Erratum to: MYH7B variants cause hypertrophic cardiomyopathy by activating the CaMK-signaling pathway

Science China Life Sciences -     

Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco

A histidine kinase-based signaling system has been proposed to function in ethylene signal transduction pathway of plants and one ethylene receptor has been found to possess His kinase activity. Here we demonstrate that a His kinase-like ethylene receptor homologue NTHK1 from tobacco has serine/threonine (Ser/Thr) kinase activity, but no His kinase activity. Evidence obtained by analyzing acid/base stability, phosphoamino acid and substrate specificity of the phosphorylated kinase domain, supports this conclusion. In addition, mutation of the presumptive phosphorylation site His (H378) to Gln did not affect the kinase activity whereas deletion of the ATP-binding domain eliminated it, indicating that the conserved His (H378) is not required for the kinase activity and this activity is intrinsic to the NTHK1-KD. Moreover, confocal analysis of NTHK1 expression in insect cells and plant cells suggested the plasma membrane localization of the NTHK1 protein. Thus, NTHK1 may represent a distinct Ser/Thr kinase-type ethylene receptor and function in an alternative mechanism for ethylene signal transduction.     

乌拉尔图小麦(Triticum urartu)1Ay高分子量麦谷蛋白亚基基因编码区的克隆与鉴定

应用简并性引物和基因组PCR反应从乌拉尔图小麦(Triticum urartu)不同种质材料中获得并测定了表达型和沉默型1Ay高分子量麦谷蛋白亚基基因全长编码区的基因组DNA序列.表达型1Ay基因编码区的序列与前人已发表的y型高分子量麦谷蛋白亚基基因编码区的序列高度同源,由其推导的1Ay亚基的一级结构与已知的高分子量麦谷蛋白亚基相似.在细菌细胞中,表达型1Ay基因编码区的克隆序列可经诱导而产生1Ay蛋白,该蛋白与种子中1Ay亚基在电泳迁移率和抗原性上类似,表明所克隆的序列真实地代表了表达型1Ay基因的全长编码区.但是,本研究所克隆的沉默型1Av基因的编码区序列因含有3个提前终止子而不能翻译成完整的1Ay蛋白.讨论了表达型1Ay基因在小麦籽粒加工品质改良中的潜在利用价值以及lAy基因沉默的机制.     

环形电极介导的小麦基因转化

用环形电极电激法有效地将外源DNA导入完整的小麦幼胚组织中。电激的物理参数采用770V／cm场强、800μF电容、100μg／mL质粒DNA(含有bar和GUS双标记基因),幼胚被电激3次。经PCR和Southern杂交分析表明,外源基因已稳定整合到小麦基因组中,转化频率为7．5％,高于相同处理条件下基因枪法的转化频率(4．2％)。     

一种转录受病原物接种和机械创伤诱导的水稻WRKY基因的鉴定

WRKY基因编码一类植物特异性转录因子,该类基因目前在双子叶植物中得到了较好的研究.为了研究WRKY基因在单子叶植物中的生物学功能,从水稻(Oryza sativa L.)中分离了OsiWRKY基因的cDNA.该cDNA可编码一个含482个氨基酸残基的蛋白质,该蛋白质预测的一级结构中含有一个WRKY结构域,在该结构域中发现了一个典型的C2-H2锌指结构模块.OsiWRKY预测的一级结构中还可能含有一个细胞核定位因子;利用瞬时表达体系,发现OsiWRKY与GFP绿色荧光蛋白(GFP)的融合蛋白的确定位在水稻细胞核中.在凝胶阻滞实验中,体外表达的OsiWRKY蛋白可特异地结合W-box顺式因子.在水稻白叶枯病原细菌接种实验中,OsiWRKy基因的转录本在抗病品种(IR-26)中的诱导增加强度明显高于感病品种(Jingang 30).在模拟接种的水稻中,OsiWRKy基因的转录本也表现诱导增加,但其诱导增加的起始时间和幅度要显著低于水稻白叶枯病原细菌接种的水稻植株.这些结果表明,OsiWRKY基因可能编码了一个含有WRKY结构域的转录因子,该转录因子可能参与了水稻对病原物接种和机械创伤的反应,但OsiWRKY蛋白调节两种反应的机制可能有所不同.     

