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1.
Gus'kova OA Khalatur PG Khokhlov AR Chinarev AA Tsygankova SV Bovin NV 《Bioorganicheskaia khimiia》2010,36(5):622-629
The full-atomic molecular dynamics (MD) simulation of adsorption mode for diantennary oligoglycines [H-Gly4-NH(CH2)5]2 onto graphite and mica surface is described. The resulting structure of adsorption layers is analyzed. The peptide second structure motives have been studied by both STRIDE (structural identification) and DSSP (dictionary of secondary structure of proteins) methods. The obtained results confirm the possibility of polyglycine II (PGII) structure formation in diantennary oligoglycine (DAOG) monolayers deposited onto graphite surface, which was earlier estimated based on atomic-force microscopy measurements. 相似文献
2.
I. V. Gorokhova A. A. Chinarev A. B. Tuzikov S. V. Tsygankova N. V. Bovin 《Russian Journal of Bioorganic Chemistry》2006,32(5):420-428
Linear oligoglycines of various lengths bearing a carboxyl or an amide group at their C-termini and also their poly(acrylamide) conjugates were synthesized. No self-assembly into supramolecular structures was observed for free oligoglycines H-(Gly)m-OH (m = 3–5). At the same time, oligoglycylamides H-(Gly)m-NH2 (m = 3–5) demonstrated ability for both self-assembly in aqueous solution and assembly promoted by an additional interaction with surface. In the case of polymer-bound oligoglycines (and their amides), no intramolecular clustering of peptide chains, as expected, was observed. This means that the presence of several oligoglycine chains bound to each other in one center is not a necessary prerequisite for polyglycine II-type association. 相似文献
3.
Tsvetkov DE Cheshev PE Tuzikov AB Chinarev AA Pazynina GV Sablina MA Gambarian AS Bovin NV Rieben R Shashkov AS 《Bioorganicheskaia khimiia》2002,28(6):518-534
Neoglycoconjugates containing 4, 8, 16, 32, and 64 terminal residues of B-disaccharide (BDI) or N-acetylneuraminic acid (Neu5Ac) attached to poly(aminoamide)-type dendrimers (PAMAMs) were synthesized. The ability of BDI conjugates to bind natural xenoantibodies (anti-BDI antibodies) and the ability of Neu5Ac conjugates to inhibit the hemagglutinin-mediated adhesion of influenza virus were studied. The biological activity of PAMAM conjugates turned out to be higher than that of free carbohydrate ligands, but less than that of multivalent glycoconjugates based on other types of synthetic polymeric carriers. A conformational analysis of PAMAM matrices and resulting conjugates was performed to determine the statistical distances between carbohydrate ligands. The computations revealed the tendency of the PAMAM chains toward compaction and formation of dense globules. The process results in a decrease in the distances between the carbohydrate ligands in the conjugates and, hence, could affect the ability of glycoconjugates to efficiently bind the polyvalent carbohydrate-recognizing proteins. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2002, vol. 28, no. 6; see also http://www.maik.ru. 相似文献
4.
Shilova NV Galanina OE Pochechueva TV Chinarev AA Kadykov VA Tuzikov AB Bovin NV 《Glycoconjugate journal》2005,22(1-2):43-51
Adsorption of a carbohydrate on solid phase is the necessary stage of the immunosorbent assay (ELISA) and analogous methods of the study of carbohydrate-protein interaction. Usually physical adsorption on polystyrene requires a high concentration of conjugated carbohydrate and, thus, enormous consumption of it. In this study, we explored two approaches allowing more rational use of oligosaccharide (Glyc). The first of them is based on the covalent immobilization of neoglycoconjugates on the NH2-modified polystyrene; the second one is based on the elevated adherence of high m.w. neoglycoconjugates to polystyrene. Covalent immobilization of polyacrylamide conjugates, Glyc-PAA, provided a possibility to solve the problem, but the nonspecific binding of antibodies in ELISA proved to be unacceptably high. At the same time, the increase of the Glyc-PAA m.w. from 30 kDa to 2,000 kDa allowed a 10–20 fold decrease of its consumption, when using physical adsorption, whereas the assay background remained at the low level. The amount of 2,000 kDa Glyc-PAA that is sufficient for the coating of a standard 96-well plate corresponds to the nanomole level of oligosaccharide, this providing a possibility to use saccharides that are available in a very limited amount when studying the carbohydrate-protein interaction with solid-phase techniques. Published in 2005. 相似文献
5.
Pochechueva T Jacob F Goldstein DR Huflejt ME Chinarev A Caduff R Fink D Hacker N Bovin NV Heinzelmann-Schwarz V 《Glycoconjugate journal》2011,28(8-9):507-517
Anti-glycan antibodies represent a vast and yet insufficiently investigated subpopulation of naturally occurring and adaptive antibodies in humans. Recently, a variety of glycan-based microarrays emerged, allowing high-throughput profiling of a large repertoire of antibodies. As there are no direct approaches for comparison and evaluation of multi-glycan assays we compared three glycan-based immunoassays, namely printed glycan array (PGA), fluorescent microsphere-based suspension array (SA) and ELISA for their efficacy and selectivity in profiling anti-glycan antibodies in a cohort of 48 patients with and without ovarian cancer. The ABO blood group glycan antigens were selected as well recognized ligands for sensitivity and specificity assessments. As another ligand we selected P(1), a member of the P blood group system recently identified by PGA as a potential ovarian cancer biomarker. All three glyco-immunoassays reflected the known ABO blood groups with high performance. In contrast, anti-P(1) antibody binding profiles displayed much lower concordance. Whilst anti-P(1) antibody levels between benign controls and ovarian cancer patients were significantly discriminated using PGA (p=0.004), we got only similar results using SA (p=0.03) but not for ELISA. Our findings demonstrate that whilst assays were largely positively correlated, each presents unique characteristic features and should be validated by an independent patient cohort rather than another array technique. The variety between methods presumably reflects the differences in glycan presentation and the antigen/antibody ratio, assay conditions and detection technique. This indicates that the glycan-antibody interaction of interest has to guide the assay selection. 相似文献
6.
