CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR-377 to induce E2F3 expression

CircPRTM5 is associated with cell proliferation and migration in many kinds of malignancies. However, the functions and mechanisms of CircPRTM5 in CRC progression remain unclear. We explored the role and the mechanisms of CircPRTM5 in the development of CRC. Tissues of CRC patients and matched adjacent non-tumour tissues were collected to evaluate the expression of CircPRTM5. The expression of CircPRTM5 in CRC tissues was significantly higher than that in adjacent tissues. The biological functions of CircPRTM5 in CRC were determined by overexpression and down-regulation of CircPRTM5 in CRC cells in vitro and in vivo. The results indicate that knockdown of CircPRTM5 can significantly inhibit the proliferation of CRC cells. The potential mechanisms of CircPRTM5 in CRC development were identified by RT-qPCR, Western blotting analysis and luciferase reporter assay. CircPRTM5 competitively regulates the expression of E2F3 by capillary adsorption of miR-377. CircPRMT5 regulates CRC proliferation by regulating the expression of E2F3, which affects the expression of the cell cycle-associated proteins cyclinD1 and CDK2. CircPRTM5 exerts critical regulatory role in CRC progression by sponging miR-377 to induce E2F3 expression.     

Impacts of short-term nitrogen addition on the thallus nitrogen and phosphorus balance of the dominant epiphytic lichens in the Shennongjia mountains,China

Aims To better understand whether and how nitrogen addition impacts the epiphytic lichens in the Shennongjia Nature Reserve (China).     

The Biodegradation of Zein <Emphasis Type="Italic">In Vitro</Emphasis> and <Emphasis Type="Italic">In Vivo</Emphasis> and its Application in Implants

A unique polymer-based sustained-release implant drug delivery system was prepared by using biocompatible and biodegradable Zein as the skeleton meterial. After preparing Zein colloids, the Zein-loaded implant rods were formulated by injection molding followed by evaporating the solvent, and being coated with poly(lactic-co-glycolic) acid (PLGA) solution. Drug release kinetics was examined by using Fluorouracil (5-FU) as model drug. Nearly zero-order release was achieved for the model drugs for a period of 0–25 days when the implants were incubated in distilled water at 37°C. And then the degradation kinetics of the rods in vivo and in vitro were evaluated, which indicated that Zein could be absorbed by body and has good degradation property. The effects of different ratios of Zein/5-FU and the rods’ diameter on drug release were studied, respectively. The plasma concentration of 5-FU in the implants were determined by HPLC after implanting a single dose of the implants in rats. All data were subsequently processed by using the computer program 3P97, and the values were showed as follows: the area under the plasma concentration–time curve (AUC) value was 321.88 (μg/ml) × day, and the mean residence time (MRT) value was 23.05 days. The sustained-release implants of Zein/5-FU were successfully formulated. The uniqueness of the article is that Zein has been used as a skeleton material in implant delivery system for the first time and zero-order release kinetics has been obtained successfully.     

Characterization and identification of virulent Klebsiella oxytoca isolated from abalone (Haliotis diversicolor supertexta) postlarvae with mass mortality in Fujian, China

An epidemic of mass mortality of abalone (Haliotis diversicolor supertexta) postlarvae aged 40 days or less has existed across south coast of China since the second half of 2002. Among 20 bacterial strains isolated from diseased abalone postlarvae on 2216E marine agar plates during an outbreak of postlarval disease in August 2005, a predominant strain (designated strain 20) was demonstrated to be virulent to postlarvae with an LD(50) value of 1.0x10(5) colony forming units (CFUml(-1)) on day 4, while the other 19 strains were either avirulent (16 strains) or weakly virulent (3 strains). The same bacterium could be re-isolated from postlarvae after bacterial challenge using 2216E marine agar plates. Preliminary toxicity tests of ECPs of strain 20 revealed that at 2.77mgproteinml(-1), crude ECPs completely liquefied postlarvae within 24h, leaving only shells. API 20E analysis identified strain 20 as Klebsiella oxytoca. 16S and ITS rDNA sequencing and phylogenetic analyses further confirmed this identification. Antibiotic susceptibility tests showed that strain 20 exhibited 94% of susceptibility to 16 various antibiotics tested and only showed resistance to streptomycin. Results of this work demonstrated that K. oxytoca is also linked to this epidemic in Fujian, China. This is considered to be the first report regarding K. oxytoca involved in the mass mortality of postlarval abalone in south China and the world.     

The role of tamarisk in the spatial heterogeneity of soil resources in the northern Tarim Basin,Xinjiang, China

Plant and Soil - Noccaea caerulescens is a model plant for understanding metal hyperaccumulation as well as being a potential source of cultivars for phytoextraction. Sixty populations from...     

