Age-related changes in nitroxidergic neurons in some nuclei of rat medulla oblongata

The paper presents a comparative study of NO neurons in the solitary tract nucleus, giant-cell, and lateral reticular nuclei in rats at 4, 7, 10, 14, 30, 45, and 60 days old and 3, 6, 12, 18, 24 months old. We determine the active quantitative and qualitative changes that occur in NO-positive neurons in the studied nuclei during the course of postnatal development. A low level of enzyme activity is observed on the first day; it reaches a peak level around the first-third month, then slowly declines. The size and number of nitroxidergic neurons increases, while the relative cell density decreases until the third month of life. We reveal local differences in the ontogenetic development of NO neurons in the studied nuclei. Solitary tract neurons have the highest rate of development, while NO neurons of old animals undergo early and extreme changes as compared to other studied nuclei of rat medulla oblongata.     

Comparative characteristics of serotoninergic neurons in some nuclei of rat medulla

Using the immunohistochemical method, serotoninergic neurons were identified in eight medulla nuclei in the bulbar vasomotor centers of Wistar rats. Using morphometry, it was established that the proportion of serotoninergic neurons located in the projection of the studied nuclei varied at 17–26%; the value of this parameter only reached 34–40% in nuclei of the posterior raphe group. Single immunopositive cells able to perform integrative functions in the regulation of hemodynamics were identified between the nuclei, as well as between nuclei and conducting pathways.     

Receptor glomeruli and their ultrastructural organization in the arteries and pia mater of the brain and spinal cord of humans

Receptor glomeruli have been studied in the arterial walls of the pia mater of the human brain and spinal cord by means of the light and electron microscopy methods. Zone of their high concentration have been stated; three types of receptor glomeruli have been determined, as well as 3 types of ultrastructural organization of sensitive terminals.     

Protein-polymer grafts. II

Arginine hydrochloride reacts with equimolar 4-hydroxybenzil smoothly, in 70% ethanolic potassium hydroxide (0.5 m), at room temperature to produceN ⁵-[5-(p-hydroxphenyl)-4-oxo-5-phenyl-2-imidazolin-2-yl]-L-ornithine, in 70% yield. Purification was affected by paper chromatography. The pure product crystallizes with 1.5 moles of water. It resists boiling in concentrated hydrochloric acid and potassium hydroxide.     

Cystathionine beta-synthase in the structural elements of the human brain and spinal cord

Investigated by immunohistochemistry the presence and distribution of CBS in the nuclei of the spinal cord and brainstem in 8 mens of 18-44 years old, died from mechanical injury is not related to the central nervous system damage. Established that the CBS-neurons are revealed in all parts of stem and spinal cord, but their content in the studied cores, ranging from 0.9 to 17 %. Observed a definite relationship between the activity of CBS and the quantity of neurons. Large cells of motor nuclei often have a high and very high intensity of the reaction. In the sensory nuclei of a high proportion of small neurons with low activity of the enzyme.     

Histophysiology of tissue basophils of the cerebral dura mater after laser irradiation

By means of the histochemical and morphometric methods the reaction of the tissue basophils of the brain dura mater has been studied to the one-time and varying-duration (0.5 sec.-3 hr.) irradiation by a helium-neon laser of the wave length 632.8 nm, power density 0.76 mvt/sm2. It has been found, that the laser irradiation had a biostimulating effect upon the tissue basophils; the first peak of activity is in the case of a 3-second continuous irradiation; the second--from 15 min. to 1 hr. In symmetrical parts of the right (irradiated) and left (nonirradiated) regions of the dura very similar changes of the functional activity of the tissue basophils activity were seen.     

The functional morphology of the uterine capillary bed after synestrol administration

The capillary vessels of rats uterus was examined which were receiving for 7 days 0.2% solution of synoestrol i/m (2.0 mg per 1 kg of body weight). The animals were examined 1, 3, 5, 10, 20, 30, 60 days after the last injection of the solution. As the marker for the capillary vessels the histochemical method for finding the Mg2(+)-adenosintriphosphatase was used. The investigations have found prominent qualitative and quantitative changes (activity of enzyme, length and diameter of capillaries) not less than for two months after the last injection of the solution. Particularly prominent changes were found in endometrium between 10-20 days of the restorative period.