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Doumit Camilios Neto Joel Alexandre Meira Elaine Tiburtius Patrício Peralta Zamora Cryshelen Bugay David Alexander Mitchell Nadia Krieger Dr. 《Biotechnology journal》2009,4(5):748-755
Pseudomonas aeruginosa UFPEDA 614 produced a rhamnolipid biosurfactant when grown on sugarcane bagasse impregnated with a solution containing glycerol. Biosurfactant levels reached 40 g of rhamnolipid per kilogram of dry initial substrate after 12 days. On the basis of the volume of liquid used, the biosurfactant levels were similar to those obtained in submerged liquid culture of a medium identical to the impregnating solution. The properties of the biosurfactant were very similar to those obtained with rhamnolipids produced in submerged culture, with a critical micelle concentration of 46.8 mg/L and an emulsification index at 24 h of over 90% against gasoline. The surface properties were maintained after autoclaving of the fermented solids, meaning that it is possible to minimize safety risks by killing the producing organism with a heat treatment of the solids prior to product extraction. The biosurfactant was used in the washing of soils contaminated with gasoline. An aqueous biosurfactant solution was 3.2-fold more efficient than water in leaching organic material from the soil, demonstrating the viability of application of rhamnolipids in the bioremediation of soils contaminated with gasoline. 相似文献
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Camilios-Neto D Bugay C de Santana-Filho AP Joslin T de Souza LM Sassaki GL Mitchell DA Krieger N 《Applied microbiology and biotechnology》2011,89(5):1395-1403
Rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological
acceptability. However, production in submerged culture is made difficult by severe foaming problems. Solid-state cultivation
(SSC) is a promising alternative production method. In the current work, we report the optimization of rhamnolipid production
by Pseudomonas aeruginosa UFPEDA 614 on a solid substrate containing sugarcane bagasse and corn bran. The best rhamnolipid production, 45 g/l of impregnating
solution used, was obtained with a 50:50 (m/m) mixture of sugarcane bagasse and corn bran supplemented with an impregnating
solution containing 6% (v/v) of each of glycerol and soybean oil. This level is comparable with those of previous studies
undertaken in solid-state cultivation; the composition of the biosurfactant is similar, but our medium is cheaper. Our work
therefore provides a suitable basis for future studies of the development of an SSC-based process for rhamnolipid production. 相似文献
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