Endoscopic Biopsy in Gastrointestinal Neuroendocrine Neoplasms: A Retrospective Study

Background

Gastrointestinal neuroendocrine neoplasms (GI-NENs) are often located in the deep mucosa or submucosa, and the efficacy of endoscopic biopsy for diagnosis and treatment of GI-NENs is not fully understood.

Objective

The current study analyzed GI-NENs, especially those diagnosed pathologically and resected endoscopically, and focused on the biopsy and cold biopsy forceps polypectomy (CBP) to analyze their roles in diagnosing and treating GI-NENs.

Methods

Clinical data of all GI-NENs were reviewed from January 2006 to March 2012. Histopathology was used to diagnose GI-NENs, which were confirmed by immunohistochemistry.

Results

67.96% GI-NENs were diagnosed pathologically by endoscopy. Only 26.21% were diagnosed pathologically by biopsies before treatment. The diagnostic rate was significantly higher in polypoid (76.47%) and submucosal lesions (68.75%), than in ulcerative lesions (12.00%). However, biopsies were only taken in 56.31% patients, including 51.52% of polypoid lesions, 35.56% of submucosal lesions and 100.00% of ulcerative lesions. Endoscopic resection removed 61.76% of GI-NENs, including six by CBP, 14 by snare polypectomy with electrocauterization, 28 by endoscopic mucosal resection (EMR) and 15 by endoscopic submucosal dissection (ESD). 51.52% polypoid GI-NENs had infiltrated the submucosa under microscopic examination. CBP had a significantly higher rate of remnant (33.33%) than snare polypectomy with electrocauterization, EMR and ESD (all 0.00%).

Conclusions

Biopsies for all polypoid and submucosal lesions will improve pre-operative diagnosis. The high rate of submucosal infiltration of polypoid GI-NENs determined that CBP was inadequate in the treatment of GI-NENs. Diminutive polypoid GI-NENs that disappeared after CBP had a high risk of remnant and should be closely followed up over the long term.     

辣椒拟种群对模拟昆虫捕食行为的反应

基于拟种群理论,运用方差分析法、主分量分析法及植物生长分析法,研究7月高温期辣椒拟种群不同程度的剪叶对模拟短期虫害的反应.结果表明,辣椒具有较强的补偿能力;老叶的数量、干重和叶面积随剪叶程度的增加而下降,新叶则相反,但总叶数量相近;总叶面积和干重受老叶影响大,差异显著;枝的数量、干重、长度差异不显著;果、花、花蕾的数量差异也不显著,但干重差异显著;用数量指标代替干重、叶面积和长度等指标来进行植物的生长分析,仅对组元个体间干重极差小的拟种群适用;高温期一定程度的虫害对辣椒生长和果实产量的提高有益.     

鄂东南低山丘陵区枫香种群的天然更新方式与特征

鄂东南低山丘陵区是一个生态脆弱区, 枫香(Liquidamb ar formasana)林是该地区的重要森林植被类型, 对于维持该地区的生态安全和经济发展具有重要意义。为了探明枫香实生和萌芽更新对枫香种群维持的作用, 采用样方法研究了该地区枫香林和马尾松(Pinus massoniana)-枫香混交林枫香种群的天然更新方式, 并采用不同年代伐桩构建的时间序列研究了枫香伐桩的萌芽更新规律。结果表明: (1) 该地区的枫香种群由实生和萌芽更新植株组成, 但二者的比例在马尾松-枫香混交林和枫香林间存在明显差异; (2) 萌芽更新可以划分为幼苗萌芽和伐桩萌芽更新2类, 其中幼苗萌芽更新是对自然环境的适应, 伐桩萌芽更新是对人类砍伐的适应; (3) 枫香伐桩萌芽更新存在明显的自疏现象, 并最终形成1-2杆的萌代主; 与实生幼苗相比,伐桩萌条具有生长迅速的特点。上述结果表明, 枫香的实生和萌芽更新共同维持了该地区枫香种群的稳定。     

高原鼢鼠和甘肃鼢鼠胆汁无机元素含量分析

<正>无机元素在动物发育、免疫调节、代谢和繁殖等方面发挥着重要作用(刘季科等,1991;蒲俊华等,2010;Talebnejadetal.,2021;Gashkinaet al., 2022)。现代医学已证明,无机元素的种类和含量影响药材的药性和药效(谭文敏,2015;陈虹等,2017),其已逐渐成为动物药鉴别、炮制加工、质量评价和寻找替代品的重要依据(罗兴洪等,2004)。熊胆属于传统珍稀濒危动物药,