全文获取类型
收费全文 | 921篇 |
免费 | 81篇 |
出版年
2023年 | 3篇 |
2022年 | 10篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 12篇 |
2018年 | 9篇 |
2017年 | 10篇 |
2016年 | 21篇 |
2015年 | 36篇 |
2014年 | 40篇 |
2013年 | 63篇 |
2012年 | 75篇 |
2011年 | 79篇 |
2010年 | 52篇 |
2009年 | 42篇 |
2008年 | 46篇 |
2007年 | 64篇 |
2006年 | 61篇 |
2005年 | 58篇 |
2004年 | 42篇 |
2003年 | 39篇 |
2002年 | 47篇 |
2001年 | 8篇 |
2000年 | 10篇 |
1999年 | 10篇 |
1998年 | 10篇 |
1997年 | 11篇 |
1996年 | 7篇 |
1995年 | 13篇 |
1994年 | 13篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1990年 | 7篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1975年 | 4篇 |
1971年 | 4篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1966年 | 5篇 |
1965年 | 3篇 |
排序方式: 共有1002条查询结果,搜索用时 296 毫秒
1.
2.
Reductive dechlorination of 1,2-dichloroethane and chloroethane by cell suspensions of methanogenic bacteria 总被引:4,自引:0,他引:4
Christof Holliger Gosse Schraa Alfons J. M. Stams Alexander J. B. Zehnder 《Biodegradation》1990,1(4):253-261
Concentrated cell suspensions of methanogenic bacteria reductively dechlorinated 1,2-dichloroethane via two reaction-mechanisms: a dihalo-elimination yielding ethylene and two hydrogenolysis reactions yielding chloroethane and ethane, consecutively. The transformation of chloroethane to ethane was inhibited by 1,2-dichloroethane. Stimulation of methanogenesis caused an increase in the amount of dechlorination products formed, whereas the opposite was found when methane formation was inhibited. Cells of Methanosarcina barkeri grown on H2/CO2 converted 1,2-dichloroethane and chloroethane at higher rates than acetate or methanol grown cells.Abbreviations BrES
2-bromoethanesulfonic acid
- CA
chloroethane
- 1,2-DCA
1,2-dichloroethane
- F430
Ni(II)tetrahydro-(12, 13)-corphin with an uroporphinoid (III) ligand skeleton 相似文献
3.
J. B. Warchol Ch. Pilgrim W. J. Ginda R. Brelińska 《Histochemistry and cell biology》1988,88(3-6):571-573
Summary Wistar rats were injected intraperitoneally with 2-(14C)deoxyglucose and their thymuses were processed for thaw-mount autoradiography after 5, 10 or 35 min. Highest levels of radioactivity were demonstrated in the thymic medulla (5-fold higher than in the cortex). Scanning of autoradiograms for regional differences in grain densities indicated particularly intense glucose utilization in the cortico-medullary zone. Differences in glucose utilization between individual thymic zones seem to reflect differences in cellular composition, i. e., ratio of stroma cells to thymocytes.Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday 相似文献
4.
OBJECTIVE--To determine whether a set of physical symptoms is associated with low blood pressure and to investigate the possible role of psychological factors in their occurrence. DESIGN--Analysis of data collected by questionnaire and physical screening from the first phase of the Whitehall II study, a cohort study of an employed population. SETTING--23 civil service departments in London. SUBJECTS--10,314 male and female London based civil servants aged between 35 and 55. MAIN OUTCOME MEASURES--Symptoms of dizziness-giddiness and unexplained tiredness; psychological functioning as measured by the 30 item general health questionnaire in which the response "no more than usual" to an item about disease was scored as indicating chronic illness. RESULTS--Dizziness-giddiness in men and unexplained tiredness in both men and women were significantly related to low systolic blood pressure. There was a highly significant inverse relation between general health questionnaire score and systolic blood pressure for both men and women, which persisted after controlling for potentially confounding variables, including age, body mass index, drug treatment, physical illness, and exercise. This association of low blood pressure with physical symptoms was no longer significant when general health questionnaire score was controlled for. CONCLUSIONS--There seems a strong relation between low systolic blood pressure and minor psychological dysfunction. Associated physical symptoms seem to be secondary to the primary disturbance in mental state. 相似文献
5.
