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Christoforos A. Hadjistasi Ioannis J. Stavrou Raluca‐Ioana Stefan‐Van Staden Hassan Y. Aboul‐Enein Constantina P. Kapnissi‐Christodoulou 《Chirality》2013,25(9):556-560
In this study, simple electrophoretic methods were developed for the chiral separation of the clinically important compounds fucose and pipecolic acid. In recent years, these analytes, and particularly their individual enantiomers, have attracted considerable attention due to their role in biological functions and disorders. The detectability and sensitivity of pipecolic acid and fucose were improved by reacting them with fluorenylmethyloxycarbonyl chloride (FMOC‐Cl) and 5‐amino‐2‐naphthalene‐sulfonic acid (ANSA), respectively. The enantioseparation conditions were optimized by initially investigating the type of the chiral selector. Different chiral selectors, such as polymeric surfactants and cyclodextrins, were used and the most effective ones were determined with regard to resolution and analysis time. A 10‐mM β‐cyclodextrin was able to separate the enantiomers of ANSA‐DL‐fucose and the polymeric surfactant poly(sodium N‐undecanoyl‐LL‐leucine‐valinate) was able to separate the enantiomers of FMOC‐DL‐pipecolic acid, with resolution values of 3.45 and 2.78, respectively. Additional parameters, such as the concentration and the pH of the background electrolyte (BGE), the concentration of the chiral selector, and the addition of modifiers were examined in order to optimize the separations. The addition of the chiral ionic liquid D‐alanine tert‐butyl ester lactate into the BGE was also investigated, for the first time, in order to improve resolution of the enantiomers. Chirality 25:556–560, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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Bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. Vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus Vibrio mostly entering larval rearing water through live feeds, such as Artemia and rotifers. The pathogenic Vibrio alginolyticus strain V1, isolated during a vibriosis outbreak in cultured seabream, Sparus aurata, was used as host to isolate and characterize the two novel bacteriophages φSt2 and φGrn1 for phage therapy application. In vitro cell lysis experiments were performed against the bacterial host V. alginolyticus strain V1 but also against 12 presumptive Vibrio strains originating from live prey Artemia salina cultures indicating the strong lytic efficacy of the 2 phages. In vivo administration of the phage cocktail, φSt2 and φGrn1, at MOI = 100 directly on live prey A. salina cultures, led to a 93% decrease of presumptive Vibrio population after 4 h of treatment. Current study suggests that administration of φSt2 and φGrn1 to live preys could selectively reduce Vibrio load in fish hatcheries. Innovative and environmental friendly solutions against bacterial diseases are more than necessary and phage therapy is one of them. 相似文献
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Yahya Elsayed Constantina Lekakou Paul Tomlins 《Biotechnology and bioengineering》2019,116(6):1509-1522
The paper presents a transient, continuum, two-phase model of the tissue engineering in fibrous scaffolds, including transport equations for the flowing culture medium, nutrient and cell concentration with transverse and in-plane diffusion and cell migration, a novel feature of local in-plane transport across a phenomenological pore and innovative layer-by-layer cell filling approach. The model is successfully validated for the smooth muscle cell tissue engineering of a vascular graft using crosslinked, electrospun gelatin fiber scaffolds for both static and dynamic cell culture, the latter in a dynamic bioreactor with a rotating shaft on which the tubular scaffold is attached. Parametric studies evaluate the impact of the scaffold microstructure, cell dynamics, oxygen transport, and static or dynamic conditions on the rate and extent of cell proliferation and depth of oxygen accessibility. An optimized scaffold of 75% dry porosity is proposed that can be tissue engineered into a viable and still fully oxygenated graft of the tunica media of the coronary artery within 2 days in the dynamic bioreactor. Such scaffold also matches the mechanical properties of the tunica media of the human coronary artery and the suture retention strength of a saphenous vein, often used as a coronary artery graft. 相似文献
