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排序方式: 共有686条查询结果,搜索用时 15 毫秒
1.
利用KCN、H_2O2和SDS的选择性反应引起的SOD同工酶谱带变化即可鉴别粗抽提液中的SOD同工酶类型;用这种方法对五株不同种根霉的鉴别实验表明,它们均不同程度地含有Cu,Zn型和Mn型两种SOD,前者约占80%左右,后者只占20%左右。用邻苯三酚自氧化法对样品中SOD活性测定结果与在同工酶谱上的鉴别结果基本一致。 相似文献
2.
Dianye Zhang Lu Wang Shuqi Qin Dan Kou Siyu Wang Zhihu Zheng Josep Peñuelas Yuanhe Yang 《Global Change Biology》2023,29(14):3910-3923
The status of plant and microbial nutrient limitation have profound impacts on ecosystem carbon cycle in permafrost areas, which store large amounts of carbon and experience pronounced climatic warming. Despite the long-term standing paradigm assumes that cold ecosystems primarily have nitrogen deficiency, large-scale empirical tests of microbial nutrient limitation are lacking. Here we assessed the potential microbial nutrient limitation across the Tibetan alpine permafrost region, using the combination of enzymatic and elemental stoichiometry, genes abundance and fertilization method. In contrast with the traditional view, the four independent approaches congruently detected widespread microbial nitrogen and phosphorus co-limitation in both the surface soil and deep permafrost deposits, with stronger limitation in the topsoil. Further analysis revealed that soil resources stoichiometry and microbial community composition were the two best predictors of the magnitude of microbial nutrient limitation. High ratio of available soil carbon to nutrient and low fungal/bacterial ratio corresponded to strong microbial nutrient limitation. These findings suggest that warming-induced enhancement in soil nutrient availability could stimulate microbial activity, and probably amplify soil carbon losses from permafrost areas. 相似文献
3.
在无溶剂系统中固定化脂肪酶合成聚乙二醇400月桂酸酯 总被引:4,自引:1,他引:3
在无溶荆反应系统中,研究了固定化假丝酵母(Candida sp)-1619脂肪酶催化合成聚乙二醇400(PEG400)月桂酸酯的酯化条件。在反应过程中不断脱水和使月桂酸的量高于化学计量值的方法,使酯化率明显提高。分批补加PEG400使产量进一步增加。在5.0mmol月桂酸.2.5mmolPEG400,20mg同定化脂肪酶(200u),O.2ml水组成的反应体系中,40℃,锥形瓶敞口振荡反应48h。醑化率达91%;在负压条件下反应.酯化率达98.9%;反应体系中月桂酸的董增加到6.0mmol时,PEG400完全被酯化。用己烷提取产物的收率为95%.通过薄层色谱鉴定酯化产物为双酯。 相似文献
4.
高必需氨基酸转基因马铃薯的研究 总被引:8,自引:0,他引:8
80年代以来,马铃薯遗传转化系统日趋成熟,转基因工程植株已被广泛应用于基础科学研究[1]。作为食物蛋白和能量主要来源的马铃薯,提高其蛋白质含量及质量的遗传工程研究正受到人们的普遍关注[2]。Yang等[2]将旨在改善氨基酸平衡的CAT-HEAAE(氯酶素乙酰转移酶-高含量人体必需氨基酸)融合基因导入马铃薯,获得了Southernblot、Northernblot、Westernblot的证据,但尚缺少氨基酸分析的资料。玉米醇溶蛋白(zein)[3]是一个富含甲硫氨酸的贮存蛋白,它和人工合成的HE… 相似文献
5.
Kou Kubota 《Biochemical and biophysical research communications》1982,105(2):688-697
A growing organism that produces antibiotic peptide was incubated with L-(U-14C)serine for labeling linear gramicidin. Linear gramicidin was isolated by a simple chromatographic method from tyrothricin (mixture of linear gramicidin and tyrocidine) applied to a column of basic aluminum oxide. The hydrolysate of labeled linear gramicidin on thin layer chromatography showed that L-(U-14C)serine was one of a precursor of ethanolamine moiety by autoradiography. L-(3-14C)serine generated formic acid in the presence of tetrahydrofolic acid by an enzyme fraction prepared with ammonium sulfate, and further formed ethanolamine binding to the protein. Formylvaline was biosynthesized by it with tetrahydrofolic acid and ATP, and subsequently released from the protein. 相似文献
6.
