全文获取类型
收费全文 | 404篇 |
免费 | 60篇 |
专业分类
464篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 5篇 |
2016年 | 11篇 |
2015年 | 14篇 |
2014年 | 18篇 |
2013年 | 23篇 |
2012年 | 23篇 |
2011年 | 38篇 |
2010年 | 19篇 |
2009年 | 22篇 |
2008年 | 32篇 |
2007年 | 23篇 |
2006年 | 21篇 |
2005年 | 15篇 |
2004年 | 18篇 |
2003年 | 17篇 |
2002年 | 8篇 |
2001年 | 15篇 |
2000年 | 12篇 |
1999年 | 14篇 |
1998年 | 6篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 6篇 |
1989年 | 9篇 |
1988年 | 9篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 1篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有464条查询结果,搜索用时 0 毫秒
1.
2.
Sergio Davinelli Mariano Intrieri Claudio Russo Alfonso Di Costanzo Davide Zella Paolo Bosco Giovanni Scapagnini 《Immunity & ageing : I & A》2011,8(1):1-10
Alzheimer's disease is a progressive and neurodegenerative disorder which involves multiple molecular mechanisms. Intense research during the last years has accumulated a large body of data and the search for sensitive and specific biomarkers has undergone a rapid evolution. However, the diagnosis remains problematic and the current tests do not accurately detect the process leading to neurodegeneration. Biomarkers discovery and validation are considered the key aspects to support clinical diagnosis and provide discriminatory power between different stages of the disorder. A considerable challenge is to integrate different types of data from new potent approach to reach a common interpretation and replicate the findings across studies and populations. Furthermore, long-term clinical follow-up and combined analysis of several biomarkers are among the most promising perspectives to diagnose and manage the disease. The present review will focus on the recent published data providing an updated overview of the main achievements in the genetic and biochemical research of the Alzheimer's disease. We also discuss the latest and most significant results that will help to define a specific disease signature whose validity might be clinically relevant for future AD diagnosis. 相似文献
3.
4.
The typical tissue isoferritin pattern varies during neoplastic transformation, usually shifting toward a more acidic profile. To investigate the molecular basis of this phenomenon, we have analyzed the steady-state levels of the H and L mRNAs in several neoplastic tissues. By using specific probes for the two ferritin subunits, we have found, in three different adenocarcinomas and in a case of Hodgkin lymphoma, a two- to four-fold increase of the H and L mRNA levels compared to those found in normal human liver. 相似文献
5.
At least two nuclear gene products are specifically required for translation of a single yeast mitochondrial mRNA. 总被引:25,自引:4,他引:21 下载免费PDF全文
Mitochondrial translation of the oxi2 mRNA, encoding yeast cytochrome c oxidase subunit III (coxIII), has previously been shown to specifically require the mitochondrially located protein product of the nuclear gene PET494. We show here that this specific translational activation involves at least one other newly identified gene termed PET54. Mutations in PET54 cause an absence of the coxIII protein despite the presence of normal levels of its mRNA. pet494 mutations are known to be suppressible by mitochondrial gene rearrangements that replace the normal 5'-untranslated leader of the oxi2 mRNA with the leaders of other mitochondrial mRNAs. In this study we show that pet54, pet494 double mutants are suppressed by the same mitochondrial gene rearrangements, showing that the PET54 product is specifically required, in addition to the PET494 protein, for translation of the oxi2 mRNA. Since, as we show here, PET54 is not an activator of PET494 gene expression, our results suggest that the products of both of these genes may act together to stimulate coxIII translation. 相似文献
6.
7.
Cloning and sequencing of a full length cDNA coding for human retinol-binding protein. 总被引:12,自引:3,他引:9 下载免费PDF全文
V Colantuoni V Romano G Bensi C Santoro F Costanzo G Raugei R Cortese 《Nucleic acids research》1983,11(22):7769-7776
We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that the gene is present in one or few copies per haploid genome and is transcribed in a single mRNA species. 相似文献
8.
Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA. 总被引:10,自引:3,他引:7 下载免费PDF全文
G Raugei G Bensi V Colantuoni V Romano C Santoro F Costanzo R Cortese 《Nucleic acids research》1983,11(17):5811-5819
We have isolated and sequenced a cDNA clone coding for human haptoglobin. Our sequence shows that haptoglobin is very likely synthesized as a single polypeptide chain which is then cleaved at an Arg residue to generate its two characteristic alpha and beta subunit. Southern blot analysis suggests that there are at least two copies of the haptoglobin gene per haploid genome. 相似文献
9.
Brain Cell Biology - The olfactory epithelium of the adult hamster (Mesocricetus auratus) was studied using the scanning electron microscope. A method that produced fractures in the epithelium... 相似文献
10.
Brain Cell Biology - The olfactory epithelium of the adult hamster (Mesocricetus auratus) was examined with the scanning electron microscope following olfactory nerve axotomy. Axotomy results in... 相似文献