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Lundwall A Band V Blaber M Clements JA Courty Y Diamandis EP Fritz H Lilja H Malm J Maltais LJ Olsson AY Petraki C Scorilas A Sotiropoulou G Stenman UH Stephan C Talieri M Yousef GM 《Biological chemistry》2006,387(6):637-641
The human kallikrein locus on chromosome 19q13.3-13.4 contains kallikrein 1--the tissue kallikrein--and 14 related serine proteases. Recent investigations into their function and evolution have indicated that the present nomenclature for these proteins is inadequate or insufficient. Here we present a new nomenclature in which proteins without proven kininogenase activity are denoted kallikrein-related peptidase. Names are also given to the unique rodent proteins that are closely related to kallikrein 1. 相似文献
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Constantina Heltianu Simona-Adriana Manea Cristian Guja Carina Mihai Constantin Ionescu-Tirgoviste 《Central European Journal of Biology》2008,3(3):243-249
Advanced glycation end products (AGEs) are involved in the occurrence of vascular complications in diabetes. The present study
was undertaken to investigate the level of low-molecular weight products of AGEs (LMW-AGEs) in relation to microvascular complications
in type 1 diabetes, and the possible relationship with nitric oxide (NO) as a marker of endothelial function. Patients with
normal renal function (NRF) were classified into two groups: (1) without, and (2) with diabetic neuropathy; and patients with
renal impairment also into two groups: (3) diabetic renal disease, and (4) end-stage renal disease. The fluorescence of LMW-AGEs
and measurement of NO metabolites was assessed in 277 serum samples. In addition, multiple regression analysis was performed.
In group 1, LMW-AGEs level (9.3±1.1 AF%) was higher than in the control group (2.4±0.3 AF%). A trend in the increase of LMW-AGEs
with neuropathy (29.7±5.5 AF%, group 2), and further with renal impairment (47.0±8.0, group 3 and 137.8±25.5 AF%, group 4),
was observed. In multivariate regression analysis LMW-AGEs were associated with NO metabolites in group 2. In NRF patients,
diabetic neuropathy was significantly correlated with LMW-AGEs and NO metabolites, independently of serum creatinine and duration
of diabetes. This relationship suggests that the NO and LMW-AGEs’ actions (possibly synergistic) in endothelial activation
possess a role in the initiation and development of diabetic microvascular complications. 相似文献
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The fate of hydrophobic xenobiotic pollutants such as linear alkylbenzene sulfonates (LAS), nonylphenol ethoxylates (NPEO) and di-ethyl-hexyl phthalate (DEHP) during sewage sludge composting was addressed in this work. The experiments were conducted in a fully automated in-vessel autothermal composting system which was fed with a mixture of primary and secondary sludge and manure. The mixture composition was determined to achieve satisfactory humidity, C/N ratio and free air space (FAS). The effect of various parameters, such as the initial xenobiotic concentration, the presence of multiple xenobiotic compounds and the temperature of composting material sustained during the process on the xenobiotics biodegradation kinetics was investigated. It was generally established that significant xenobiotic reduction is achievable through composting under all conditions tested. According to the obtained results, the presence of LAS, NPEO and DEHP even at higher concentrations was not inhibitory to the bioprocess. However, the presence of multiple xenobiotic compounds such as NPEO, NP and DEHP in the sludge can influence LAS removal during LAS composting. 相似文献
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Using the non-ionic detergent dodecyl--D-maltoside we have developed a preparative method for the isolation of the 43 kDa, 47 kDa and D1-D2-Cyt b
559 species directly from thylakoid membranes. In contrast to previous procedures the photosynthetic membrane was exposed only to one mild detergent and that resulted in more stable preparations. The isolated species were examined spectroscopically and it was found that even under these mild conditions the D1-D2-Cyt b
559 did not retain the primary quinone QA.Abbreviations PS II
Photosystem II
- CF
Coupling Factor
- LHC
Light Harvesting Complex
- MES
4-morpholine-ethanesulfonic acid
- BIS-TRIS
2-[Bis (2-hydroxyethyl) amino]-2-hydroxymethyl-propane-1,3-diol
- SMN
0.4 M sucrose/50 mM MES (pH6)/10 mM NaCl 相似文献
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