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Ann M Hess Abhishek N Prasad Andrey Ptitsyn Gregory D Ebel Ken E Olson Catalin Barbacioru Cinna Monighetti Corey L Campbell 《BMC microbiology》2011,11(1):45
Background
Small RNA (sRNA) regulatory pathways (SRRPs) are important to anti-viral defence in mosquitoes. To identify critical features of the virus infection process in Dengue serotype 2 (DENV2)-infected Ae. aegypti, we deep-sequenced small non-coding RNAs. Triplicate biological replicates were used so that rigorous statistical metrics could be applied. 相似文献2.
Brian B. Tuch Rebecca R. Laborde Xing Xu Jian Gu Christina B. Chung Cinna K. Monighetti Sarah J. Stanley Kerry D. Olsen Jan L. Kasperbauer Eric J. Moore Adam J. Broomer Ruoying Tan Pius M. Brzoska Matthew W. Muller Asim S. Siddiqui Yan W. Asmann Yongming Sun Scott Kuersten Melissa A. Barker Francisco M. De La Vega David I. Smith 《PloS one》2010,5(2)
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Christina A. Bormann Chung Victoria L. Boyd Kevin J. McKernan Yutao Fu Cinna Monighetti Heather E. Peckham Melissa Barker 《PloS one》2010,5(2)
Methylation, the addition of methyl groups to cytosine (C), plays an important role in the regulation of gene expression in both normal and dysfunctional cells. During bisulfite conversion and subsequent PCR amplification, unmethylated Cs are converted into thymine (T), while methylated Cs will not be converted. Sequencing of this bisulfite-treated DNA permits the detection of methylation at specific sites. Through the introduction of next-generation sequencing technologies (NGS) simultaneous analysis of methylation motifs in multiple regions provides the opportunity for hypothesis-free study of the entire methylome. Here we present a whole methylome sequencing study that compares two different bisulfite conversion methods (in solution versus in gel), utilizing the high throughput of the SOLiD™ System. Advantages and disadvantages of the two different bisulfite conversion methods for constructing sequencing libraries are discussed. Furthermore, the application of the SOLiD™ bisulfite sequencing to larger and more complex genomes is shown with preliminary in silico created bisulfite converted reads. 相似文献
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