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Monacelli Barbara Pasqua Gabriella Cuteri Angelina Vitali Alberto 《Plant Cell, Tissue and Organ Culture》1999,58(2):81-85
A protocol for in vitro plant regeneration through organogenesis was established for Vismia guianensis(Hypericaceae), a species that produces an anti-cancer compound. The highest mean number of shoots per gram of callus (57.33)
was obtained on Murashige and Skoog medium supplemented with 4.44 μM 6-benzyl-aminopurine, 5.70 μM indole-3-acetic acid and
12.88 μM gibberellic acid. Rooting was favoured by the addition of 10 μM indole-3-butyric acid, and by sucrose concentrations
higher than 1%.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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G. Pasqua B. Monacelli A. Cuteri F. Spuntarelli N. Rascio B. Botta G. Delle Monache R. Scurria 《Protoplasma》1995,189(1-2):9-16
Summary The accumulation and tissue localization of antitumoral vismione A in the in vitro regenerated plants ofVismia guianensis DC. were investigated. Chemical and light and electron-microscope analyses revealed that vismione A, detected as phenolic black globules in the vacuoles, was accumulated in the leaf, mainly in the palisade, and in small amounts in the primary body of the stem (epidermis and first cortical layer). Vismione A is neither present in the secretory cavities and ducts of the leaf nor in the secretory ducts of the stem. In the leaves of the regenerated plants, the amount of vismione A reached 0.5% FW, compared to 0.1% in the leaves of the parent plant. The optimization of the in vitro regeneration of plants was obtained in MS medium enriched with BAP (1 ppm). The best results for the rooting of regenerated plants were achieved with MS medium containing half-strength salts and 10–5 MIBA.Abbreviations APT
attached proton test
- BAP
6-benzyl-aminopurine
- CC
column chromatography
- 2,4-D
2,4-dichlorophenoxyacetic acid
- EtOAc
ethyl acetate
- IBA
indole-3-butyric acid
- MeOH
methanol
- MS
Murashige and Skoog
- NAA
-naphthaleneacetic acid
- NMR
nuclear magnetic resonance
- TEM
transmission electron microscopy
- TLC
thin layer chromatography
- UV
ultraviolet 相似文献
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