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The effect of sucrose, H2BO3, KNO3, Ca(NO2)2.4H2O and MgSO4.7H2O on pollen germination of Phytolacca dodecandra L. (endod)in a liquid medium was investigated. Sucrose and H3BO3 werecritical to pollen germination. A concentration of 10% sucroseand 161.8 µm H2BO3 gave over 70% germination. The germinationof pollen was not enhanced by Ca(NO3)2.4H2O, KNO3 and MgSO4.7H2O.Endod pollen was dehydrated over CaCl2 and stored in gelatincapsules in cryogenic vials at 175 °C, 1±1°C and 24±2 °C. The pollen moisture content atcollection was approx. 7.8% (f. wt basis) and dehydration overCaCl2 reduced it to about 1.4%. Pollen stored at 1±1°C and 175 °C maintained viability for over 6months. Pollen stored at room temperature lost viability within4 weeks of storage. Pollination with cryopreserved pollen resultedin normal fruit set. Phytolacca dodecandra, endod, pollen germination, pollen storage 相似文献
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