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排序方式: 共有163条查询结果,搜索用时 31 毫秒
1.
Lipoprotein lipase gene expression in THP-1 cells 总被引:5,自引:0,他引:5
2.
K Fukuchi K Kamino S S Deeb A C Smith T Dang G M Martin 《Biochemical and biophysical research communications》1992,182(1):165-173
The recent discovery that point mutations in the beta/A4 amyloid precursor protein may be the cause of certain forms of familial Alzheimer's disease provides strong support for the view that a thorough understanding of the metabolism of this protein may elucidate the pathogenesis of most forms of the disease and thus serve as a basis for rational prevention and therapy. Here we show that overexpression of a portion of the amyloid precursor protein molecule produces at least four distinct fragments of the COOH-terminus of amyloid precursor protein, suggesting altered proteolysis of amyloid precursor protein, and that such overexpression is associated with cytotoxicity. The degree of toxicity in the P19 cell culture model (differentiating mouse embryonal carcinoma cells) is shown to be related to the two larger novel COOH-terminal protein fragments (16 and 14 kilodalton), as well as to levels of expression of these two fragments. The toxicity is manifested in several differentiated cell lineages, including neuronal cells. 相似文献
3.
G M Deeb C M Grum M J Lynch T P Guynn K P Gallagher A G Ljungman S F Bolling M L Morganroth 《Journal of applied physiology》1990,68(1):374-381
Ischemia-reperfusion lung injury limits lung transplantation. Neutrophil activation and/or xanthine oxidase-mediated purine degradation may cause toxic oxygen metabolite production and lung injury. We investigated whether circulating blood elements are involved in the pathogenesis of ischemia-reperfusion lung injury. Isolated rat lungs were perfused with physiological salt solution (PSS) stabilized with Ficoll until circulating blood elements were not detected in the lung effluent. Lungs were then rendered ischemic by stopping ventilation and perfusion for 45 min at room temperature. Lung injury occurred and was quantitated by the accumulation of 125I-bovine serum albumin into lung parenchyma and alveolar lavage fluid during reperfusion. Lung injury occurred, in the absence of circulating blood elements, when ischemic lungs were reperfused with PSS-Ficoll solution alone. Reperfusion with whole blood or PSS-Ficoll supplemented with human or rat neutrophils did not increase lung injury. Furthermore, during lung ischemia, the presence of neutrophils did not enhance injury. Experiments using PSS-albumin perfusate and quantitating lung injury by permeability-surface area product yielded similar results. Microvascular pressures were not different and could not account for the results. Toxic O2 metabolites were involved in the injury because addition of erythrocytes or catalase to the perfusate attenuated the injury. Thus reperfusion after lung ischemia causes injury that is dependent on a nonneutrophil source of toxic O2 metabolites. 相似文献
4.
FRIEDRICH BEERMANN ERIKA SCHMID RUTH GANSS GÜNTHER SCHÜTZ SIEGFRIED RUPPERT 《Pigment cell & melanoma research》1992,5(5):295-299
Tyrosinase is the key enzyme in melanin synthesis, and is expressed in the pigment epithelium of the retina, a cell layer derived from the optic cup; and in neural crest-derived melanocytes of skin, hair follicle, choroid, and iris. The tyrosinase gene has been cloned and shown to map to the well-characterized c-locus (albino locus) of the mouse. Subsequent studies demonstrated that a functional tyrosinase minigene was able to rescue the albino phenotype in transgenic mice. The transgene was expressed in a cell type-specific manner in skin and eye. During development of the mouse, the tyrosinase gene is expressed in the pigment epithelium of the retina as early as day 10.5 of gestation. In the hair follicle, tyrosinase gene expression is detected from day 16.5 onwards. This cell-type–specific expression is largely reproduced in transgenic mice. Our results suggest that sequences in the immediate vicinity of the mouse tyrosinase gene are sufficient to provide cell type-specificity and developmental regulation in melanocytes and the pigment epithelium. 相似文献
5.
