全文获取类型
收费全文 | 718篇 |
免费 | 92篇 |
专业分类
810篇 |
出版年
2022年 | 11篇 |
2021年 | 13篇 |
2019年 | 8篇 |
2018年 | 7篇 |
2016年 | 19篇 |
2015年 | 30篇 |
2014年 | 30篇 |
2013年 | 31篇 |
2012年 | 42篇 |
2011年 | 51篇 |
2010年 | 25篇 |
2009年 | 24篇 |
2008年 | 34篇 |
2007年 | 35篇 |
2006年 | 29篇 |
2005年 | 41篇 |
2004年 | 25篇 |
2003年 | 29篇 |
2002年 | 28篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 10篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1994年 | 11篇 |
1993年 | 4篇 |
1992年 | 10篇 |
1991年 | 17篇 |
1990年 | 9篇 |
1989年 | 12篇 |
1988年 | 21篇 |
1987年 | 6篇 |
1986年 | 11篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 10篇 |
1977年 | 7篇 |
1976年 | 11篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1973年 | 11篇 |
1972年 | 5篇 |
1970年 | 4篇 |
1965年 | 4篇 |
排序方式: 共有810条查询结果,搜索用时 15 毫秒
1.
Response of microbial populations to environmental disturbance 总被引:18,自引:0,他引:18
Taxonomic and genetic diversities of microbial communities disturbed by chemical pollutants were lower than in undisturbed
reference communities. The dominant populations within the disturbed communities had enhanced physiological tolerances and
substrate utilization capabilities, indicating that generalized physiological versatility is an adaptive characteristic of
populations that successfully compete within disturbed communities. 相似文献
2.
Y Yoshimura Y Hosoi S J Atlas A M Bongiovanni R Santulli E E Wallach 《Biology of reproduction》1986,35(4):943-948
The effects of aminoglutethimide phosphate (AGP) on ovulation, ovum maturation, fertilizability, and steroid production were studied with the use of an isolated perfused rabbit ovary preparation. AGP (10(-3) or 10(-4) M) was added to the perfusate of one ovary. The contralateral control ovary was perfused in medium alone. Thirty minutes later human chorionic gonadotropin (hCG) (50 IU) was added to the perfusate of all ovaries. No difference was observed in time of ovulation or ovulatory efficiency between controls and AGP-treated ovaries. The degree of ovum maturity and degeneration was also comparable in the two groups. Progesterone and estradiol production were significantly reduced by AGP treatment. A second experiment examined fertilizability of ova ovulated in vitro after perfusion with 10(-3) M AGP. AGP significantly reduced the rate of normal fertilization as observed 12 h after insemination. The percentage of inseminated ova with evidence of degeneration was greater in ova from AGP-treated ovaries than in those from controls, however, this difference was not significant. The study indicates that AGP affects neither hCG-induced ovulation nor meiotic resumption; however, fertilizability of ova from ovaries treated with AGP is impaired. These data suggest that the intrafollicular steroid environment may participate in cytoplasmic maturation of ovulated ova. 相似文献
3.
In this article we review the renal hemodynamic and excretory actions of atrial natriuretic factor (ANF) and consider some of the mechanisms of its vascular and natriuretic effects. ANF leads to a marked, sustained, and parallel increase in whole-organ and superficial single-nephron glomerular filtration rate (GFR) while mean blood pressure is decreased and renal blood flow (RBF) is unchanged or even decreased. The increase in GFR is caused by an efferent arteriolar vasoconstriction or by a combination of afferent vasodilation and efferent vasoconstriction. ANF also leads to a decrease in the hypertonicity of the innermedullary interstitium. Together with the increase in GFR, this phenomenon accounts wholly or in great part for the ANF-induced natriuresis. The overall renal vascular effects of ANF are complex and may tentatively be conceptualized as a behavior of a functional partial agonist: slight vasoconstriction in vasodilated kidneys, no sustained effects on the vascular resistance in normal kidneys, and vasodilation in vasoconstricted kidneys. The vasoconstrictor effect of ANF may be direct or indirect and depends on extracellular calcium whereas the antagonist effect likely results from alterations in intracellular calcium homeostasis. The data raise the perspective that ANF is not only a powerful natriuretic substance but has the potential of being an important modulator of GFR and RBF in intact animals. 相似文献
4.
