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Nitrogenase Activity Associated with Roots and Stems of Field-Grown Corn (Zea mays L.) Plants
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Corn (Zea mays L.) plants were assayed for nitrogenase activity (C2H2 reduction) during early ear development. Hybrid corn and inbred lines were grown separately at two experimental fields in New Jersey. Acetylene-dependent ethylene production was observed a few hours after harvest, from the field, on intact plants, root-soil cores, lower stem segments, and excised roots, all assayed under air and not preincubated previously. Incubation of excised roots at 1% O2 resulted in lower rates of C2H2 reduction. The time course of C2H2 reduction by excised roots, assayed in air, was similar for all genotypes studied (two hybrids, eight inbreds, and a cross of corn × teosinte) and indicated that a long preincubation at reduced O2 is not absolutely required for early detection of nitrogenase activity. Isolation of N2-fixing bacteria from within the roots and stems, together with the diurnal fluctuation of nitrogenase activity in response to day/night cycles, were indicative of a close association with plant function. Collectively, the results provided strong evidence for the occurrence of nitrogenase activity associated with corn plants growing in a temperate climate and dependent upon indigenous N2-fixing bacteria. 相似文献
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Soil-dwelling mites of four plots under organic management were investigated in April and December 1998 and in December 1999. Their populations were compared with mite populations in a pasture and forest in the vicinity. It was observed that there was always an initial reduction in the populations of soil mites and in the activity of the epigeic forms whenever a plot was opened up and disturbed mechanically in preparation for cultivation, irrespective of previous organic inputs. With time, the densities and activities of mites recovered under organic management. The uropodine and oribatid mites in particular benefited more from organic management than gamasine and actinedid mites. Uropodine mites increased tremendously under banana where there was fresh cow dung manure. Oribatid mite species Nothrus seropedicalensis and Archegozetes magnus were dominant in organic plots where the soil was moist and temperatures were lower than the ambient. Protoribates rioensis was dominant in organic plots where the soil was drier and temperatures were higher than the ambient. Galumna was the most active oribatid taxon on the floor of all plots, with the highest activity recorded under maracuja and in pasture plots. The results suggest that while densities and activities of soil mites increased in the organic plots, the community structure and recruitment period of oribatid mites were altered. Oribatid mite diversity was higher in the organic plots than in the pasture but lower than in the forest, where Belba sp. and many Eremobelboid brachypiline genera were present, but absent in the organic plots and pasture. 相似文献
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Antonia G. T. Volpon Helvécio De-Polli Johanna Döbereiner 《Archives of microbiology》1981,128(4):371-375
Changes of cellular activities during batch cultures with Azospirillum lipoferum strain Br 17 (ATCC 29 709) were observed within the growth cycle, at optimal pO2 (0.002–0.003 atm). The relative growth rate for cells growing with N2 as sole nitrogen source during log phase was =0.13 h-1 and the doubling time was 5.3 h. Nitrogenase activity was not accompanied by hydrogen evolution at any growth stage, and a very active uptake hydrogenase was demonstrated. The hydrogenase activity increased towards the end of the growth period when glucose became limiting and N2 fixation reached its maximal specific activity. Oxygen consumption and oxygen tolerance at the various growth stages, increased simultaneously with the uptake hydrogenase activity indicating a possible role of this enzyme in an oxygen protection mechanism of A. lipoferum nitrogenase. The efficiency of nitrogen fixation expressed as mg total nitrogen fixed in cells and supernatant per g glucose consumed, was 20 at the early log phase and increased to 48 at the late log phase. About 25% of the total fixed nitrogen was recovered in the culture supernatant.Abbreviations DOT
Dissolved oxygen tension
- PHB
Poly--hydroxybutyric acid
- O.D.
Optical density (560 nm)
- A.T.C.C.
American type culture collection
- NTA
Nitrilotriacetic acid
Graduate student of the Universidade Federal Rural do Rio de Janeiro, Brazil 相似文献
4.
Spatial heterogeneity of soil respiration and related properties at the plant scale 总被引:17,自引:1,他引:16
Stoyan Helmut De-Polli Helvecio Böhm Sven Robertson G. Philip Paul Eldor A. 《Plant and Soil》2000,222(1-2):203-214
Geostatistical techniques were used to quantify the scale and degree of soil heterogeneity in 2 m2 plots around 9-year-old poplar trees and within a wheat field. Samples were taken during two years, on an unaligned grid,
for analysis of soil respiration, C and N content, available P, gravimetric moisture, pH, nitrification potential, and root
biomass. Kriged maps of soil respiration, moisture, and C content showed strong spatial structure associated with poplar trees
but not with wheat rows. All soil properties showed higher autocorrelation in June than in April. Isopleth patchiness for
all variates was less in June. This was associated with lower respiration rates due to lower litter decomposition. From the
degree and scale of heterogeneity seen in this study, we conclude that the main causes of soil heterogeneity at this scale
(2 m2) are likely to be found at micro scales controlled in part by plant root and plant residue patterns. These must be understood
in the evaluation of ecosystem processes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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