全文获取类型
收费全文 | 261篇 |
免费 | 78篇 |
出版年
2023年 | 4篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 8篇 |
2014年 | 9篇 |
2013年 | 10篇 |
2012年 | 11篇 |
2011年 | 7篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 16篇 |
2007年 | 11篇 |
2006年 | 12篇 |
2005年 | 13篇 |
2004年 | 3篇 |
2003年 | 8篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 10篇 |
1998年 | 8篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1992年 | 9篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 2篇 |
1983年 | 11篇 |
1982年 | 7篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1968年 | 3篇 |
1965年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有339条查询结果,搜索用时 15 毫秒
1.
2.
3.
Heinz W. Kunz Andrea L. Cortese Hassett Tetsuo Inomata D. N. Misra Thomas J. Gill III 《Immunogenetics》1989,30(3):181-187
A new antigenic system in the rat homologous to theQa/TL antigen system in the mouse has been characterized. It was detected by antibodies raised in donor-recipient combinations
that were matched for theRT1. A, B, D, E loci in the major histocompatibility complex (MHC): (R11×BN)F1 anti-BN.1L(LEW), (R18×BN)F1 anti-BN.1L, and BN.1LV1(F344) anti-BN.1L. Absorption analyses using these antisera and a variety of inbred, congenic and
recombinant strains identified three alleles,RT1.G
a
,G
b
,G
c
, of whichG
c
is a null allele. The strain distribution of these alleles was determined, using 37 strains of rats representative of all
of the prototypic haplotypes and a number of congenic and recombinant strains. The use of the congenic and recombinant strains
showed that theRT1.G locus was linked to the MHC and that the most probable gene order wasA-E-G. Testcross analysis showed that the map distance betweenA andG was 1.4 cM(4/285 recombinants). The RT1.G antigen has a heavy chain ofM
r 46 000 and is present on both T and B cells. 相似文献
4.
5.
6.
Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor. 总被引:8,自引:5,他引:3
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
D Kardassis M Hadzopoulou-Cladaras D P Ramji R Cortese V I Zannis C Cladaras 《Molecular and cellular biology》1990,10(6):2653-2659
7.
Generation of small mutation in large genomic fragments by homologous recombination: description of the technique and examples of its use 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Nucleic acids research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M Tripodi S Perfumo R Ali L Amicone C Abbott R Cortese 《Nucleic acids research》1990,18(21):6247-6251
We have developed a technique of homologous recombination in bacteria which allows the mutagenesis of large genomic fragments cloned in cosmids. The desired mutation is first introduced into a plasmid clone and is then transferred to the appropriate cosmid clone by the means of double antibiotic selection coupled with phenotypic selection. We describe three different types of construct made by this technique. 相似文献
8.
The alpha-like globin gene cluster in rabbits contains embryonic zeta-
globin genes, an adult alpha-globin gene, and theta-globin genes of
undetermined function. The basic arrangement of genes, deduced from
analysis of cloned DNA fragments, is 5'-zeta 0-zeta 1-alpha 1-theta 1- zeta
2-zeta 3-theta 2-3'. However, the pattern of restriction fragments
containing zeta- and theta-globin genes varies among individual rabbits.
Analysis of BamHI fragments of genomic DNA from 24 New Zealand white
rabbits revealed eight different patterns of fragments containing
zeta-globin genes. The large BamHI fragments containing genes zeta 0 and
zeta 1 are polymorphic in length, whereas a 1.9-kb fragment containing the
zeta 2 gene and the 3.5-kb fragment containing the zeta 3 gene do not vary
in size. In contrast to this constancy in the size of the restriction
fragments, the copy number of the zeta 2 and zeta 3 genes does vary among
different rabbits. No length polymorphism was detected in the BamHI
fragments containing the theta-globin genes, but again the copy number
varies for restriction fragments containing the theta 2 gene. The alpha 1-
and theta 1-globin genes are located in a nonpolymorphic 7.2-kb BamHI
fragment. The combined data from hybridization with both zeta and theta
probes shows that the BamHI cleavage pattern does not vary within the
region 5'-alpha 1-theta 1- zeta 2-zeta 3-theta 2-3', but the pattern
genomic blot-hybridization patterns for the progeny of parental rabbits
with different zeta-globin gene patterns shows that the polymorphic
patterns are inherited in a Mendelian fashion. Two different haplotypes
have been mapped based on the genomic blot-hybridization data. The
variation in the alpha-like globin gene cluster in the rabbit population
results both from differences in the copy number of the duplication block
containing the zeta-zeta-theta gene set and from the presence or absence of
polymorphic BamHI sites.
相似文献
9.
10.