Effects of atrial natriuretic factor on human platelet function 总被引:1,自引:0,他引:1
We examined the hypothesis that atrial natriuretic factor (ANF), a substance with known vasorelaxant activities, shares with other vasodilators the property of inhibiting platelet function. Aggregation of citrated platelet-rich plasma (PRP) from 23 healthy volunteers induced by ADP, adrenaline, arachidonic acid, collagen, gamma-thrombin, the endoperoxide analogue U-44069, serotonin, the calcium ionophore A-23187 or platelet aggregating factor was measured after incubation of PRP with ANF for 3 minutes at concentrations of 4 X 10(-9), 4 X 10(-8) and 4 X 10(-7) M or vehicle as control. ANF decreased ADP-induced aggregation significantly (P less than 0.02), but only at the highest concentration used and to a minor extent (control: 73.6 +/- 11.2%; after ANF 4 X 10(-7) M: 60.0 +/- 17.1%, mean +/- S.D., n = 39) by a selective inhibitory effect on the secondary wave; neither aggregation by all other agents tested nor thromboxane B2 generation induced by ADP and adrenaline was altered by incubation with ANF. Although ANF thus has detectable effects on ADP-induced platelet aggregation in vitro, these data suggest that ANF is unlikely to be a physiologically significant modulator of platelet function. 相似文献
5.
Daphne Gail Fautin 《Environmental Biology of Fishes》1986,15(3):171-180
Synopsis The 25 species ofAmphiprion and one ofPremnas (family Pomacentridae) are obligate symbionts of 10 species of facultatively symbiotic sea anemones. Throughout the tropical
Indo-West Pacific range of the relationship, a fish species inhabits only certain of the hosts potentially available to it.
This specificity is due to the fishes. Five fishes occupy six sea anemone species at Lizard Island, Great Barrier Reef, Australia.Entacmaea quadricolor harborsP. biaculeatus, A. melanopus andA. akindynos. Adults ofPremnas occur deeper than about 3 m in large, primarily solitary actinians; juveniles may occupy peripheral members ofEntacmaea clones in shallow water. Specimens ofA. melanopus live exclusively in clonal anemones, which are found no deeper than 3 m. Most individuals ofA. akindynos inEntacmaea are juveniles, occurring shallow and deep, in solitary anemones or at the margins of clones. Interspecific as well as intraspecific
social control of growth may be responsible for keeping fish small at clone fringes. Conspicuous specimens ofE. quadricolor depend upon their anemonefish to survive. Actinians cleared of symbionts disappeared within 24 h, probably having been eaten
by reef fishes.Entacmaea, the most abundant and widespread host actinian at Lizard Island and throughout the range of the association, is also arguably
the most attractive to anemonefishes. I believe its vulnerability to predation was a factor in its evolving whatever makes
it desirable to fishes. Experimental transfers pitted fish of one species against those of another, controlling for ecophenotype
of host, and sex, size and number of fish. Competitive superiority was in the same order as abundance and over-all host specificity:P. biaculeatus, A. melanopus, A. akindynos. At least three factors are necessary to explain patterns of species specificity - innate or learned host preference, competition,
and stochastic processes. 相似文献
6.
Hydrocarbon Metabolism by Brevibacterium erythrogenes: Normal and Branched Alkanes 总被引:6,自引:0,他引:6 下载免费PDF全文
Branched- and straight-chain alkanes are metabolized by Brevibacterium erythrogenes by means of two distinct pathways. Normal alkanes (e.g., n-pentadecane) are degraded, after terminal oxidation, by the beta-oxidation system operational in fatty acid catabolism. Branched alkanes like pristane (2,6,10,14-tetramethylpentadecane) and 2-methylundecane are degraded as dicarboxylic acids, which also undergo beta-oxidation. Pristane-derived intermediates are observed to accumulate, with time, as a series of dicarboxylic acids. This dicarboxylic acid pathway is not observed in the presence of normal alkanes. Release of (14)CO(2) from [1-(14)C]pristane is delayed, or entirely inhibited, in the presence of n-hexadecane, whereas CO(2) release from n-hexadecane remains unaffected. These results suggest an inducible dicarboxylic acid pathway for degradation of branched-chain alkanes. 相似文献
7.
