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1.
Occurrence of a capsule in Aeromonas salmonicida 总被引:3,自引:0,他引:3
Antonieta Garrote Ramon Bonet Susana Merino Maria Dolors Simon-Pujol Francisco Congregado 《FEMS microbiology letters》1992,95(2-3):127-131
Aeromonas salmonicida grown in a medium with excess glucose as carbon source produces both capsular and exocellular polysaccharides. The capsular polysaccharide is composed of glucose, mannose, rhamnose, N-acetylmannosamine and mannuronic acid in the molar ratios of approximately 5:3:0.75:2:1. The extracellular polysaccharide is similarly constituted, but in the molar ratios of approximately 4.75:10.5:1.5:2:1. The capsular and exocellular polysaccharides did not cross-react with monoclonal antibodies against the A-layer or the O-antigen lipopolysaccharide. 相似文献
2.
Inma Estañol M.Dolores Simon-Pujol Francisco Congregado 《FEMS microbiology letters》1989,60(3):295-298
Abstract The effect of the inorganic phosphate concentration on the activity of the enzyme of alternate peripheral pathways of glucose catabolism was studied in Pseudomonas cepacia ATCC 17759. Growth with low glucose concentration (0.5% w/v) and 20 mM phosphate resulted in induced levels of the phosphorylative pathway enzymes when compared with the levels of these same enzymes in high glucose concentration (2% w/v). However, an expansion of the oxidative pathway was detected during growth with 0.5% (w/v) of glucose and high phosphate concentration (160 mM). Moreover, under high phosphate (160 mM) and high glucose (2% w/v) growth conditions, glucokinase activity was increased preferentially relative to levels of direct oxidative pathway enzymes. 相似文献
3.
Ramón Bonet Beatriz Magariños Jesús L. Romalde Maria Dolores Simon-Pujol Alicia E. Toranzo Francisco Congregado 《FEMS microbiology letters》1994,124(3):285-289
Abstract Pasteurella piscicida grown in a glucose-rich medium produces a capsule that can be see under light and electron microscopy. The capsular polysaccharide was purified and characterized by chemical and HPLC analysis. The polymer has the composition glucose/mannose/ N -acetylgalactosamine/galacturonic acid/acetic acid in the molar ratios of approximately 2.5:1.3:0.5:0.4:2.5. The polysaccharide was immunogenic in rabbits and did not cross-react with antibodies against the O-antigen lipopolysaccharide. 相似文献
4.
Congregado F. Estañol I. Espuny M. J. Fusté M. C. Manresa M. A. Marqués A. M. Guinea J. Simon-Pujol M. D. 《Biotechnology letters》1985,7(12):883-888
Summary Exopolysaccharide production byPseudomonas sp. strain EPS-5028 increased under conditions of high C/N ratios at the pH range 6.7–8 and 25°C. The polymer was formed from a variety of carbon substrates and contains D-glucose, D-galactose and uronic acid in the approximate molar ratio of 325. The polysaccharide produced is of high viscosity (1,600 cP; 1% polymer in D. I. water). 相似文献
5.
Santiago LLovera Ramon Bonet Maria Dolores Simon-Pujol Francisco Congregado 《Applied microbiology and biotechnology》1993,39(3):424-426
The influence of some ions in pre-growth culture medium on chromate reduction by resting cells of Agrobacterium radiobacter strain EPS-916 was investigated. The reduction was dependent on the Fe2+ content of the culture medium: the higher the iron content, the lower the reduction rate. The cells showed maximum chromate reduction when pre-grown in the presence of 0.243 m Mg2+, 20 m Ca2+ and 3.6 m Mn2+. Chromate reduction was not affected by the addition of MgCl2, CdCl2, ZnCl2, MnCl2, Na2SO4 (1000 m), and Na2MoO4 (100 m) to the activity assays. However, activity was inhibited by the presence of Na2SO4 (10 mm), Na2MoO4 (200 m) and ferric citrate. 相似文献
6.
Effect of two organophosphorus insecticides on the phosphate-dissolving soil bacteria. 总被引:1,自引:1,他引:0
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Dimethoate and malathion added to soil at 10 and 100 microgram/g caused an initial stimulation of CO2 production. Total counts of bacterial propagules were increased. All insecticide applications increased bacteria producing phospholipases from week 1 until week 4 after the application; bacteria then returned to the original levels. 相似文献
7.
Ana María Marqués Ramon Bonet María Dolores Simon-Pujol María Carmen Fusté Francisco Congregado 《Applied microbiology and biotechnology》1990,34(3):429-431
Summary Accumulation of uranium (U) is reported for isolated exopolysaccharide produced by Pseudomonas sp. EPS-5028. A maximum uptake of 96 g U/mg polymer was observed. In contrast, the maximum accumulation of uranium by deacylated polysaccharide was 46 g/mg. This metal-complexing capacity observed suggests that the anionic reactive sites on the structure could be responsible for this activity. Metal uptake was affected by pH and was not affected by temperature. Expolysaccharide from Pseudomonas sp. EPS-5028 obeyed the Freundlich isotherm indicating single layer adsorption.
Offprint requests to: F. Congregado 相似文献
8.
Ana M. Marqués Xavier Roca M. Dolores Simon-Pujol M. Carmen Fuste Francisco Congregado 《Applied microbiology and biotechnology》1991,35(3):406-410
Summary
Pseudomonas sp. EPS-5028 was examined for the ability to accumulate uranium from solutions. The uptake of uranium by this microorganism is very rapid and is affected by pH but not by temperature, metabolic inhibitors, culture time and the presence of various cations and anions. The amount of uranium absorbed by the cells increased as the uranium concentration of the solution increased up to 55 mg uranium/g cell dry weight. Electron microscopy indicated that uranium accumulated intracellularly as needle-like fibrils. Uranium could be removed chemically from the cells, which could then be reused as a biosorbent.
Offprint requests to: A. M. Marqués 相似文献
9.
Production and Rheological Properties of the Extracellular Polysaccharide Synthesized by Pseudomonas sp. Strain EPS-5028 总被引:1,自引:0,他引:1
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Ana M. Marqus Inmaculada Estaol Joan M. Alsina Carmen Fust Dolores Simon-Pujol Jesús Guinea Francisco Congregado 《Applied microbiology》1986,52(5):1221-1223
During batch aerobic submerged fermentation, the exopolysaccharide synthesis by Pseudomonas sp. strain EPS-5028 occurred in growth- and non-growth-linked processes. Polysaccharide formation increased when the pH was controlled at 7 during fermentation. Exopolysaccharide production depended on the phosphate content of the medium. The polymer exhibited a pseudoplastic nature, had good thermostability, and was affected neither by pH nor by high concentrations of salt. 相似文献
10.
M.A. Manresa M.C. Fusté A.M. Marqués F. Congregado M. D. Simon-Pujol J. Guinea 《Biotechnology letters》1986,8(2):91-94
Summary
E. coli CF3 isolated after the infection ofE. coli K12 C600 with the bacteriophage B278 produces quantities of extracellular exopoly-saccharide characterized by gas chromatography as composed of glucose, galactose and fucose in the molar ratio 111.2 and being partly acetylated. Solutions of the polysaccharide were pseudoplastic and its viscosity is constant at extreme pH (3.5–10) and compatible with temperature (90°C). 相似文献