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C Crone J Frokjaer-Jensen JJ Friedman O Christensen 《The Journal of general physiology》1978,71(2):195-220
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Miller TA White ED Kinley KA Congleton JJ Clark MJ 《Journal of strength and conditioning research / National Strength & Conditioning Association》2002,16(1):44-49
Performance data for 261 NCAA Division 1A collegiate football players were analyzed to determine if player position, body weight, body fat, and training time were correlated with changes in performance in the following events: power clean (PC), bench press (BP), squat (SQ), vertical jump (VJ), 40-yd dash (40yd), and 20-yd shuttle (20yd). Individual positions were combined into the following groups: (A) wide receivers, defensive backs, and running backs, (B) linebackers, kickers, tight ends, quarterbacks, and specialists, and (C) linemen. Increases in body weight were positively correlated with increases in BP and PC performance for all groups. Increases in body fat were negatively correlated with performance in the PC and VJ for all groups. For group C, increases in body fat were also negatively correlated with performance in the 40yd and 20yd. Group and training time exhibited no linear relationship with performance in any of the tested events. No linear relationships were observed between the independent variables and performance in the SQ. When individual training data were analyzed longitudinally, a nonlinear increase in performance in the PC, BP, and SQ was observed as training time increased, with the greatest rate of change occurring between the first and second semesters of training. 相似文献
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Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG
Claes Ingmar JJ Segers Marijke E Verhoeven Tine LA Dusselier Michiel Sels Bert F De Keersmaecker Sigrid CJ Vanderleyden Jos Lebeer Sarah 《Microbial cell factories》2012,11(1):1-8
Background
Receptors with a single transmembrane (TM) domain are essential for the signal transduction across the cell membrane. NMR spectroscopy is a powerful tool to study structure of the single TM domain. The expression and purification of a TM domain in Escherichia coli (E.coli) is challenging due to its small molecular weight. Although ketosteroid isomerase (KSI) is a commonly used affinity tag for expression and purification of short peptides, KSI tag needs to be removed with the toxic reagent cyanogen bromide (CNBr).Result
The purification of the TM domain of p75 neurotrophin receptor using a KSI tag with the introduction of a thrombin cleavage site is described herein. The recombinant fusion protein was refolded into micelles and was cleaved with thrombin. Studies showed that purified protein could be used for structural study using NMR spectroscopy.Conclusions
These results provide another strategy for obtaining a single TM domain for structural studies without using toxic chemical digestion or acid to remove the fusion tag. The purified TM domain of p75 neurotrophin receptor will be useful for structural studies. 相似文献10.