Coexistence of abnormalities of hepatic lipase and lipoprotein lipase in a large family. 总被引:5,自引:0,他引:5
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J H Auwerx S P Babirak J E Hokanson G Stahnke H Will S S Deeb J D Brunzell 《American journal of human genetics》1990,46(3):470-477
A large family is reported with familial hepatic triglyceride lipase (HTGL) deficiency and with the coexistence of reduced lipoprotein lipase (LPL) similar to the heterozygote state of LPL deficiency. The proband was initially detected because of hypertriglyceridemia and chylomicronemia. He was later demonstrated to have beta-VLDL despite an apo E3/E3 phenotype and the lack of stigmata of type III hyperlipoproteinemia. The proband had no HTGL activity in postheparin plasma. Two of his half-sisters had very low HTGL activity (39 and 31 nmol free fatty acids/min/ml; normal adult female greater than 44). His son and daughters had decreased HTGL activity (normal male and preadolescent female greater than 102), which would be expected in obligate heterozygotes for HTGL deficiency. Low HTGL activity was associated with LDL particles which were larger and more buoyant. Several family members, including the proband, had reduced LPL activity and mass less than that circumscribed by the 95% confidence-interval ellipse for normal subjects and had hyperlipidemia similar to that described in heterozygote relatives of patients with LPL deficiency. All the sibs with hyperlipidemia had a reduced LPL activity and mass, while subjects with isolated reduced HTGL (with normal LPL activity) had normal lipid phenotypes. Analysis of genomic DNA from these subjects by restriction-enzyme digestion revealed no major abnormalities in the structure of either the HTGL or the LPL gene. Compound heterozygotes for HTGL and LPL deficiency show lipoprotein physiological characteristics typical for HTGL deficiency, while their variable lipid phenotype is typical for LPL deficiency. 相似文献
6.
Deeb TZ Carland JE Cooper MA Livesey MR Lambert JJ Peters JA Hales TG 《The Journal of biological chemistry》2007,282(9):6172-6182
Structural models suggest that Arg(436) lies within five cytoplasmic portals of the 5-HT(3A) receptor. We tested both the accessibility of residue 436 and the influence of its charge on single channel conductance (gamma) by substituting Arg(436) with Cys and examining the effect of methanethiosulfonate (MTS) reagents on gamma. Inclusion of positively charged 2-aminoethyl-MTS (MTSEA) within the electrode solution reduced gamma of 5-HT(3A)(R436C) receptors in outside-out patches from 7.8 +/- 0.5 to 5.0 +/- 0.5 picosiemens (pS). To increase gamma, we substituted Arg(436) by Cys in the 5-HT(3A)(R432Q,R440A) mutant, yielding the 5-HT(3A)(QCA) construct with a gamma of 17.7 +/- 0.4 pS. Modification of 5-HT(3A)(QCA) receptors by MTSEA or 2-(trimethylammonium) ethyl-MTS reduced gamma to 8.7 +/- 0.5 and 6.7 +/- 0.4 pS, respectively, both significantly below that of channels exposed to nonpolar propyl-MTS. Extracellular MTSEA, but not 2-(trimethylammonium) ethyl-MTS, crossed the membrane and in so doing slowly (tau = 94 s) reduced gamma. MTSEA more rapidly (tau = 15 s) reduced the gamma of 5-HT(3A)(QCA) receptors in inside-out patches, an effect reversed by the reducing agent dithiothreitol. Cys(436) modification by negatively charged 2-carboxyethyl-MTS and 2-sulfonatoethyl-MTS increasedgamma to 23 +/- 1.0 and 26 +/- 0.7 pS, respectively. MTS reagents did not affect gamma values for 5-HT(3A)(QDA) constructs with Cys substituted for Lys(431) predicted to be outside the entrance to the portals. Collectively, the data demonstrate that the dynamic modification of the charge of a cytoplasmic residue regulates gamma, consistent with the existence of cytoplasmic portals that impose a rate-limiting barrier to ion conduction in Cys loop receptors. 相似文献
7.
Structural and functional consequences of missense mutations in exon 5 of the lipoprotein lipase gene 总被引:2,自引:0,他引:2
Peterson J Ayyobi AF Ma Y Henderson H Reina M Deeb SS Santamarina-Fojo S Hayden MR Brunzell JD 《Journal of lipid research》2002,43(3):398-406
Missense mutations in exon 5 of the LPL gene are the most common reported cause of LPL deficiency. Exon 5 is also the region with the strongest homology to pancreatic and hepatic lipase, and is conserved in LPL from different species. Mutant LPL proteins from post-heparin plasma from patients homozygous for missense mutations at amino acid positions 176, 188, 194, 205, and 207, and from COS cells transiently transfected with the corresponding cDNAs were quantified and characterized, in an attempt to determine which aspect of enzyme function was affected by each specific mutation. All but one of the mutant proteins were present, mainly as partially denatured LPL monomer, rendering further detailed assessment of their catalytic activity, affinity to heparin, and binding to lipoprotein particles difficult. However, the fresh unstable Gly(188)-->Glu LPL and the stable Ile(194)-->Thr LPL, although in native conformation, did not express lipase activity. It is proposed that many of the exon 5 mutant proteins are unable to achieve or maintain native dimer conformation, and that the Ile(194)-->Thr substitution interferes with access of lipid substrate to the catalytic pocket. These results stress the importance of conformational evaluation of mutant LPL. Absence of catalytic activity does not necessarily imply that the substituted amino acid plays a specific direct role in catalysis. 相似文献
8.