Genomic distribution of quantitative trait loci for yield and yield-related traits in common wheat

A major objective of quantitative trait locus(QTL)studies is to find genes/markers that can be used in breeding programs via marker assisted selection(MAS).We surveyed the QTLs for yield and yield related traits and their genomic distributions in common wheat(Triticum aestivum L.)in the available published reports.We then carried out a meta-QTL(MQTL)analysis to identify the major and consistent QTLs for these traits.In total,55 MQTLs were identified,of which 12 significant MQTLs were located on wheat chromosomes 1A,1B,2A,2D,3B,4A,4B,4D and 5A.Our study showed that the genetic control of yield and its components in common wheat involved the important genes such as Rht and Vrn.Furthermore,several significant MQTLs were found in the chromosomal regions corresponding to several rice genomic locations containing important QTLs for yield related traits.Our results demonstrate that meta-QTL analysis is a powerful tool for confirming the major and stable QTLs and refining their chromosomal positions in common wheat,which may be useful for improving the MAS efficiency of yield related traits.     

中国人扩张型心肌病与受磷蛋白基因关系的研究

受磷蛋白（phospholamban）是心肌收缩的一个重要调节因子,可抑制心肌肌浆网钙ATPase的活性、降低其对钙的亲和力。正常情况下,受磷蛋白可被不同的蛋白激酶磷酸化从而解除其肌浆网钙ATPase的抑制作用。国外两个DCM家系研究发现受磷蛋白基因突变与DCM的发生有关,研究目的旨在探讨中国人群心肌特异性受磷蛋白基因突变与特发性扩张型心肌病发病的关系。收集60例确诊的特发性扩张型心肌病患者临床资料,采集血样本并分离白细胞和提取基因组DNA。应用PCR扩增肌特异性受磷蛋白基因片段,经测序检测基因突变的存在。结果显示60例扩张型心肌病人受磷蛋白基因开放阅读框未发现突变,仅有两例患者出现阅读框3′部分非翻译区分别离终止密码153和173位单个碱基T的缺失。两例患者终止密码后非翻译区单个碱基T的缺失据分析并无实际意义。因此我们认为绝大部分中国人扩张型心肌病的发病可能与受磷蛋白基因突变无关。Abstract: Phospholamban (PLB) is a prominent regulator of myocardial contractility and a reversible inhibitor of the cardiac sarcoplasmic reticulum Ca2+ ATPase activity. In normal cardiac muscles, phospholamban can be phosphorylated at distinct sites by various protein kinases and release its inhibition to sarcoplasmic reticulum Ca2+ ATPase. The studies of pedigrees have shown dilated cardiomyopathy (DCM) is related with mutation of PLB. The aim of present study is to investigate the relationship between mutation of PLB gene and DCM. Sixty patients with idiotic DCM were enrolled in present study. The clinical data were collected, including clinical symptoms, ECG and echocardiography. Peripheral blood samples of all these subjects were collected to extract genome DNA. The fragments of PLB gene were amplified by PCR and PCR fragment sequencing was performed to study weather mutation of phospholamban gene exists. phospholamban gene did not show any mutation in these patients. Most Chinese DCM patients may not be related with mutation of PLB gene.     

Creating Wheat Germplasm for High Quality Breeding by Monosomic Backcrossing

By crossing bread wheat cultlvar GC8901 with the 1D monosonlc line of Xiaoyan No. 6 and backcrosslng the offsprlng with the Xlaoyan No. 6 1D monosonlc llne for 5 years, high-molecular-welght glutenin subunlts 1Dx5＋1Dy10 from GC8901 have been transferred Into wheat cultivar Xiaoyan No. 6. The BC5F1 offspring lines had been detected by using methods of cytology, marker, molecular marker and six elite single plants with high molecular-welght glutenin subunlts： lAx1, 1Bx14＋1 By15, 1Dx5＋1 Dy10 were Identified. Those lines have high-yleld potential with better agronomic characters and have been used In high quality wheat breeding processes as well.     

Decreased serum albumin level indicates poor prognosis of COVID-19 patients: hepatic injury analysis from 2,623 hospitalized cases

Science China Life Sciences - Coronavirus disease 2019 (COVID-19) is a global pandemic which has caused numerous deaths worldwide. The present study investigated the roles of hypoproteinemia in the...