Methods for the isolation, sterilization and cultivation of Lemnaceae

SUMMARY. A method is described for isolating and sterilizing fronds of Lemnà gibba, L. minor, L. trisulca, L. polyrrhiza and Wolffia arrhiza , employing enrichment followed by sodium hypochlorite treatment. The method enables about 4% of the total number of treated fronds to be used as initiators of clonal cultures in which bacteria responding to organic enrichment and algae are excluded. Appropriate growth media, culture vessels and culture conditions are detailed.     

Limnochemical and phytoplankton studies on Nyumba ya Mungu reservoir, Tanzania

This publication reports on the chemical and phytoplankton aspects of a three month biological survey of Nyumba ya Mungu reservoir, Tanzania.
The lake had a total salt concentration of about 8 meq dm^-3 and specific conductivity of 900 μS cm^-1. The main salt in solution was sodium bicarbonate, and when the lake level fell it sometimes formed a crust on the exposed shore. The concentrations of major nutrient ions were probably not limiting to algal growth and good nutrient replenishment was provided by the two inflow rivers.
Nyumba ya Mungu supports a rich phytoplanton dominated by Melosira and blue-green algae. Acetone extractions gave chlorophyll 'a' concentrations ranging from 20–40 mgm^-3 in the vertical profiles, and light and dark bottle experiments indicated a maximum gross photosynthesis of about 800 mg O₂ m^-3h^-1. There was evidence of chemical stratification in the open water and diurnal stratification in the sheltered bays, but discontinuities were short-lived and the Trade Winds ensured regular mixing of the water. The lake appeared to have a higher primary production than the larger Rift Valley lakes and it was considered that this level of production would continue.     

Lakes of south-western Uganda

The vegetation of Lake Bunyonyi, a high altitude lake in Kigezi, S.W. Uganda, is described. The lake had two distinct plant communities. In the shallow sheltered bays and inflows extensive floating swamps of Cyperus papyrus L. and Cladium janiaicen. se Cranz. existed. On the steep, exposed shores there was only a narrow band of vegetation, dominated by firmly rooted Phragmites australis (Cav.) Steud., usually with papyrus behind. Floating-leaved Potamogeton and Nymphaea sometimes occurred outside the emergent species, and submerged species, including Ceratophyllum demersum L. grew down to 8 m. The pattern of vegetation, and the contribution of floating islands to the distribution of plants in the lake is discussed. Susceptibility to fragmentation, and distribution by wind are thought to be major factors governing the pattern. Oxygen proflies measured at the fringe of swamps indicated that the swamps did not cause appreciable deoxygenation of the water.     

Lakes of south-western Uganda

Lake Bunyonyi was formed about 18,000 years B.P. by a volcanic eruption which blocked a steep-sided valley. Earlier this century fish were introduced into the lake but recently there have been mass fish deaths. Although the thermocline was inconspicuous, density profiles showed the lake to the stratified and chemical evidence suggested stratification was for many years. The water was clear with little plankton and the epilimnioncontained low concentrations of dissolved mineral ions. The hypolimnion was anoxic and had a relatively high mineral ion concentration. The mass fish deaths were attributed to a violent shallow mixing, probably caused by wind, but mixing was insufficient to remineralize the epilimnion.     

The periphyton communities of Nyumba ya Mungu reservoir, Tanzania

The periphyton communities established in Nyumba ya Mungu were investigated between July and September 1974.
One hundred and ninety-two taxa were identified. One hundred and fifteen taxa were collected in the southern end of the lake, where 97 taxa were non-selectively distributed in the littorine epiphytic, epilithic, and epixylic environments. The offshore epixylon supported only 53 taxa. The epipelon and epipsammon were poorly developed at the southern end of the lake but were well developed in the northern inflow regions.
Diatoms and cyanophytes were the dominant phyla which contributed over 80% of the algal numbers. Chlorophytes were not common. Taxonomic compositions of periphyton on artificial and natural surfaces were similar, but artificial substrata accumulated greater proportional representations of diatoms.
The mean net production rates of periphyton on artificial substrata located at depths from 0-0.65 m for 28 days ranged from 140 mg organic dry weight m^-2 day^-1 on shoots in the macrophyte zone to 820 mg organic dry weight m^-2 day^-1 on glass surfaces on a rocky shore. The epiphytic environments produced the least standing crops of less than 250, 000 individuals cm^-2, but maximum densities of over 2,000,000 individuals cm^-2 were recorded in the epilithon.     

Nyumba ya Mungu reservoir, Tanzania The general features

Nyumba ya Mungu reservoir was completed in 1965 in northern Tanzania. By 1970 there was a thriving tilapia fishery but it declined catastrophically in subsequent years. A team of biologists surveyed the lake in 1974, and this paper outlines their hydrological, geographical, and climatic findings.
The dam, constructed across a north/south Neogene fault-trough 80 km south of Mt. Kilimanjaro, has produced a reservoir about 180 km² with a mean depth of 6 m. The catchment area is extensive but the main source of water is from Mt. Kilimanjaro. The lake is situated in an arid area, and over one quarter of the input water evaporates from the lake's surface. The outflow is regulated by a hydro-electric power station and is practically constant. At full capacity the retention time of the lake is one year but due to abnormally low rainfall, the reservoir was not full, and the retention time was nearer nine months.
The lake is polymictic and a deep wind-driven current is maintained by the Trade Winds for most of the year.
The general features of Nyumba ya Mungu are briefly compared with other man-made and natural lakes in Africa.