全文获取类型
收费全文 | 6632篇 |
免费 | 654篇 |
国内免费 | 2篇 |
出版年
2024年 | 4篇 |
2023年 | 39篇 |
2022年 | 53篇 |
2021年 | 126篇 |
2020年 | 91篇 |
2019年 | 114篇 |
2018年 | 167篇 |
2017年 | 128篇 |
2016年 | 229篇 |
2015年 | 401篇 |
2014年 | 417篇 |
2013年 | 487篇 |
2012年 | 632篇 |
2011年 | 576篇 |
2010年 | 370篇 |
2009年 | 312篇 |
2008年 | 403篇 |
2007年 | 396篇 |
2006年 | 353篇 |
2005年 | 315篇 |
2004年 | 353篇 |
2003年 | 302篇 |
2002年 | 264篇 |
2001年 | 57篇 |
2000年 | 46篇 |
1999年 | 66篇 |
1998年 | 90篇 |
1997年 | 48篇 |
1996年 | 31篇 |
1995年 | 47篇 |
1994年 | 36篇 |
1993年 | 32篇 |
1992年 | 35篇 |
1991年 | 23篇 |
1990年 | 25篇 |
1989年 | 23篇 |
1988年 | 22篇 |
1987年 | 11篇 |
1986年 | 10篇 |
1985年 | 16篇 |
1984年 | 12篇 |
1983年 | 15篇 |
1982年 | 11篇 |
1981年 | 14篇 |
1980年 | 13篇 |
1979年 | 9篇 |
1978年 | 14篇 |
1977年 | 6篇 |
1976年 | 9篇 |
1974年 | 9篇 |
排序方式: 共有7288条查询结果,搜索用时 15 毫秒
1.
Response of fourth-instar larvae of Anopheles albimanus Wiedemann (Diptera: Culicidae) to food and inert particles floating at the water surface was studied. In a choice test, larvae aggregated at powdered organic materials (blood meal, liver powder alfalfa flour and wheat flour) but not at inert materials (kaolin, chalk or charcoal). Larvae responded positively to proteins as well as some carbohydrates, but not to cellulose. Retention of larvae at food sources found by random locomotion was found to be responsible for larval aggregation. Larvae ingested food particles 6–9 times faster than insert particles. The significance of Anopheline feeding behavior in the development of formulations of stomach toxins (bacterial agents) used in larval control is discussed.
Zusammenfassung Die vorliegende Studie befasst sich mit Verhaltensreaktionen von Anopheles albimanus Viertlarven auf an der Wasseroberfläche schwimmende Partikel. Verteilung und Orientierung der Larven wurde in einer Wahlapparatur quantifiziert. Nach Auftrag von Alfalfamehl, Weizenmehl, Stärke, Blutmehl, Leberpulver und Fischmehl wurde Aggregation der Larven in den beköderten Fächern der Apparatur beobachtet. Sowohl Proteine (Casein) als auch einige Kohlehydrate (Amylose, Amylopectin) lösten Aggregationen der Larven aus. Im Unterschied dazu führte Auftrag von Kreide, Kaolin, Polyaethylenpulver, Talcum oder Cellulose nicht zu Aggregationen. Zur Beschreibung der Entstehung larvaler Aggregationen bei Futterstoffen wurden die Schwimmbewegungen der Larven in Anwesenheit von Weizenmehl als Ködersubstanz quantifiziert. Da keine Attraktion der Larven im Sinne einer gerichteten Schwimmbewegung beobachtet werden konnte, wird geschlussfolgert, dass sofortige Beendung der Suchaktivität der Larven bei zufällig gefundenen Futterquellen für die beobachteten Aggregationen bei organischen Substanzen verantwortlich ist.Die Fressraten der Larven bei Angebot verschiedener Substanzen im Überschuss wurde bestimmt. Larven fülten drei von insgesamt sechs Darmabschnitten innerhalb von 15–30 min bei Angebot von Futtersubstanzen, während die Füllung von nur zwei Darmabschnitten mit inerten Materialien erst nach 90–120 min zu beobachten war. Die Resultate werden in Bezug auf wasseroberflächengebundene Formulierungen von Frassgiften diskutiert. Inerte Trägersubstanzen werden wahrscheinlich wesentlich langsamer aufgenommen als Futtersubstanzen. Da An. albimanus Larven nicht von Futterquellen angezogen werden, ist eine rasche und wirksame Giftaufnahme besonders dann zu erwarten, wenn die gesamte Oberfläche der Brutgewässer mit toxinhaltigen Trägerpartikeln bedeckt werden kann.相似文献
2.
3.
