ice and seawater primary production. 相似文献
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Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking water biostability by monitoring in situ the evolution of the attenuated total reflectance-Fourier transform infrared (ATR-FTIR) fingerprint of a nascent reference biofilm exposed to water being tested. For this purpose, the responses of nascent Pseudomonas fluorescens biofilms to variations in the dissolved organic carbon (DOC) level in tap water were monitored in situ and in real time by ATR-FTIR spectroscopy. Nascent P. fluorescens biofilms consisting of a monolayer of bacteria were formed on the germanium crystal of an ATR flowthrough cell by pumping bacterial suspensions in Luria-Bertani (LB) medium through the cell. Then they were exposed to a continuous flow of dechlorinated sterile tap water supplemented with appropriate amounts of sterile LB medium to obtain DOC concentrations ranging from 1.5 to 11.8 mg/liter. The time evolution of infrared bands related to proteins, polysaccharides, and nucleic acids clearly showed that changes in the DOC concentration resulted in changes in the nascent biofilm ATR-FTIR fingerprint within 2 h after exposure of the biofilm to the water being tested. The initial bacterial attachment, biofilm detachment, and regrowth kinetics determined from changes in the areas of bands associated with proteins and polysaccharides were directly dependent on the DOC level. Furthermore, they were consistent with bacterial adhesion or growth kinetic models and extracellular polymeric substance overproduction or starvation-dependent detachment mechanisms. 相似文献
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Biodegradation by naturally occurring populations of microorganisms is a major mechanism for the removal of oil hydrocarbons from the environment. Therefore, follow-up of bacterial populations and chemical indices of biodegradation are important components of contaminated site assessment studies. Over a 4-year period following an accidental diesel contamination of the sub-Antarctic Crozet Archipelago (51°51′E–46°25′S), a field study was carried out in the contaminated area that is located in a transition zone between an arid fell-field (upstream) and a wet vegetated area (downstream). This study included a monitoring of heterotrophic and hydrocarbon-degrading bacterial abundance and chemical analysis of the remaining hydrocarbons. Significant higher number of heterotrophic and hydrocarbon-degrading bacterial counts revealed a rapid acclimation of sub-Antarctic microbial soil communities to the diesel fuel contamination. A chemical survey conducted during the last 2 years (2002 and 2003) showed that the total extractable hydrocarbons (TPH) content in arid fell field was reduced to ≤50% of their value while it was reduced only to ≤65% in vegetated soil. In addition, the decrease of TPH was always higher in the presence of fertilizer in the arid contaminated area, while fertilizer addition was almost inefficient in the wet contaminated one. All these results demonstrate a serious influence of the soil properties on the degradation rate. However, all chemical indices showed a significant reduction of alkanes and light aromatics in both contaminated area confirming a regular oil degradation process. 相似文献
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Biological treatment has become increasingly popular as a remediation method for soils and groundwater contaminated with petroleum hydrocarbon, chlorinated solvents, and pesticides. Bioremediation has been considered for application in cold regions such as Arctic and sub-Arctic climates and Antarctica. Studies to date suggest that indigenous microbes suitable for bioremediation exist in soils in these regions. This paper reports on two case studies at the sub-Antarctic Kerguelen Island in which indigenous bacteria were found that were capable of mineralizing petroleum hydrocarbons in soil contaminated with crude oil and diesel fuel. All results demonstrate a serious influence of the soil properties on the biostimulation efficiency. Both temperature elevation and fertilizer addition have a more significant impact on the microbial assemblages in the mineral soil than in the organic one. Analysis of the hydrocarbons remaining at the end of the experiments confirmed the bacterial observations. Optimum temperature seems to be around 10 degrees C in organic soil, whereas it was higher in mineral soil. The benefit of adding nutrients was much stronger in mineral than in the organic soil. Overall, this study suggests that biostimulation treatments were driven by soil properties and that ex situ bioremediation for treatment of cold contaminated soils will allow greater control over soil temperature, a limiting factor in cold climates. 相似文献
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In Situ Monitoring of the Nascent Pseudomonas fluorescens Biofilm Response to Variations in the Dissolved Organic Carbon Level in Low-Nutrient Water by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy
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Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking water biostability by monitoring in situ the evolution of the attenuated total reflectance-Fourier transform infrared (ATR-FTIR) fingerprint of a nascent reference biofilm exposed to water being tested. For this purpose, the responses of nascent Pseudomonas fluorescens biofilms to variations in the dissolved organic carbon (DOC) level in tap water were monitored in situ and in real time by ATR-FTIR spectroscopy. Nascent P. fluorescens biofilms consisting of a monolayer of bacteria were formed on the germanium crystal of an ATR flowthrough cell by pumping bacterial suspensions in Luria-Bertani (LB) medium through the cell. Then they were exposed to a continuous flow of dechlorinated sterile tap water supplemented with appropriate amounts of sterile LB medium to obtain DOC concentrations ranging from 1.5 to 11.8 mg/liter. The time evolution of infrared bands related to proteins, polysaccharides, and nucleic acids clearly showed that changes in the DOC concentration resulted in changes in the nascent biofilm ATR-FTIR fingerprint within 2 h after exposure of the biofilm to the water being tested. The initial bacterial attachment, biofilm detachment, and regrowth kinetics determined from changes in the areas of bands associated with proteins and polysaccharides were directly dependent on the DOC level. Furthermore, they were consistent with bacterial adhesion or growth kinetic models and extracellular polymeric substance overproduction or starvation-dependent detachment mechanisms. 相似文献
9.