The applicability of two methods (pyrolysis gas chromatography and acidification-wet oxidation) for determining14CO2 incorporation into soil microorganisms was investigated. Both methods were able to distinguish biologically incorporated14C from abiotically adsorbed14C, but to varying degrees, there being a larger carryover of abiotic14C into the organic fraction and a higher percentage of assimilated14C in the organic fraction with the wet oxidation method. Using14C assimilation as a measure, it was possible to determine microbial activities in soils of diverse properties under a variety
of conditions, including polar soils under harsh environmental conditions. Both light and dark14CO2 fixation was measurable.14CO2 assimilation was not always proportional to the enumerable microorganisms. A new design for measurement of microbial activityin situ enabled measurement of total C influx (primary productivity) into soils with minimal perturbation to the natural soil ecosystem. 相似文献
8.
9.
Donald H. Williamson Malcolm J. Gardner Peter Preiser Daphne J. Moore Kaveri Rangachari Robert J. M. Wilson 《Molecular & general genetics : MGG》1994,243(2):249-252
In common with other Apicomplexan parasites, Plasmodium falciparum carries two extrachromosomal DNAs, one of which, the 6 kb element, is undoubtedly mitochondrial. The second, generally referred to as the 35 kb circle, is of unknown provenance, but the nature and organization of its genetic content makes a mitochondrial association unlikely and the molecule has features reminiscent of plastid genomes. We now report the occurrence on the circle of an open reading frame specifying a predicted 470 amino acid protein that shares more than 50% identity with a gene currently known only on the plastome of red algae. This high degree of conservation confirms the 35 kb circle's plastid ancestry, and we speculate that it may have originated from the rhodoplast of an ancient red algal endosymbiont in the progenitor of the Apicomplexa. 相似文献
10.
Extended models of the ventilatory response to sustained isocapnic hypoxia in humans 总被引:2,自引:0,他引:2
Liang, Pei-Ji, Daphne A. Bascom, and Peter A. Robbins.Extended models of the ventilatory response to sustained isocapnic hypoxia in humans. J. Appl. Physiol. 82(2): 667-677, 1997.The purpose of this study was to examine extensions of a modelof hypoxic ventilatory decline (HVD) in humans. In the original model (model I) devised by R. Painter, S. Khamnei, and P. Robbins(J. Appl. Physiol. 74: 2007-2015, 1993), HVD is modeledentirely by a modulation of peripheral chemoreflex sensitivity. In thefirst extension (model II), a more complicated dynamic is usedfor the change in peripheral chemoreflex sensitivity. In the secondextension (model III), HVD is modeled as a combination ofboth the mechanism of Painter et al. and a component that isindependent of peripheral chemoreflex sensitivity. In all cases, aparallel noise structure was incorporated to describe the stochasticproperties of the ventilatory behavior to remove the correlation of theresiduals. Data came from six subjects from a study by D. A. Bascom, J. J. Pandit, I. D. Clement, and P. A. Robbins (Respir. Physiol.88: 299-312, 1992). For model II, there was a significantimprovement in fit for two out of six subjects. The reasons for thiswere not entirely clear. For model III, the fit was againsignificantly improved in two subjects, but in this case the subjectswere those who had the most marked undershoot and recovery ofventilation at the relief of hypoxia. In these two subjects, thechemoreflex-independent component contributed ~50% to total HVD.In the other four subjects, the chemoreflex-independent componentcontributed ~10% to total HVD. It is concluded that in somesubjects, but not in others, there may be a component of HVD thatis independent of peripheral chemoreflex sensitivity. 相似文献