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Molecular Biology - Hypertrophic cardiomyopathy (HCM) is the most common genetically determined heart pathology and is often accompanied by fatal complications. Today, the traditional view of the... 相似文献
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Polushin N Malykh A Malykh O Zenkova M Chumakova N Vlassov V Kozyavkin S 《Nucleosides, nucleotides & nucleic acids》2001,20(4-7):507-514
Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented. 相似文献
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Kedishvili NY Chumakova OV Chetyrkin SV Belyaeva OV Lapshina EA Lin DW Matsumura M Nelson PS 《The Journal of biological chemistry》2002,277(32):28909-28915
All-trans-retinoic acid is a metabolite of vitamin A (all-trans-retinol) that functions as an activating ligand for a family of nuclear retinoic acid receptors. The intracellular levels of retinoic acid in tissues are tightly regulated, although the mechanisms underlying the control of retinoid metabolism at the level of specific enzymes are not completely understood. In this report we present the first characterization of the retinoid substrate specificity of a novel short-chain dehydrogenase/reductase (SDR) encoded by RalR1/PSDR1, a cDNA recently isolated from the human prostate (Lin, B., White, J. T., Ferguson, C., Wang, S., Vessella, R., Bumgarner, R., True, L. D., Hood, L., and Nelson, P. S. (2001) Cancer Res. 61, 1611-1618). We demonstrate that RalR1 exhibits an oxidoreductive catalytic activity toward retinoids, but not steroids, with at least an 800-fold lower apparent K(m) values for NADP+ and NADPH versus NAD+ and NADH as cofactors. The enzyme is approximately 50-fold more efficient for the reduction of all-trans-retinal than for the oxidation of all-trans-retinol. Importantly, RalR1 reduces all-trans-retinal in the presence of a 10-fold molar excess of cellular retinol-binding protein type I, which is believed to sequester all-trans-retinal from nonspecific enzymes. As shown by immunostaining of human prostate and LNCaP cells with monoclonal anti-RalR1 antibodies, the enzyme is highly expressed in the epithelial cell layer of human prostate and localizes to the endoplasmic reticulum. The enzymatic properties and expression pattern of RalR1 in prostate epithelium suggest that it might play a role in the regulation of retinoid homeostasis in human prostate. 相似文献
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M. A. Zenkova N. L. Chumakova A. V. Vlassov N. I. Komarova A. G. Venyaminova V. V. Vlassov V. N. Silnikov 《Molecular Biology》2000,34(3):390-394
The mechanism of hydrolysis of RNA substrates—diribonucleoside monophosphate CpA and decaribonucleotide UUCAUGUAAA—by chemical
constructs functionally mimicking ribonuclease A was studied. It is shown that RNA cleavage by chemical RNases 2L2 and 2D3
proceeds similar to the RNase A-induced RNA hydrolysis through 2′,3′-cyclophosphate as an intermediate product. A comparison
of hydrolyses of CpA in water and D2O revealed an isotope effect (K
H/K
D=2.28), which implies acid-base catalysis at the limiting stage of the reaction. Two feasible mechanisms of RNA hydrolysis
by chemical RNases (linear and adjacent) are discussed. 相似文献
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The present paper concerns 4 groups of haemolymph cells of fleas (proleukocytes, leukocytes, trophic cells and oenocytoids), results of observations on their phagocytic activity during parenteral infection of insects with bacteria, bacilli, and cells' response to the infection with Microsporidia. 相似文献
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Blagodatskikh KA Evdokimova MA Agapkina IuV Nikitin AG Brovkin AN Pushkov AA Blagodatskikh EG Kudriasheva OIu Osmolovskaia VS Minushkina LO Kochkina MS Selezneva ND Dankovtseva EN Chumakova OS Baklanova TN Talyzin PA Reznichenko NE Donetskaia OP Tereshchenko SN Krasil'nikova ES Dzhaiani NA Akatova EV Glezer MG Galiavich AS Zakirova VB Kaziolova NA Timofeeva IV Iagoda AV Boeva OI Katel'nitskaia LI Khorolets EV Shlyk SV Volkova ÉG Margarian MP Guz' OI Konstantinov VO Timofeeva NV Sidorenko BA 《Molekuliarnaia biologiia》2010,44(5):839-846