Christoph D. Dahl Nikos K. Logothetis Heinrich H. Bülthoff Christian Wallraven 《Proceedings. Biological sciences / The Royal Society》2010,277(1696):2973-2981
Primates possess the remarkable ability to differentiate faces of group members and to extract relevant information about the individual directly from the face. Recognition of conspecific faces is achieved by means of holistic processing, i.e. the processing of the face as an unparsed, perceptual whole, rather than as the collection of independent features (part-based processing). The most striking example of holistic processing is the Thatcher illusion. Local changes in facial features are hardly noticeable when the whole face is inverted (rotated 180°), but strikingly grotesque when the face is upright. This effect can be explained by a lack of processing capabilities for locally rotated facial features when the face is turned upside down. Recently, a Thatcher illusion was described in the macaque monkey analogous to that known from human investigations. Using a habituation paradigm combined with eye tracking, we address the critical follow-up questions raised in the aforementioned study to show the Thatcher illusion as a function of the observer''s species (humans and macaques), the stimulus'' species (humans and macaques) and the level of perceptual expertise (novice, expert). 相似文献
4.
The cytomegalovirus enhancer: a pan-active control element in transgenic mice. 总被引:41,自引:2,他引:39 下载免费PDF全文
In an effort to identify widely active positive regulatory elements, we have examined the action of the cytomegalovirus enhancer-promoter in transgenic mice. These elements activated expression in 24 of 28 tissues tested. The greatest expression was observed in the heart, kidney, brain, and testis. Maximum expression further localized to specific cells within the heart and kidney. 相似文献
5.
Alexander Wiedenmann Peter Dimroth Christoph von Ballmoos 《Molecular microbiology》2009,72(2):479-490
F1 F0 ATP synthases use the electrochemical potential of H+ or Na+ across biological membranes to synthesize ATP by a rotary mechanism. In bacteria, the enzymes can act in reverse as ATP-driven ion pumps creating the indispensable membrane potential. Here, we demonstrate that the F0 parts of a Na+ - and H+ -dependent enzyme display major asymmetries with respect to their mode of operation, reflected by the requirement of ∼100 times higher Na+ or H+ concentrations for the synthesis compared with the hydrolysis of ATP. A similar asymmetry is observed during ion transport through isolated F0 parts, indicating different affinities for the binding sites in the a/c interface. Together with further data, we propose a model that provides a rationale for a differential usage of membrane potential and ion gradient during ATP synthesis as observed experimentally. The functional asymmetry might also reflect an important property of the ATP synthesis mechanism in vivo . In Escherichia coli , we observed respiratory chain-driven ATP production at pH 7–8, while P -site pH values < 6.5 were required for ATP synthesis in vitro . This discrepancy is discussed with respect to the hypothesis that during respiration lateral proton diffusion could lead to significant acidification at the membrane surface. 相似文献
6.
7.
8.
Mirjam Frischknecht Vidhya Jagannathan Philippe Plattet Markus Neuditschko Heidi Signer-Hasler Iris Bachmann Alicja Pacholewska Cord Dr?gemüller Elisabeth Dietschi Christine Flury Stefan Rieder Tosso Leeb 《PloS one》2015,10(10)
The identification of quantitative trait loci (QTL) such as height and their underlying causative variants is still challenging and often requires large sample sizes. In humans hundreds of loci with small effects control the heritable portion of height variability. In domestic animals, typically only a few loci with comparatively large effects explain a major fraction of the heritability. We investigated height at withers in Shetland ponies and mapped a QTL to ECA 6 by genome-wide association (GWAS) using a small cohort of only 48 animals and the Illumina equine SNP70 BeadChip. Fine-mapping revealed a shared haplotype block of 793 kb in small Shetland ponies. The HMGA2 gene, known to be associated with height in horses and many other species, was located in the associated haplotype. After closing a gap in the equine reference genome we identified a non-synonymous variant in the first exon of HMGA2 in small Shetland ponies. The variant was predicted to affect the functionally important first AT-hook DNA binding domain of the HMGA2 protein (c.83G>A; p.G28E). We assessed the functional impact and found impaired DNA binding of a peptide with the mutant sequence in an electrophoretic mobility shift assay. This suggests that the HMGA2 variant also affects DNA binding in vivo and thus leads to reduced growth and a smaller stature in Shetland ponies. The identified HMGA2 variant also segregates in several other pony breeds but was not found in regular-sized horse breeds. We therefore conclude that we identified a quantitative trait nucleotide for height in horses. 相似文献
9.
Norbert Rieder 《Hydrobiologia》1987,145(1):175-181
A group of nine sensory setae is found on the tip of the antennula ofDaphnia magna in both sexes. Inside a seta four dendrites are situated, each with one receptor cilium. The receptor cilia extend through
a liquor space into the exterior part of the seta.
The exterior part of the liquor space is divided from the interior part by a knob-like thickening of the innermost layer of
the epicuticle, the basal bead. The basal bead narrows the liquor space and the receptor cilia. The interior part of the liquor
space is surrounded by five sheath cells, the exterior part by a thin cuticle. In the exterior part the receptor cilia branch
partly and reach a terminal pellet on the tip of the seta. The terminal pellet is a thickened part of the epicuticle. It is
permeable to several dissolved substances.
It is the exterior part of the receptor that projects over the tip of the antennula and seems to be the entire seta. During
the premoult the fifth sheath cell builds up the articulation of the seta, the fourth the basal bead, and the third the shaft
of the seta. The first sheath cell forms the cuticular sheath.
The organ seems to be a chemoreceptor, but the adequate stimulus is as yet unknown. 相似文献
10.