O. A. Gus’kova P. G. Khalatur A. R. Khokhlov A. A. Chinarev S. V. Tsygankova N. V. Bovin 《Russian Journal of Bioorganic Chemistry》2010,36(5):574-580
An atomistic molecular dynamics (MD) simulation of the adsorption of biantennary oligoglycine [H-Gly4-NH(CH2)5]2 onto a graphite and mica surface is described. The structure of the resultant adsorption layers is analyzed. The secondary
structure motifs of peptide blocks are studied by the STRIDE (structural identification) and DSSP (dictionary of the secondary
structure of proteins) methods. The results of the study confirm the possibility of forming a polyglycine-II (PGII) structure
in the monolayers of biantennary oligoglycines (BAOG) on a graphite surface previously supposed from the data of atomic force
microscopy. 相似文献
7.
Larisa Mochalova Rick Bright Xiyan Xu Elena Korchagina Alexander Chinarev Niсolai Bovin Alexander Klimov 《Glycoconjugate journal》2010,27(3):321-327
Influenza virus neuraminidase inhibitors (NAIs), currently used as anti-influenza drugs, can lead to the appearance of drug-resistant
variants. Resistance to NAIs appears due to mutations in the active site of the neuraminidase (NA) molecule that decrease
the NA enzymatic activity and sometimes in the hemagglutinin (HA) that decrease its affinity for cell receptors and, therefore,
reduce the requirement for NA activity in releasing mature virions from infected cells. Using a set of sialo-oligosaccharides,
we evaluated changes in the receptor-binding specificity of the HA and substrate specificity of the NA of influenza B viruses
that had acquired resistance to NAIs. The oligosaccharide specificity of two pairs of field influenza B viruses, namely: i)
B/Memphis/20/96 and its NAI-resistant variant, B/Memphis/20-152K/96, containing mutation R152K in the NA and 5 amino acid
substitutions in the HA1, and ii) B/Hong Kong/45/2005 and its NAI-resistant variant B/Hong Kong/36/2005, containing a single
R371K mutation in the NA, was evaluated. Wild type viruses bound strictly to a “human type” receptor, α2-6-sialo-oligosaccharide
6`SLN, but desialylated it is approximately 8 times less efficiently than the α2-3 sialosaccharides. Both drug-resistant viruses
demonstrated the ability to bind to “avian type” receptors, α2-3 sialo-oligosaccharides (such as 3`SLN), whereas their affinity
for 6`SLN was noticeably reduced in comparison with corresponding wild type viruses. Thus, the development of the NAI resistance
in the studied influenza B viruses was accompanied by a readjustment of HA-NA oligosaccharide specificities. 相似文献
8.
The 2,2'-azodiisobutyronitrile-induced radical polymerization in solution of 4-nitrophenyl acrylate was performed under microwave heating at a frequency of 2.45 GHz. This approach allows the control of the molecular mass of poly(4-nitrophenyl acrylate) used in the synthesis of multivalent glycoconjugates. It was found that the polymerization of 4-nitrophenyl acrylate under microwave irradiation results in products with a narrower molecular mass distribution than at conventional heating. 相似文献
9.
Gorokhova IV Chinarev AA Tuzikov AB Tsygankova SV Bovin NV 《Bioorganicheskaia khimiia》2006,32(5):467-476
Linear oligoglycines of various lengths bearing a carboxyl or an amide group at their C-termini and also their poly(acrylamide) conjugates were synthesized. No self-assembly into supramolecular structures was observed for free oligoglycines H-(Gly)m-OH(m = 3-5). At the same time, oligoglycylamides H-(Gly)m-NH2 (m = 3-5) demonstrated ability for both self-assembly in aqueous solution and assembly promoted by an additional interaction with surface. In the case of polymer-bound oligoglycines (and their amides), no intramolecular clustering of peptide chains, as expected, was observed. This means that the presence of several oligoglycine chains bound to each other in one center is not a necessary prerequisite for polyglycine II-type association. 相似文献
10.
Multimeric glycotherapeutics: New paradigm 总被引:3,自引:0,他引:3
The general principle of anti-adhesion therapy is the inhibition of microorganism adhesion to the host cell with the help of a soluble receptor analog. Despite an evident attractiveness of the concept and its long existence, the therapeutics of the post-antibiotic era have not yet appeared. This can be explained by the contradictoriness of requirements for anti-adhesion drugs: to be efficient a drug must be multivalent, i.e. large molecule, but to obtain FDA approval it should be a small molecule. A way to overcome this contradiction is self-assembly of glycopeptides. The carbohydrate part of glycopeptide is responsible for binding with the lectin of microorganisms, whereas a simple peptide part is responsible for an association to the so-called tectomers. Depending on the structure, tectomers are formed either spontaneously or upon promotion of a microorganism. In particular, sialopeptide, which is capable of converting to a tectomer only in the presence of the influenza virus, has been obtained. Thus, the new strategy of anti-adhesion therapy can be formulated as follows: (1) identification of oligosaccharide-receptor for a particular virus (bacteria); (2) optimization of the peptide part; (3) conventional trials. The expected advantages of this strategy are the following: (i) no polymer; (ii) a virion completely covered with a tectomer, i.e. blocking is both complete and irreversible; (iii) rapid and rational lead identification and optimization; (iv) minimum side effects; (v) potential for microorganism resistance to natural receptor is lower than in the case of mimetics. Published in 2004. 相似文献