We investigated the association of gene IL6 G(-174)C polymorphism and gene IL10 G(-1082)A polymorphism with coronary artery disease (CAD) in the Russian population. A total of 1145 patients with CAD diagnose on the basis of clinical studies in cardiological hospitals of Moscow, St -Petersburg, Kazan, Chelyabinsk, Perm, Stavropol and Rostov-on-Don. Supervision term was 9.10 +/- 5.03 months (the maximum term 18 months). In case of gene IL10 G(-1082)A polymorphism we determined that patients with CAD diagnose and A alleles gene IL10 had unfavorable outcome more often than patients with homozygous G alleles. Survival time from end point from carrier genotype GA and AA is 11.68 +/- 0.67 months against 12.69 +/- 0.65 months from carrier phenotype GG gene IL10 (chi2 = 4.13, p = 0.042). The group studied do not differ significantly with respect to the distributions of gene IL6 G(-174)C alleles and genotypes. However in case combined group studies of gene IL10 G(-1082)A polymorphism and IL6 G(-174)C polymorphism we determined that patients with CAD diagnose and carrier genotype GG gene IL6 and genotype GA and AA gene IL10 had unfavorable outcome more often (survival time 11.01 +/- 1.24 months) than patients with genotype CC and CG gene IL6 and genotype GG gene IL10 (survival time 13.28 +/- 0.83 months) chi2 = 10.23, p = 0.017. The obtained data allows assuming the important role of the IL6 and IL10 genes which are responsible for functioning of inflammation system, in the accelerated formation of failures at the patients who had a coronary syndrome. 相似文献
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K. A. Blagodatskikh M. A. Evdokimova Yu. V. Agapkina A. G. Nikitin A. N. Brovkin A. A. Pushkov E. G. Blagodatskikh O. Yu. Kudryashova V. S. Osmolovskaya L. O. Minushkina M. S. Kochkina N. D. Selezneva E. N. Dankovtseva O. S. Chumakova T. N. Baklanova P. A. Talyzin N. E. Reznichenko O. P. Donetskaya S. N. Tereshchenko E. S. Krasil’nikova N. A. Dzhaiani E. V. Akatova M. G. Glezer A. S. Galyavich V. B. Zakirova N. A. Koziolova I. V. Timofeeva A. V. Yagoda O. I. Boeva L. I. Katel’nitskaya E. V. Khorolets S. V. Shlyk E. G. Volkova M. P. Margaryan I. O. Guz’ V. O. Konstantinov N. V. Timofeeva B. A. Sidorenko D. A. Zateishchikov V. V. Nosikov 《Molecular Biology》2010,44(5):741-747
Association between the rates of poor outcomes in the patient cohort with acute coronary syndrome and polymorphisms G(?174)C in the IL6 gene and G(?1082)A in the IL10 gene were determined. In total, 1145 patients hospitalized for coronary artery disease to cardiological hospitals of Moscow, St. Petersburg, Kazan, Chelyabinsk, Perm, Stavropol, and Rostov-on-Don were examined. The mean observation period was 9.10 ± 5.03 months (maximal, 18 months). Analysis of the survival of the patients with acute coronary syndrome that carried allele A has demonstrated that the presence of IL10 gene polymorphism G(?1082)A is associated with more frequent poor outcomes as compared with GG genotype. The survival time to endpoint for the carriers of GA and AA genotypes was 11.68 ± 0.67 months versus 12.69 ± 0.65 months for the carriers of GG genotype in IL10 gene (χ2 = 4.13, p = 0.042). As for the IL6 gene polymorphism G(?174)C, survival rate analysis did not detect any significant association with the risk for poor outcome. However, joint analysis of these polymorphisms in both genes has demonstrated that characteristic of the patients with acute coronary syndrome that carry GG genotype of IL6 gene and GA and AA genotypes of IL10 is a higher rate of poor outcomes (time to endpoint, 11.01 ± 1.24 months) as compared with the carriers of IL6 gene CC and CG genotypes and IL10 gene GG genotype (time to endpoint, 13.28 ± 0.83 months (ξ2 = 10.23, p = 0.017). These data suggest that the genes IL6 and IL10, whose products are involved in the control of inflammatory response, play an important role by increasing the probability of poor outcomes in the patients with acute coronary syndrome. 相似文献