Qiuying Chen Ruba S. Deeb Yuliang Ma Michelle R. Staudt Ronald G. Crystal Steven S. Gross 《PloS one》2015,10(12)
COPD (chronic obstructive pulmonary disease) is defined by a fixed expiratory airflow obstruction associated with disordered airways and alveolar destruction. COPD is caused by cigarette smoking and is the third greatest cause of mortality in the US. Forced expiratory volume in 1 second (FEV1) is the only validated clinical marker of COPD, but it correlates poorly with clinical features and is not sensitive enough to predict the early onset of disease. Using LC/MS global untargeted metabolite profiling of serum samples from a well-defined cohort of healthy smokers (n = 37), COPD smokers (n = 41) and non-smokers (n = 37), we sought to discover serum metabolic markers with known and/or unknown molecular identities that are associated with early-onset COPD. A total of 1,181 distinct molecular ions were detected in 95% of sera from all study subjects and 23 were found to be differentially-expressed in COPD-smokers vs. healthy-smokers. These 23 putative biomarkers were differentially-correlated with lung function parameters and used to generate a COPD prediction model possessing 87.8% sensitivity and 86.5% specificity. In an independent validation set, this model correctly predicted COPD in 8/10 individuals. These serum biomarkers included myoinositol, glycerophopshoinositol, fumarate, cysteinesulfonic acid, a modified version of fibrinogen peptide B (mFBP), and three doubly-charged peptides with undefined sequence that significantly and positively correlate with mFBP levels. Together, elevated levels of serum mFBP and additional disease-associated biomarkers point to a role for chronic inflammation, thrombosis, and oxidative stress in remodeling of the COPD airways. Serum metabolite biomarkers offer a promising and accessible window for recognition of early-stage COPD. 相似文献
9.
10.
Deeb Taher 《Inorganica chimica acta》2006,359(6):1899-1906
Mononuclear and homobimetallic palladium complexes of structural type [trans-(Me(O)CS-4-C6H4)(Ph3P)2Pd(N∩N)]OTf (8a, N∩NC4H4N2; 8b, N∩NC5H4N-4-CN) and {[trans-(Me(O)CS-4-C6H4)(Ph3P)2Pd]2N∩N}(OTf)2 (9a, N∩N = 4,4′-bipyridine (=bipy); 9b, N∩N = C6H4-1,4-(CN)2; 9c, N∩N = (C6H4-4-CN)2) are accessible by the reaction of trans-(Ph3P)2Pd(C6H4-4-SC(O)Me)(OTf) (6) with 1 or 0.5 equivalents of the Lewis-bases N∩N (7a, N∩N = C4H4N2; 7b, N∩N = C5H4N-4-CN; 7c, N∩N = bipy; 7d, N∩N = C6H4-1,4-(CN)2; 7e, N∩N = (C6H4-4-CN)2) in high yield. Complex 6 can be prepared in a two-step synthesis procedure. Oxidative addition of I-1-C6H4-4-SC(O)Me (2) to Pd(PPh3)4 (3) gives trans-(Ph3P)2Pd(C6H4-4-SC(O)Me)(I) (4), which further reacts with [AgOTf] (5) to afford 6.The formation of 8 and 9 strongly depends on the size of the Lewis-bases N∩N. It is obvious that the co-ordination of the second N-ligated site of 8a or 8b to a further bulky[(PPh3)2Pd(C6H4-4-SC(O)Me)]+ unit is not possible. In contrast, more extended N∩N species such as 7c-7e will result in the formation of linear structured homobimetallic 9a-9c.The solid-state structures of 4 and 4 · CH2Cl2 are reported. Complex 4 is packed in the orthorhombic space group Pbca. The assembly of dichloromethane into the crystal lattice breaks the symmetry, whereby 4 · CH2Cl2 crystallises in the triclinic space group . In both modifications a square-planar palladium(II) ion is present, with the iodo atom and the Me(O)CS-C6H4 unit trans-positioned. The different crystal packing has no significant influence onto the geometry around the d8-configurated palladium atoms. 相似文献