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1.
The speed and accuracy of protein synthesis are fundamental parameters for understanding the fitness of living cells, the quality control of translation, and the evolution of ribosomes. In this study, we analyse the speed and accuracy of the decoding step under conditions reproducing the high speed of translation in vivo. We show that error frequency is close to 10−3, consistent with the values measured in vivo. Selectivity is predominantly due to the differences in kcat values for cognate and near-cognate reactions, whereas the intrinsic affinity differences are not used for tRNA discrimination. Thus, the ribosome seems to be optimized towards high speed of translation at the cost of fidelity. Competition with near- and non-cognate ternary complexes reduces the rate of GTP hydrolysis in the cognate ternary complex, but does not appreciably affect the rate-limiting tRNA accommodation step. The GTP hydrolysis step is crucial for the optimization of both the speed and accuracy, which explains the necessity for the trade-off between the two fundamental parameters of translation. 相似文献
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Abstract Pseudomonas sp. strain RW611 utilized the ammonium salt of 2-sulfobenzoate as sole source of carbon, sulfur, nitrogen, and energy. The xenobiotic sulfo substituent was dioxygenolytically eliminated as sulfite, which was then slowly oxidized to sulfate. 2,3-Dihydroxybenzoate, which resulted from desulfonation underwent meta -cleavage, mediated by 2,3-dihydroxybenzoate 3,4-dioxygenase activity. This enzyme was inhibited by 3-chlorocatechol and 2,3,4-trihydroxybenzoate. 相似文献
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A new species of Mallomonas, M. nuussuaqensis was found to be common in samples collected from waterbodies on Nuussuaq/Nûgssuaq (70N-71N, West Greenland). The species has very compact silica scales with a thick broad marginal rim and a very thick and broad hood, which often occupies more than two thirds of the shield. 相似文献
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Søren Wilken Rasmussen 《Chromosoma》1977,60(3):205-221
In Bombyx mori oocytes the synaptonemal complexes are retained in modified form from pachytene to metaphase I. At the end of pachytene the length and width of the lateral components of the complex increase, whereafter the complexes become compacted during later stages of the meiotic prophase. Ultimately, at metaphase I the modified synaptonemal complexes of individual bivalents fuse to form a more or less continuous sheet between the homologous chromosomes. This sheet corresponds to the structure historically known as the elimination chromatin. It is concluded that in the absence of crossing over and chiasma formation in Bombyx mori females the retainment and subsequent modification of the synaptonemal complex has evolved as a substitute mechanism to ensure regular disjunction of the bivalents. 相似文献
8.
Porosity, Eh and interstitital nitrogen compounds profiles are given for four locations in the eastern Skagerrak. Sediments down to 30 cm depth, i.e. postindustrial in age, were analysed. The results together with a detailed age determination (210Pb) suggest a high degree of both lateral and vertical variability as a result of local bottom topography and currents as well as event-controlled sedimentation processes. 相似文献
9.
Pollen morphology of 14 species of Collomia (Polemoniaceae) was examined by light microscopy, and by both scanning and transmission electron microscopy. Four distinct pollen types were observed which are based principally upon 1) shape, number and distribution of apertures, and 2) surface sculpturing: Type 1—zonocolporate with striate ridges; Type 2—zonocolporate with striato-reticulate ridges; Type 3—pantoporate with radiate ridges; Type 4—pantoporate with irregularly reticulate ridges. Evaluation of pollen morphology reveals considerable discrepancy with respect to presently accepted sectional classification. Collomia grandiflora of sect. Collomia has a pollen type similar to that of members of sect. Collomiastrum and is now interpreted as representing an independent evolutionary line derived from the latter section. Collomia diversifolia of sect. Courtoisia has a pollen morphology similar to that of sect. Collomia. whereas C. heterophylla of the same section possesses pollen unique within the genus. This last pollen type shows close similarity to the pollen of members of Polemonium, Gilia, Leptodactylon, and Ipomopsis. Pollen of C. tinctoria and C. tracyi of sect. Collomia are anomalous within Polemoniaceae. No significant difference in exine stratification was discernible among the four pollen types. 相似文献
10.
Dieter H. Wilken 《American journal of botany》1978,65(8):896-901
Thirty-seven characters were measured on greenhouse grown progenies of twenty-three populations representing, for Collomia linearis, a broad geographical and ecological distribution in western North America. Twelve vegetative and seventeen floral characters showed significant among-population/within-population variance ratios (F-statistics). Overall, floral characters showed higher F-statistics than did vegetative characters. The F-statistics and character means were used to construct phenograms based on weighted similarity measures and single-linkage clustering. With the exception of a homogeneous cluster comprising northern Rocky Mountain OTUs, relationships based on floral characters were geographically random. No clearly defined morphological trends toward increased autogamy were observed in association with geographical or ecological distribution. Several clustering relationships, based on vegetative characters, were interpreted as representing geographically unrelated genotypes convergent with respect to ecotypic differentiation. The most distinctive clusters of OTUs in the vegetative phenogram were associated with subalpine and wet meadow sites, habitats considered as ecologically marginal for the species. Comparison of the two phenograms revealed considerable disparity between relationships based on floral characters and those based on vegetative characters. This disparity lends support to the hypothesis that floral and vegetative character combinations are subject to independent selective processes. The absence of polyploidy or mitotically detectable chromosomal rearrangements indicates that the observed variation among widely separated populations represents either divergence or convergence via genetic differentiation at the diploid level. 相似文献