Philippe Szankasi Christof Gysler Ulrich Zehntner Urs Leupold Jürg Kohli Peter Munz 《Molecular & general genetics : MGG》1986,202(3):394-402
Summary Recombination between dispersed yet related serine tRNA genes of Schizosaccharomyces pombe does occur during mitosis but it is approximately three orders of magnitude less frequent than in meiosis. Two mitotic events have been studied in detail. In the first, a sequence of at least 18 nucleotides has been transferred from the donor sup3 gene on the right arm of chromosome I to the related acceptor gene sup12 on the left arm of the same chromosome, thereby leading to the simultaneous change of 8 bp in the acceptor gene. This event must be explained in terms of recombination rather than mutation. It is assumed that it represents mitotic gene conversion, although it was not possible to demonstrate that the donor gene had emerged unchanged from the event. The second case reflects an interaction between sup9 on chromosome III and sup3 on chromosome I. Genetic and physical analysis allows this event to be described as mitotic gene conversion associated with crossingover. The result of this event is a reciprocal translocation. No further chromosomal aberrations were found among an additional 700 potential intergenic convertants tested. Thus intergenic conversion is much less frequently associated with crossingover than allelic conversion. However, the rare intergenic conversion events associated with crossingover provide a molecular mechanism for chromosomal rearrangements. 相似文献
6.
7.
Cells of a mutant in vivo subline of the Ehrlich-Lettré mouse ascites tumour (ELAT) were converted to growth in suspension culture. Kinetic analysis revealed the selective character of the conversion process; without a detectable adaptation period, a fraction of about 2 × 10-5 of the explanted cells continued to grow in vitro. the resulting, mutant Ehrlich-Lettré ascites cell strain was designated HD33 and propagated uninterruptedly from 1974 on. the corresponding in vivo ELAT subline HD33 was derived from the HD33 ascites cell strain by intraperitoneal retransplantation. In HD33 cell suspension cultures, the population doubling time, the average intermitotic interval, as determined by videomonitoring, and the average duration of the cell cycle, as determined from percentage of labelled mitoses (PLM) data, were all measured at 15 hr. Cell loss and quiescent compartments were insignificant. the duration of the G1 phase was effectively zero. Both PLM data and [3H]/[14C] thymidine double-labetling measurements revealed an S-phase duration of between 11 and 12 hr. the G2 phase lasted 3–5 hr. The HD33 strain differs from comparable suspension strains of wild-type Ehrlich ascites cells in the insignificant role of density-dependent inhibition in growth, and the striking prolongation of the S phase which is associated with an excessive, cytoplasmic storage of glycogen by the mutant cells. 相似文献
8.
Bound atrazine was detected inElodea canadensis by an improved immunohistochemical fluorescence procedure using anti-triazine antibodies from rabbits, biotin-labelled anti-rabbit
immunoglobulin G and streptavidin-phycoerythrin conjugate. Whereas no labelling was found in control plants grown in charcoal-filtered,
atrazine-free water, the labelling of plants obtained from their natural habitat and grown in tap water was sometimes nearly
as high as in samples loaded with atrazine. The efficiency of the immunofluorescence procedure was compared using several
antisera obtained by immunizing with different hapten conjugates and purified by various purification methods. The best results
were observed with the atrazine analogue ametryn sulfoxide, which was coupled to bovine serum albumin for immunization and
to Sepharose for immunoaffinity chromatography. The procedure described in this paper may serve as a general tool for detecting
bound pesticide residues in plant material.
Dedicated to Professor Hans Mohr on the occasion of his 60th birthday 相似文献
9.
Christof Brunner Hans Lassmann Thomas V. Waehneldt Jean-Marie Matthieu† Christopher Linington‡ 《Journal of neurochemistry》1989,52(1):296-304
In a light and electron microscopic immunocytochemical study we have examined the distribution of myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), and myelin/oligodendroglial glycoprotein (MOG) within CNS myelin sheaths and oligodendrocytes of adult Sprague-Dawley rats. Ultrastructural immunocytochemistry allowed quantitative analysis of antigen density in different myelin and oligodendrocyte zones: MBP was detectable in high density over the whole myelin sheath, but not in regions of loops, somata, or the oligodendrocyte plasma membrane. CNP reactivity was highest at the myelin/axon interface, and found in lower concentration over the outer lamellae of myelin sheaths, at the cytoplasmic face of oligodendrocyte membranes, and throughout the compact myelin. MOG was preferentially detected at the extracellular surface of myelin sheaths and oligodendrocytes and in only low amounts in the lamellae of compacted myelin and the myelin/axon border zone. Our studies, thus, indicate further the presence of different molecular domains in compact myelin, which may be functionally relevant for the integrity and maintenance of the myelin sheath. 相似文献
10.
B A Bernard J M Bernardon C Delescluse B Martin M C Lenoir J Maignan B Charpentier W R Pilgrim U Reichert B Shroot 《Biochemical and biophysical research communications》1992,186(2):977-983
The action of retinoids on gene regulation is mediated by three distinct nuclear retinoic acid receptor (RAR) subtypes called RAR alpha, beta and gamma. Since RAR gamma is predominantly expressed in adult skin, specific ligands for this subtype could (i) represent valuable tools to evaluate the biological role of RAR gamma in skin and (ii) provide therapeutic entities with a higher therapeutic index at lower teratogenic risk. Using in vitro binding studies and a functional transactivation assay, we have identified three compounds with high RAR gamma selectivity. 相似文献