Four experiments were conducted to study 1) factors affecting porcine oocyte maturation in culture medium and 2) a new method for oocyte maturation outside the porcine body. In Experiment 1, five groups of oocytes were cultured in m-TCM199 or m-KRB medium for 24 to 28, 32 to 36 or 40 to 42 hours and then were fertilized in vitro. The cleavage rate (two to four-cell stage) of oocytes cultured for 32 to 36 hours was significantly higher than those of the other oocytes. The results indicate that a suitable culture period for the in vitro maturation of porcine oocytes is 32 to 36 hours. In Experiment 2, four groups of oocytes were cultured in m-KRB or m-KRB supplemented with PFF, PMSG or FSH for in vitro maturation, and the cleavage rates of oocytes were 7.94, 22.56, 30.23 and 23.26%, respectively, after in vitro fertilization. The results show that porcine follicular fluid (PFF) and gonadotrophins added to the culture medium promote porcine oocyte maturation in vitro. In Experiment 3, oocytes were cultured in m-KRB or m-TCM199, supplemented with both gonadotrophin and pocine folliclar fluid for maturation in vitro. After fertilization in vitro, the cleavage rates of oocytes were 26.32 and 27.93% for the two media. The results indicate that the difference between m-KRB and m-TCM199 was insignificant when the media were used to culture porcine oocytes. But there was a significant difference when PFF and gonadotrophins were added to the basic media. In Experiment 4, porcine oocytes were transferred into the reproductive tracts of other animals for maturation. After 34 to 36 hours, the oocytes were collected and fertilized in vitro. The cleavage rates of oocytes were 10.42, 28.45, 3.33 and 36.36%, respectively, for the oocytes matured in mouse uterine horns, rat uterine horns, rat oviducts or rabbit oviducts. The results show that porcine oocytes can be matured in the reproductive tracts of other animals. 相似文献
7.
Zhen Jiang Zhengkai Lu Shan Kou Teng Feng Yuanxin Wei Zibei Gao Defang Deng Jufeng Meng Chao-Po Lin Bin Zhou Hui Zhang 《Cell research》2021,31(4):485-487
Dear Editor,
Coronary artery disease is a leading cause of mortality and morbidity worldwide.Blockade of effective blood flow to heart muscles results in cardi... 相似文献
8.
赤霉菌超氧化物歧化酶的纯化及部分理化性质 总被引:5,自引:0,他引:5
采用加热、Sephadex G—100凝胶过滤及DEAE-Sephadex A-50柱层析的方法,提纯了赤霉菌的超氧化物歧化酶(SOD),纯酶比活力为2640U/mg蛋白,最大紫外吸收峰为276nm,为Mn-SOD,由二个亚基组成,亚基分子量为14.5kD。此外还报道了该酶的氨基酸组成。 相似文献
9.
Sheng-Bin Kou Gang Xu Xiao-Dan Jiang Ru-Xiang Xu Yan-Ping Tang Gang Xu Ying-Qian Cai Mou-Xuan Du Zhi-Cheng Xiao 《Cellular and molecular neurobiology》2010,30(2):275-282
Myelin-derived proteins, such as tenascin-R (TN-R), myelin associate glycoprotein (MAG), oligodendrocyte-myelin glycoprotein
(OMgp), and Nogo-A, inhibit the central nervous system regeneration. In this study, the DNA vaccine encoding for oligodendrocyte
and myelin-related antigens was employed to attenuate the axonal growth inhibitory properties of myelin in the setting of
spinal cord injury. Using a rat spinal cord dorsal hemisection model, the vaccine directed against the inhibitory epitopes
of Nogo-A, MAG, OMgp, and TN-R was administered intramuscularly once a week following spinal cord injury, supplemented with
local application of specific anti-sera against the four antigens. Anterograde labeling of dorsal column fibers showed active
axonal regeneration through the lesion site at the eighth week following the treatment in experimental group but not in control
groups. Light microscopic and ultrastructural analysis revealed that vaccination with these myelin-related antigens did not
lead to demyelinating disease. OMgp and TN-R levels were down-regulated at the lesion site together with a parallel increase
in growth-associated protein 43 levels in the treatment groups. This study reveals the effective approach of a DNA vaccine
strategy by attaining the special antibody to direct neutralization of the myelin inhibitors during spinal cord injury. 相似文献
10.
Two strains of Pseudomonus sp. having the extracellular catechol 1, 2-dioxygenase activity were selected from 112 bacterial strains. The conditions for enzyme production of the strains were examined. The optimal temperature and pH for enzyme formation were 30 degrees C and pH 6.8-7.0 respectively. Enzyme formation was enhanced by sodium benzoate, and was markedly inhibited by glucose, maltose and glycerol. Ammoniacal nitrogen sources were essential for cell growth and enzyme production. Sodium succinate was an effective inducer for enzyme formation. When the organism was grown in 0.15% sodium benzoate medium (pH 6.8-7.0) at 30 degrees C for 72 hours, about 10 units of catechol 1,2 dioxygenase per ml was obtained. 相似文献