Hepatic Insulin-Positive Cells and Major Transcription Factors (PDX1, MAFA,NGN3) in Rat Models of Type 1 and Type 2 Diabetes Mellitus

Journal of Evolutionary Biochemistry and Physiology - Due to a high occurrence of diabetes mellitus (DM) and its complications, insulin-positive cells detected in different organs are of great...     

Mechanism of the suppression of the spinal pain syndrome by serotonin derivatives

The ability of serotonin derivatives to stimulate cAMP accumulation in isolated nerve terminals and lumbar enlargement of the spinal cord of normal rats was compared. The effect of the compounds on the intensity of spinal pain syndrome was also assessed. It has been established that substitutes injected into NH2-group of serotonin in 5-OH position attenuate the ability to stimulate cAMP accumulation in synaptosomes, with the effect more pronounced with substitutes of larger volume. A certain correlation between the ability of serotonin derivatives to stimulate adenylate cyclase in vivo and in vitro, on the one hand, and their analgetic effect, on the other hand, is suggested.     

The Effects of a Low-Frequency Electric Field on Recombinant Luciferase Activity

Biophysics - Studies on the effects of an electromagnetic field on the activity of recombinant luciferase in the luciferase–luciferin–ATP-Mg2+ system were conducted. The enzymatic...     

Inhibitory effect of the amygdala on alimentary-conditioned reflexes in the dog

In experiments on dogs, changes of conditioned alimentary reflexes were studied under stimulation (50-100 mcA, 1, 40, 100 imp/s, 0.5 ms) of the basolateral area of amygdala before and after electrolytic ablation (5 mA, 60 s) of the head of the caudate nucleus. Stimulation of the amygdala, which elicited inhibition of conditioned secretory reflexes and prolongation of latencies in intact dogs leads to opposite - activating - influences after destruction of the caudate nucleus head. A conclusion is made on the participation of neurophysiological and neurochemical mechanisms of the caudate nucleus in transmission of inhibitory effects of the amygdala on alimentary conditioned reflexes.     

Active immunization to exogenous and endogenous antidepressants. The formation of antibodies to biogenic amines and peptide regulators

The active immunization of rabbits and white rats to antidepressant sydnophen results in the formation of antibodies binding norepinephrine, dophamine, serotonine as well peptide regulatory compounds: substance P, pynorphine, vasopressin and beta-endorphin. The immunization against endogenic antidepressant thyroliberin induces the formation of antibodies to the same biogenic amines and to the gamma-aminobutyric acid, oxytocin and leu encephalin. The data obtained are discussed in connection with some physiological and biochemical changes found earlier during immunization to antidepressants.     

Formation of temporary connections in dogs following destruction of the amygdaloid complex, head of the caudate nucleus and orbital cortex]

Ablation of the orbital cortical zones in dogs after ablation of the basolateral part of the amygdala and the caudate nucleus head considerably impeded the elaboration of secretory conditioned alimentary reflexes, without affecting the rate of elaboration of simple instrumental food-procuring reaction. The dogs lost however the capacity to differentiate non-reinforced stimuli from reinforced ones, which was revealed in both secretory and instrumental reactions.     

Ultrastructure of the silkworm testis. II. Ductus deferens]

Ultrastructure of the silkworm's ducts defferens is described after spermatozoa went out of lobulli testis and after the end of secretion. D. defferens is composed of an external layer of the ring and longitudinal muscles and of an internal layer of glandular cells. Both layers are separated by structurless lamina--tunica propria. Z-discs of irregular form are placed at the borders of sarcomeres. M-lines are absent. Sarcolemma intrudes into the muscle fibers at the level of Z-discs and gives origin to T-system the tubes of which together with sarcoplasmic reticulum form dyades. Structures like intercalate discs are observed in muscle tissue at the level of Z-discs. Glandular cells have unusually developed nucleoli, many ribosomes, lysosome-like and residual bodies. There are comb desmosomes between the glandular cells.     

The role of the orbital cortex in feeding behavior]

It has been found that extirpation of the orbital zones of the cerebral cortex in dogs in which the structures of the limbic and striate levels of nervous integration were previously ablated, produced more profound disturbances of the higher nervous activity than the previous operations. Not only conditioned positive and inhibitory reactions were affected, but unconditioned alimentary reflexes as well, while food motivation sharply decreased. Further experiments on the operated animals and could go on, only when alimentary excitability was enhanced. A conclusion has been drawn that the orbital zones of the cerebral cortex are directly related to the achievement of an integrated alimentary behavioral reaction.