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Elif Yavuz Hatice Gunes Sebnem Harsa Cisem Bulut A. Fazil yenidunya 《World journal of microbiology & biotechnology》2004,20(8):871-874
Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles. 相似文献
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Yavuz Elif Gunes Hatice Bulut Cisem Harsa Sebnem Yenidunya A. Fazil 《World journal of microbiology & biotechnology》2004,20(6):535-537
The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment
length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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