排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
Kristen L. Choffe Jerrin M. R. Victor Susan J. Murch Praveen K. Saxena 《In vitro cellular & developmental biology. Plant》2000,36(1):30-36
Summary An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation
Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole
explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ)
in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants
with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established
a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate
mass production of high-quality E. purpurea plants for the commercial market. 相似文献
2.
Gene expression during Ustilago maydis diploid filamentous growth: EST library creation and analyses
Ustilago maydis is an important model system for the plant pathogenic smut and rust fungi. Critical to the continued development of this model is establishing genomic resources. We have constructed a cDNA library from a forced diploid culture of U. maydis growing as filaments and have generated 7455 ESTs that are assembled into 3074 contiguous sequences. This represents as much as 46% of the coding capacity predicted for U. maydis. BLAST searches with a similarity cutoff of E = 10(-5), allow us to annotate 59% of the contigs based upon matches in the NCBI nr and dbEST databases. These annotated sequences provide information on mature mRNAs that will aid with gene prediction in the U. maydis genome sequence. Functional categorization and comparative analyses of the sequences provides gene identities, expression information and a solid base for future research in this model fungal pathogen. 相似文献
3.
S. J. Murch K. L. Choffe J. M. R. Victor T. Y. Slimmon S. KrishnaRaj P. K. Saxena 《Plant cell reports》2000,19(6):576-581
St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development
of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N′-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hypocotyl segments of St. John's wort seedlings. The optimum level of thidiazuron supplementation to
the culture medium was 5 μmol·l–1 for a 9-day induction period followed by subculture of induced hypocotyl explants on basal medium. Other plant growth regulators
including benzyladenine and indoleacetic acid were not effective in inducing regeneration on St. John's wort hypocotyls. Histological
examination of the cultures revealed that the regenerated plants were derived from de novo developed shoots. Transfer of the regenerated shoots into a liquid medium with no plant growth regulators resulted in the
rapid and prolific growth of viable plantlets. The rapid and efficient micropropagation system for St. John's wort may be
useful for both the genetic improvement of this crop and the production of high-quality phytopharmaceutical preparations for
the treatment of neurological disorders.
Received: 19 March 1999 / Revision received: 5 July 1999 · Accepted: 17 August 1999 相似文献
4.
Choffe Kristen L. Murch Susan J. Saxena Praveen K. 《Plant Cell, Tissue and Organ Culture》2000,62(3):227-234
An in vitro propagation system was developed for Echinacea purpureaL. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Root organogenesis
from Echinacea purpurea hypocotyl explants was effectively induced by indolebutyric acid. Indoleacetic acid was found to be less effective than indolebutyric
acid while treatments with naphthaleneacetic acid were ineffective for induction of root organogenesis. The results of this
study have established a micropropagation system for Echinacea purpurea that will provide axenic plant material for further investigations into medicinally active biochemicals and the mass production
of high-quality Echinacea purpurea root tissues for the commercial market.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
1