Daniel Delille Emilien Pelletier Arturo Rodriguez-Blanco Jean-Francois Ghiglione 《Polar Biology》2009,32(10):1521-1528
In an attempt to evaluate the potential of petroleum bioremediation at high latitudes environments, microcosm studies using
Antarctic coastal seawater contaminated with diesel or crude oil were conducted in Kerguelen Archipelago (49°22′S, 70°12′E).
Microcosms were incubated at three different temperatures (4, 10 and 20°C). During experiments, changes observed in microbial
assemblages (total direct count, heterotrophic cultivable microorganisms and hydrocarbon-degrading microorganisms) were generally
similar for all incubation temperatures, but chemical data showed only some slight changes in biodegradation indices [Σ(C12–C20)/Σ(C21–C32)
and C17/pristane]. The complete data set provided strong evidence of the presence of indigenous hydrocarbon-degrading bacteria
in Antarctic seawater and their high potential for hydrocarbon bioremediation. The rate of oil degradation could be increased
by the addition of a commercial fertilizer, but water temperature had little effects on biodegradation efficiency which is
in conflict with the typical temperature-related assumption predicting 50% rate reduction when temperature is reduced by 10°C.
Global warming of Antarctic seawater should not increase significantly the rate of oil biodegradation in these remote regions. 相似文献
10.
Chuan-Ming Xie Xiao-Yu Liu Kathy WY Sham Josie MY Lai Christopher HK Cheng 《Autophagy》2014,10(9):1495-1508
EEF2K (eukaryotic elongation factor-2 kinase), also known as Ca2+/calmodulin-dependent protein kinase III, functions in downregulating peptide chain elongation through inactivation of EEF2 (eukaryotic translation elongation factor 2). Currently, there is a limited amount of information on the promotion of autophagic survival by EEF2K in breast and glioblastoma cell lines. However, the precise role of EEF2K in carcinogenesis as well as the underlying mechanism involved is still poorly understood. In this study, contrary to the reported autophagy-promoting activity of EEF2K in certain cancer cells, EEF2K is shown to negatively regulate autophagy in human colon cancer cells as indicated by the increase of LC3-II levels, the accumulation of LC3 dots per cell, and the promotion of autophagic flux in EEF2K knockdown cells. EEF2K negatively regulates cell viability, clonogenicity, cell proliferation, and cell size in colon cancer cells. Autophagy induced by EEF2K silencing promotes cell survival and does not potentiate the anticancer efficacy of the AKT inhibitor MK-2206. In addition, autophagy induced by silencing of EEF2K is attributed to induction of protein synthesis and activation of the AMPK-ULK1 pathway, independent of the suppression of MTOR activity and ROS generation. Knockdown of AMPK or ULK1 significantly abrogates EEF2K silencing-induced increase of LC3-II levels, accumulation of LC3 dots per cell as well as cell proliferation in colon cancer cells. In conclusion, silencing of EEF2K promotes autophagic survival via activation of the AMPK-ULK1 pathway in colon cancer cells. This finding suggests that upregulation of EEF2K activity may constitute a novel approach for the treatment of human colon cancer. 相似文献