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排序方式: 共有100条查询结果,搜索用时 15 毫秒
1.
A N Siniakov O I Serpinski? N K Daniliuk V E Chizhikov S Kh Degtiarev 《Bioorganicheskaia khimiia》1989,15(5):638-647
For preparing a DNA fragment with unique protruding ends, plasmid vectors pMB123 and pMB124 were constructed by inserting a synthetic polylinker into plasmid pUR222 at the EcoRI-PstI sites. The polylinker contains two FokI and HgaI sites at its ends in opposite orientation flanking a combination of SalGI, AccI, HindII, HindIII (the latter site is absent from pMB124) and BamHI sites. DNA fragment cloned at the SalGI and BamHI sites can be regenerated by either FokI or HgaI treatment, the SalGI and BamHI sites being deleted from the cloned sequence. Fragments coding for parts of human interleukin-2 were cloned in these vectors. 相似文献
2.
P A Belavin Iu A Gorbunov E G Malygin N I Rechkunova S Kh Degtiarev 《Bioorganicheskaia khimiia》1989,15(3):417-418
The specificity of DNA methylase M. FokI towards oligonucleotides containing sequence 5'...GGATG.../3'...CCTAC... was investigated, and N6-methyladenine in the GGATG chain was shown to be the only product of the modification. 相似文献
3.
I L Degtiarev E I Shumakova S M Vorob'ev D A Kostiuk S N Kochetkov 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1989,(9):20-22
The monoclonal antibodies to DNA-dependent RNA-polymerase of bacteriophage T7 have been obtained. Twenty of the obtained 500 clones have inhibited the enzyme activity. Three specificity groups were identified for seven of the clones supporting their affinity for different antigenic determinants. 相似文献
4.
5.
Fushitani K; Higashiyama K; Moriyama EN; Imai K; Hosokawa K 《Molecular biology and evolution》1996,13(7):1039-1043
To elucidate phylogenetic relationships among amniotes and the evolution of
alpha globins, hemoglobins were analyzed from the Komodo dragon (Komodo
monitor lizard) Varanus komodoensis, the world's largest extant lizard,
inhabiting Komodo Islands, Indonesia. Four unique globin chains (alpha A,
alpha D, beta B, and beta C) were isolated in an equal molar ratio by high
performance liquid chromatography from the hemolysate. The amino acid
sequences of two alpha chains were determined. The alpha D chain has a
glutamine at E7 as does an alpha chain of a snake, Liophis miliaris, but
the alpha A chain has a histidine at E7 like the majority of hemoglobins.
Phylogenetic analyses of 19 globins including two alpha chains of Komodo
dragon and ones from representative amniotes showed the following results:
(1) The a chains of squamates (snakes and lizards), which have a glutamine
at E7, are clustered with the embryonic alpha globin family, which
typically includes the alpha D chain from birds; (2) birds form a sister
group with other reptiles but not with mammals; (3) the genes for embryonic
and adult types of alpha globins were possibly produced by duplication of
the ancestral alpha gene before ancestral amniotes diverged, indicating
that each of the present amniotes might carry descendants of the two types
of alpha globin genes; (4) squamates first split off from the ancestor of
other reptiles and birds.
相似文献
6.
7.
Okhapkina SS Netesova NA Golikova LN Seregina EV Sosnovtsev SV Abdurashitov MA Degtiarev SKh 《Molekuliarnaia biologiia》2002,36(3):432-437
A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'. A substantial difference was that SfeI RMS operon had an additional 198-bp fragment and a larger gene for the putative control protein. No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon. 相似文献
8.
本文用脑室灌注和Fura2测定细胞内游离钙技术观察了地塞米松(dexamethasone,DEX)对家兔乙二醇双(2氨基乙醚)四乙酸性发热效应和下丘脑细胞内游离钙浓度([Ca2+]i)的影响,借此深入探讨地塞米松解热作用的中枢机制。结果发现:脑室灌注乙二醇双(2氨基乙醚)四乙酸(06nmol)引起家兔结肠温度明显升高,静脉注射地塞米松(5mg/kg)显著抑制家兔乙二醇双(2氨基乙醚)四乙酸性发热,地塞米松(60~120μmol/L)并不影响下丘脑细胞内[Ca2+]i,而事先脑室灌注抑制基因转录的放线菌素D(3nmol)则完全取消了地塞米松对乙二醇双(2氨基乙醚)四乙酸性发热的解热作用。这些结果提示:地塞米松显著抑制家兔乙二醇双(2氨基乙醚)四乙酸性发热,其机制与地塞米松激活脑内某些基因的表达有关,而与下丘脑神经细胞跨膜钙离子流无关。 相似文献
9.
Matrix metalloproteinases (MMPs) degrade components of the extracellular matrix of the disc, but the presence of MMP-19 has not been explored. In other tissues, MMP-19 is known to act in proteolysis of the insulin-like growth factor (IGF) binding protein-3, thereby exposing this protein to make it available to influence cell behavior. MMP-19 also has been shown to inhibit capillary-like formation and thus play a role in the avascular nature of the disc. Using immunohistochemistry, normal discs from six subjects aged newborn through 10 years and 20 disc specimens from control donors or surgical patients aged 15-76 (mean age 40.2 years) were examined for immunolocalization of MMP-19; six Thompson grade I discs, five Thompson grade II, eight Thompson grade III, five Thompson grade IV, and one Thompson grade V discs were analyzed. The results indicate that in discs from young subjects, MMP-19 was uniformly localized in the outer annulus. In discs from adult donors and surgical patients, outer and inner annulus cells only occasionally showed MMP-19 localization. The greatest expression of MMP-19 was observed in young discs, and little expression was seen in older or degenerating discs. Because MMP-19 has been shown to regulate IGF-mediated proliferation in other tissues, its decline in the aging/degenerating disc may contribute to the age-related decrease in disc cell numbers. 相似文献
10.
竹叶眼子菜(Potamogeton malaianus)、微齿眼子菜(Potamogeton maackianus)、苦草(Vallisneria spiralis)、穗花狐尾藻(Myriophyllum spicatum)、黑藻(Hydrilla verticillata)是长江中下游湖泊主要的沉水植物。在栽培条件下,它们的无性繁殖速率(单位时间内新增个体数)大小顺序为黑藻>微齿眼子菜>竹叶眼子菜>苦草>穗花狐尾藻。同时采用抛掷实验的方法观察研究了这5种沉水植物及其无性繁殖体的存活和生根情况;完整植株存活率为黑藻>穗花狐尾藻>微齿眼子菜>竹叶眼子菜>苦草,无性繁殖体部分存活率为黑藻>苦草>穗花狐尾藻>微齿眼子菜>竹叶眼子菜。生根能力和其存活时间长短相关,而且生根能力与存活率大小基本一致。在实验中,只有穗花狐尾藻的断枝存活率和生根能力存在差异,故无性繁殖体生根能力为黑藻>苦草>微齿眼子菜>竹叶眼子菜>穗花狐尾藻。 相似文献