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G. Cassinelli E. Cotta G. D'Amico C. Della Bruna A. Grein R. Mazzoleni M. L. Ricciardi R. Tintinelli 《Archives of microbiology》1970,70(3):197-210
Summary A new microorganism, isolated in our laboratories and identified as Streptomyces michiganensis var. amylolyticus var. nova is described.Thaimycins can be obtained by submerged fermentation of this microorganism on a suitable culture medium.Three new related compounds, thaimycins A, B and C have been obtained and characterized by their physical and chemical properties.Data on the antiprotozoal and anthelmintic activities in vitro as well as in vivo are reported. 相似文献
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Summary NAAG is one of the neuropeptides found in highest concentrations in the CNS. The presence of micromolar concentrations of NAAG in human CSF was demonstrated by using two different and complementary analytical approaches: 1) isocratic separation of endogenous NAAG by reverse-phase high performance liquid chromatography (HPLC) with dual wavelength detection and 2) derivatization of endogenous NAAG with acidic methanol and subsequent HPLC analysis of the derivative NAAG-trimethyl ester. The NAAG concentration was between 0.44µmol/l and 7.16µmol/l (mean of 2.19 ± 1.53µmol/l) in CSF samples from forty neuropsychiatric patients. Endogenous NAAG or [3H]NAAG added to CSF samples were not significantly degraded when the CSF was incubated at 37°C during one hour, suggesting that the peptide is a highly stable metabolite in the subarachnoid space. In addition, evidence is provided that NAAG does not present a concentration gradient along the lower subarachnoid space. 相似文献
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L. M. Ricciardi 《Biological cybernetics》1976,24(4):237-240
A generalization of an earlier paper (Capocelli and Ricciardi, 1971), dealing with a diffusion approximation for a neuron subject to one excitatory and one inhibitory Poisson input, is provided by not imposing any restrictions on number and magnitude if synaptic inputs. An equation for the neuron's transition p.d.f. is derived, use of which is made to determine the moments of the membrane potential. It is finally shown that a diffusion approximation is possible and that the resulting diffusion process is characterized by constant infinitesimal variance and linear drift. 相似文献
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Bacteriocins produced by lactic acid bacteria are a heterogeneous group of peptide inhibitors which include lantibiotics
(class I, e.g. nisin), small heat-stable peptides (class II, e.g. pediocin AcH/PA1) and large heat-labile proteins (class
III, e.g. helveticin J). Many bacteriocins belonging to the first two groups can be successfully used to inhibit undesirable
microorganisms in foods, but only nisin is produced industrially and is licensed for use as a food preservative in a partially
purified form. This review focuses on the production and purification of class I and class II bacteriocins from lactic acid
bacteria. Bacteriocin production is growth associated but the yield of bacteriocin per unit biomass is affected by several
factors, including the producing strain, media (carbohydrate and nitrogen sources, cations, etc.) and fermentation conditions
(pH, temperature, agitation, aeration and dilution rate in continuous fermentations). Continuous fermentation processes with
cell recycle or immobilized cells can result in a dramatic improvement in productivity over batch fermentations. Several simple
recovery processes, based on adsorbing bacteriocin on resins or silica compounds, have been developed and can be used to build
integrated production processes.
Received: 29 December 1998 / Received revision: 23 April 1999 / Accepted: 23 April 1999 相似文献
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Flavio I. Bachini Danilo Pereira Ruan Santos Matheus Hausen Glauber Pereira Camila Vieira Lee Taylor Marcelle Pegurier 《Biology of sport / Institute of Sport》2022,39(3):745
Capillary dried blood spot (DBS) samples facilitate field-based collection without venipuncture. This pilot study aims to evaluate the viability of creatine (Cr) and creatinine (Crt) quantification using fresh capillary serum (CrS/CrtS) and DBS samples (CrDBS/CrtDBS), using Flow Injection Analysis Mass Spectrometry (FIA – MS). Nine Olympic Athletes provided a capillary blood sample to assess CrS/CrtS and CrDBS/CrtDBS quantified by FIA – MS. No difference between CrtS (mean ± SD: 813.6 ± 102.4 μmol/L) and CrtDBS (812.4 ± 108.1 μmol/L) was observed with acceptable variance [SEM 88.7; CV 10.7%; ICC 0.57 (CI 95% 0.06 – 0.84)] and agreement [very strong (Spearman: r = 0.77; p < 0.01) or strong (Pearson: r = 0.56; p = 0.04); Bland Altman: lower (-193) and upper (+196) limits of agreement]. CrS (mean ± SD: 691.8 ± 165.2 μmol/L) was significantly different to CrDBS (2911 ± 571.4 μmol/L) with unacceptable variance [SEM 171.6; CV 27%; ICC 0.002 (CI 95% -0.02 – 0.07)] and ‘weak’ agreement [Spearman: r = 0.21, p = 0.47 and Pearson: r = 0.06, p = 0.84; Bland Altman lower (-3367) and upper (-1072) limits of agreement]. Crt quantification is viable using both CrtS and CrtDBS (but not for Cr and CrS/CrDBS), with the DBS tissue handling technique offering several methodological and practice facing advantages. Future work should expand upon the sample size, explore sport/discipline relevant analytes across a full competitive season, including key training, recovery and performance blocks of their periodized performance plan. 相似文献
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Shivange AV Serwe A Dennig A Roccatano D Haefner S Schwaneberg U 《Applied microbiology and biotechnology》2012,95(2):405-418
Phytase improves as a feed supplement the nutritional quality of phytate-rich diets (e.g., cereal grains, legumes, and oilseeds) by hydrolyzing indigestible phytate (myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate) and increasing abdominal absorption of inorganic phosphates, minerals, and trace elements. Directed phytase evolution was reported for improving industrial relevant properties such as thermostability (pelleting process) or activity. In this study, we report the cloning, characterization, and directed evolution of the Yersinia mollaretii phytase (Ymphytase). Ymphytase has a tetrameric structure with positive cooperativity (Hill coefficient was 2.3) and a specific activity of 1,073?U/mg which is ~10 times higher than widely used fungal phytases. High-throughput prescreening methods using filter papers or 384-well microtiter plates were developed. Precise subsequent screening for thermostable and active phytase variants was performed by combining absorbance and fluorescence-based detection system in 96-well microtiter plates. Directed evolution yielded after mutant library generation (SeSaM method) and two-step screening (in total ~8,400 clones) a phytase variant with ~20% improved thermostability (58°C for 20?min; residual activity wild type ~34%; variant ~53%) and increased melting temperature (1.5°C) with a slight loss of specific activity (993?U/mg). 相似文献
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